Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0620 and Shew_0621 are separated by 80 nucleotides Shew_0621 and Shew_0622 are separated by 60 nucleotides Shew_0622 and Shew_0623 are separated by 207 nucleotides Shew_0623 and Shew_0624 are separated by 70 nucleotides
Shew_0620: Shew_0620 - ion transport 2 domain-containing protein (RefSeq), at 705,768 to 706,562
_0620
Shew_0621: Shew_0621 - hypothetical protein (RefSeq), at 706,643 to 706,975
_0621
Shew_0622: Shew_0622 - hypothetical protein (RefSeq), at 707,036 to 707,680
_0622
Shew_0623: Shew_0623 - phage shock protein A, PspA (RefSeq), at 707,888 to 708,574
_0623
Shew_0624: Shew_0624 - hypothetical protein (RefSeq), at 708,645 to 709,058
_0624
Position (kb) 707
708 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 706.222 kb on - strand, within Shew_0620 at 706.768 kb on - strand, within Shew_0621 at 706.790 kb on - strand, within Shew_0621 at 706.831 kb on - strand, within Shew_0621 at 706.990 kb on - strand at 707.029 kb on + strand at 707.029 kb on + strand at 707.111 kb on - strand, within Shew_0622 at 707.495 kb on - strand, within Shew_0622 at 707.848 kb on + strand at 707.917 kb on - strand at 708.203 kb on - strand, within Shew_0623
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 706,222 - Shew_0620 0.57 +0.6 706,768 - Shew_0621 0.38 +0.3 706,790 - Shew_0621 0.44 -0.3 706,831 - Shew_0621 0.56 +0.1 706,990 - -0.2 707,029 + -0.1 707,029 + +0.3 707,111 - Shew_0622 0.12 -2.5 707,495 - Shew_0622 0.71 -0.3 707,848 + -2.3 707,917 - -1.9 708,203 - Shew_0623 0.46 -5.4
Or see this region's nucleotide sequence