Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0604 and Shew_0605 are separated by 263 nucleotides Shew_0605 and Shew_0606 are separated by 84 nucleotides Shew_0606 and Shew_0607 overlap by 4 nucleotides Shew_0607 and Shew_0608 are separated by 12 nucleotides
Shew_0604: Shew_0604 - DEAD/DEAH box helicase domain-containing protein (RefSeq), at 682,776 to 684,251
_0604
Shew_0605: Shew_0605 - hypothetical protein (RefSeq), at 684,515 to 684,850
_0605
Shew_0606: Shew_0606 - polysulphide reductase, NrfD (RefSeq), at 684,935 to 685,876
_0606
Shew_0607: Shew_0607 - 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein (RefSeq), at 685,873 to 686,445
_0607
Shew_0608: Shew_0608 - molybdopterin oxidoreductase (RefSeq), at 686,458 to 688,740
_0608
Position (kb) 684
685
686 Strain fitness (log2 ratio) -2
-1
0
1
2 at 684.162 kb on - strand at 684.955 kb on - strand at 685.228 kb on + strand, within Shew_0606 at 685.228 kb on + strand, within Shew_0606 at 685.236 kb on - strand, within Shew_0606 at 685.280 kb on + strand, within Shew_0606 at 685.379 kb on - strand, within Shew_0606 at 685.657 kb on + strand, within Shew_0606 at 685.675 kb on - strand, within Shew_0606 at 685.697 kb on + strand, within Shew_0606 at 685.782 kb on - strand at 685.914 kb on + strand at 685.922 kb on - strand at 685.922 kb on - strand at 685.937 kb on + strand, within Shew_0607 at 686.846 kb on - strand, within Shew_0608
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 684,162 - +0.3 684,955 - -0.7 685,228 + Shew_0606 0.31 -0.1 685,228 + Shew_0606 0.31 -0.8 685,236 - Shew_0606 0.32 +1.1 685,280 + Shew_0606 0.37 +0.4 685,379 - Shew_0606 0.47 +0.6 685,657 + Shew_0606 0.77 +0.1 685,675 - Shew_0606 0.79 +1.1 685,697 + Shew_0606 0.81 +1.9 685,782 - +0.3 685,914 + +0.3 685,922 - -1.3 685,922 - +0.0 685,937 + Shew_0607 0.11 -2.6 686,846 - Shew_0608 0.17 -0.7
Or see this region's nucleotide sequence