Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0539 and Shew_0540 are separated by 15 nucleotides Shew_0540 and Shew_0541 are separated by 49 nucleotides Shew_0541 and Shew_0542 are separated by 12 nucleotides Shew_0542 and Shew_0543 are separated by 4 nucleotides
Shew_0539: Shew_0539 - hypothetical protein (RefSeq), at 611,049 to 611,408
_0539
Shew_0540: Shew_0540 - enoyl-CoA hydratase/isomerase (RefSeq), at 611,424 to 612,161
_0540
Shew_0541: Shew_0541 - hypothetical protein (RefSeq), at 612,211 to 612,678
_0541
Shew_0542: Shew_0542 - hypothetical protein (RefSeq), at 612,691 to 613,662
_0542
Shew_0543: Shew_0543 - hypothetical protein (RefSeq), at 613,667 to 614,425
_0543
Position (kb) 612
613 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 611.385 kb on + strand at 611.385 kb on + strand at 611.385 kb on + strand at 611.817 kb on - strand, within Shew_0540 at 611.892 kb on - strand, within Shew_0540 at 612.080 kb on + strand, within Shew_0540 at 612.560 kb on + strand, within Shew_0541 at 612.689 kb on - strand at 612.704 kb on - strand at 612.785 kb on + strand at 612.785 kb on + strand at 612.819 kb on + strand, within Shew_0542 at 613.228 kb on + strand, within Shew_0542 at 613.295 kb on - strand, within Shew_0542 at 613.425 kb on - strand, within Shew_0542 at 613.490 kb on - strand, within Shew_0542 at 613.564 kb on - strand, within Shew_0542 at 613.581 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 611,385 + -2.3 611,385 + -0.7 611,385 + +1.6 611,817 - Shew_0540 0.53 +0.3 611,892 - Shew_0540 0.63 -1.3 612,080 + Shew_0540 0.89 -3.5 612,560 + Shew_0541 0.75 -0.8 612,689 - -3.1 612,704 - +1.2 612,785 + -0.9 612,785 + -0.0 612,819 + Shew_0542 0.13 -0.8 613,228 + Shew_0542 0.55 -0.3 613,295 - Shew_0542 0.62 -2.6 613,425 - Shew_0542 0.76 -0.1 613,490 - Shew_0542 0.82 -0.6 613,564 - Shew_0542 0.90 -1.9 613,581 - +0.7
Or see this region's nucleotide sequence