Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0533 and Shew_0534 are separated by 202 nucleotides Shew_0534 and Shew_0535 are separated by 72 nucleotides Shew_0535 and Shew_0536 are separated by 195 nucleotides
Shew_0533: Shew_0533 - excinuclease ABC, A subunit (RefSeq), at 603,761 to 606,586
_0533
Shew_0534: Shew_0534 - major facilitator transporter (RefSeq), at 606,789 to 608,156
_0534
Shew_0535: Shew_0535 - single-strand binding protein (RefSeq), at 608,229 to 608,891
_0535
Shew_0536: Shew_0536 - tRNA-dihydrouridine synthase A (RefSeq), at 609,087 to 610,076
_0536
Position (kb) 606
607
608
609 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 605.962 kb on + strand, within Shew_0533 at 606.076 kb on - strand, within Shew_0533 at 606.094 kb on - strand, within Shew_0533 at 606.370 kb on + strand at 606.442 kb on - strand at 606.466 kb on - strand at 606.493 kb on + strand at 606.571 kb on + strand at 606.593 kb on + strand at 606.806 kb on + strand at 606.806 kb on + strand at 606.806 kb on + strand at 606.806 kb on + strand at 606.806 kb on + strand at 606.806 kb on + strand at 606.814 kb on - strand at 606.814 kb on - strand at 607.367 kb on - strand, within Shew_0534 at 607.597 kb on - strand, within Shew_0534 at 607.615 kb on + strand, within Shew_0534 at 607.783 kb on - strand, within Shew_0534 at 607.952 kb on + strand, within Shew_0534 at 608.962 kb on - strand at 608.972 kb on - strand at 608.990 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 605,962 + Shew_0533 0.78 -5.4 606,076 - Shew_0533 0.82 -3.1 606,094 - Shew_0533 0.83 -6.7 606,370 + -3.1 606,442 - -6.5 606,466 - -5.2 606,493 + -5.1 606,571 + -3.4 606,593 + -3.1 606,806 + -1.9 606,806 + -0.9 606,806 + -2.5 606,806 + -2.1 606,806 + -1.9 606,806 + -2.7 606,814 - -2.2 606,814 - -0.3 607,367 - Shew_0534 0.42 -1.9 607,597 - Shew_0534 0.59 -2.1 607,615 + Shew_0534 0.60 -2.0 607,783 - Shew_0534 0.73 -4.2 607,952 + Shew_0534 0.85 -0.6 608,962 - +0.3 608,972 - +0.1 608,990 + +0.1
Or see this region's nucleotide sequence