Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0484 and Shew_0485 are separated by 36 nucleotides Shew_0485 and Shew_0486 are separated by 92 nucleotides Shew_0486 and Shew_0487 are separated by 27 nucleotides Shew_0487 and Shew_0488 are separated by 10 nucleotides Shew_0488 and Shew_0489 are separated by 277 nucleotides
Shew_0484: Shew_0484 - hypothetical protein (RefSeq), at 541,500 to 542,771
_0484
Shew_0485: Shew_0485 - cold-shock DNA-binding domain-containing protein (RefSeq), at 542,808 to 543,425
_0485
Shew_0486: Shew_0486 - permease (RefSeq), at 543,518 to 544,534
_0486
Shew_0487: Shew_0487 - redox-active disulfide protein 2 (RefSeq), at 544,562 to 544,810
_0487
Shew_0488: Shew_0488 - ArsR family transcriptional regulator (RefSeq), at 544,821 to 545,225
_0488
Shew_0489: Shew_0489 - hypothetical protein (RefSeq), at 545,503 to 545,931
_0489
Position (kb) 543
544
545 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 542.637 kb on + strand, within Shew_0484 at 542.639 kb on - strand, within Shew_0484 at 542.669 kb on - strand at 542.851 kb on - strand at 543.095 kb on + strand, within Shew_0485 at 543.559 kb on + strand at 543.567 kb on - strand at 543.679 kb on - strand, within Shew_0486 at 543.679 kb on - strand, within Shew_0486 at 543.778 kb on + strand, within Shew_0486 at 544.116 kb on + strand, within Shew_0486 at 544.116 kb on + strand, within Shew_0486 at 544.250 kb on + strand, within Shew_0486 at 544.321 kb on + strand, within Shew_0486 at 544.321 kb on + strand, within Shew_0486 at 544.402 kb on + strand, within Shew_0486 at 544.412 kb on + strand, within Shew_0486 at 544.542 kb on + strand at 544.542 kb on + strand at 544.542 kb on + strand at 544.542 kb on + strand at 544.542 kb on + strand at 544.550 kb on - strand at 544.550 kb on - strand at 544.550 kb on - strand at 544.631 kb on + strand, within Shew_0487 at 544.835 kb on + strand at 544.942 kb on - strand, within Shew_0488 at 545.058 kb on - strand, within Shew_0488 at 545.394 kb on + strand at 545.465 kb on + strand at 545.520 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 542,637 + Shew_0484 0.89 -0.6 542,639 - Shew_0484 0.90 -0.3 542,669 - -0.0 542,851 - +0.0 543,095 + Shew_0485 0.46 -1.6 543,559 + +1.0 543,567 - +0.2 543,679 - Shew_0486 0.16 +0.2 543,679 - Shew_0486 0.16 -1.7 543,778 + Shew_0486 0.26 +0.4 544,116 + Shew_0486 0.59 +0.7 544,116 + Shew_0486 0.59 +0.3 544,250 + Shew_0486 0.72 -0.1 544,321 + Shew_0486 0.79 -0.4 544,321 + Shew_0486 0.79 +0.8 544,402 + Shew_0486 0.87 +1.0 544,412 + Shew_0486 0.88 +3.3 544,542 + -0.1 544,542 + +0.3 544,542 + -0.3 544,542 + +0.7 544,542 + -0.2 544,550 - -0.3 544,550 - +0.6 544,550 - +0.4 544,631 + Shew_0487 0.28 -0.3 544,835 + -0.0 544,942 - Shew_0488 0.30 -0.2 545,058 - Shew_0488 0.59 -0.8 545,394 + -2.3 545,465 + +0.9 545,520 + -0.2
Or see this region's nucleotide sequence