Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0453 and Shew_0454 are separated by 405 nucleotides Shew_0454 and Shew_0455 are separated by 165 nucleotides Shew_0455 and Shew_0456 are separated by 47 nucleotides
Shew_0453: Shew_0453 - response regulator receiver protein (RefSeq), at 503,415 to 504,410
_0453
Shew_0454: Shew_0454 - enoyl-CoA hydratase (RefSeq), at 504,816 to 505,625
_0454
Shew_0455: Shew_0455 - hypothetical protein (RefSeq), at 505,791 to 506,294
_0455
Shew_0456: Shew_0456 - hypothetical protein (RefSeq), at 506,342 to 507,226
_0456
Position (kb) 504
505
506 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 504.038 kb on + strand, within Shew_0453 at 504.055 kb on + strand, within Shew_0453 at 504.839 kb on + strand at 504.842 kb on - strand at 504.862 kb on + strand at 504.911 kb on + strand, within Shew_0454 at 505.019 kb on - strand, within Shew_0454 at 505.324 kb on + strand, within Shew_0454 at 506.038 kb on + strand, within Shew_0455 at 506.209 kb on - strand, within Shew_0455 at 506.383 kb on - strand at 506.406 kb on + strand at 506.542 kb on - strand, within Shew_0456 at 506.561 kb on - strand, within Shew_0456 at 506.617 kb on - strand, within Shew_0456 at 506.621 kb on + strand, within Shew_0456
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 504,038 + Shew_0453 0.63 -3.7 504,055 + Shew_0453 0.64 +0.4 504,839 + +0.4 504,842 - -0.5 504,862 + +1.9 504,911 + Shew_0454 0.12 -1.9 505,019 - Shew_0454 0.25 -0.1 505,324 + Shew_0454 0.63 +0.0 506,038 + Shew_0455 0.49 +0.1 506,209 - Shew_0455 0.83 +0.2 506,383 - -0.3 506,406 + +0.2 506,542 - Shew_0456 0.23 -0.3 506,561 - Shew_0456 0.25 +0.1 506,617 - Shew_0456 0.31 +2.8 506,621 + Shew_0456 0.32 +0.9
Or see this region's nucleotide sequence