Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0443 and Shew_0444 are separated by 144 nucleotides Shew_0444 and Shew_0445 are separated by 124 nucleotides
Shew_0443: Shew_0443 - formate--tetrahydrofolate ligase (RefSeq), at 493,192 to 494,886
_0443
Shew_0444: Shew_0444 - MscS mechanosensitive ion channel (RefSeq), at 495,031 to 496,653
_0444
Shew_0445: Shew_0445 - hypothetical protein (RefSeq), at 496,778 to 497,113
_0445
Position (kb)
495
496
497 Strain fitness (log2 ratio)
-2
-1
0
1 at 494.107 kb on - strand, within Shew_0443 at 494.729 kb on + strand at 494.800 kb on - strand at 494.880 kb on + strand at 494.880 kb on + strand at 494.880 kb on + strand at 494.880 kb on + strand at 494.880 kb on + strand at 494.888 kb on - strand at 495.159 kb on + strand at 495.328 kb on + strand, within Shew_0444 at 495.448 kb on - strand, within Shew_0444 at 495.549 kb on + strand, within Shew_0444 at 495.585 kb on - strand, within Shew_0444 at 496.039 kb on + strand, within Shew_0444 at 496.097 kb on - strand, within Shew_0444 at 496.139 kb on - strand, within Shew_0444 at 496.407 kb on - strand, within Shew_0444 at 496.433 kb on - strand, within Shew_0444 at 496.606 kb on + strand at 496.702 kb on - strand at 496.737 kb on + strand at 496.796 kb on + strand at 496.984 kb on + strand, within Shew_0445 at 497.051 kb on - strand, within Shew_0445 at 497.221 kb on + strand at 497.229 kb on - strand at 497.229 kb on - strand at 497.250 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 494,107 - Shew_0443 0.54 -0.2 494,729 + -0.1 494,800 - +0.1 494,880 + +1.6 494,880 + +0.3 494,880 + +0.1 494,880 + -0.3 494,880 + +0.3 494,888 - +0.1 495,159 + +0.7 495,328 + Shew_0444 0.18 -0.8 495,448 - Shew_0444 0.26 +0.2 495,549 + Shew_0444 0.32 -1.2 495,585 - Shew_0444 0.34 -0.3 496,039 + Shew_0444 0.62 -0.4 496,097 - Shew_0444 0.66 -1.7 496,139 - Shew_0444 0.68 +1.5 496,407 - Shew_0444 0.85 -0.1 496,433 - Shew_0444 0.86 -2.3 496,606 + +0.2 496,702 - +0.4 496,737 + -0.3 496,796 + -1.1 496,984 + Shew_0445 0.61 +0.2 497,051 - Shew_0445 0.81 -0.7 497,221 + +0.3 497,229 - +0.8 497,229 - +0.3 497,250 - -0.3
Or see this region's nucleotide sequence