Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0395 and Shew_0396 are separated by 89 nucleotides Shew_0396 and Shew_0397 are separated by 14 nucleotides Shew_0397 and Shew_0398 are separated by 146 nucleotides Shew_0398 and Shew_0399 overlap by 11 nucleotides
Shew_0395: Shew_0395 - methylation site containing protein (RefSeq), at 446,032 to 446,667
_0395
Shew_0396: Shew_0396 - methylation site containing protein (RefSeq), at 446,757 to 447,266
_0396
Shew_0397: Shew_0397 - methylation site containing protein (RefSeq), at 447,281 to 447,772
_0397
Shew_0398: Shew_0398 - MSHA pilin protein MshC (RefSeq), at 447,919 to 448,368
_0398
Shew_0399: Shew_0399 - methylation site containing protein (RefSeq), at 448,358 to 448,909
_0399
Position (kb) 447
448 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 446.335 kb on + strand, within Shew_0395 at 446.641 kb on + strand at 446.781 kb on - strand at 447.023 kb on - strand, within Shew_0396 at 447.033 kb on - strand, within Shew_0396 at 447.144 kb on - strand, within Shew_0396 at 447.229 kb on + strand at 447.548 kb on + strand, within Shew_0397 at 447.785 kb on + strand at 447.793 kb on - strand at 448.031 kb on + strand, within Shew_0398 at 448.039 kb on - strand, within Shew_0398 at 448.398 kb on + strand at 448.533 kb on + strand, within Shew_0399 at 448.648 kb on + strand, within Shew_0399 at 448.661 kb on - strand, within Shew_0399 at 448.661 kb on - strand, within Shew_0399 at 448.727 kb on - strand, within Shew_0399 at 448.743 kb on - strand, within Shew_0399
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 446,335 + Shew_0395 0.48 +0.0 446,641 + +0.5 446,781 - -1.5 447,023 - Shew_0396 0.52 +2.0 447,033 - Shew_0396 0.54 -1.8 447,144 - Shew_0396 0.76 +0.2 447,229 + +1.1 447,548 + Shew_0397 0.54 -0.2 447,785 + +0.7 447,793 - +0.7 448,031 + Shew_0398 0.25 -2.4 448,039 - Shew_0398 0.27 -0.7 448,398 + -0.5 448,533 + Shew_0399 0.32 +0.4 448,648 + Shew_0399 0.53 +0.1 448,661 - Shew_0399 0.55 -0.4 448,661 - Shew_0399 0.55 -0.5 448,727 - Shew_0399 0.67 -1.7 448,743 - Shew_0399 0.70 -3.1
Or see this region's nucleotide sequence