Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0357 and Shew_0358 are separated by 180 nucleotides Shew_0358 and fre are separated by 69 nucleotides fre and Shew_0360 are separated by 134 nucleotides
Shew_0357: Shew_0357 - copper ABC transporter, permease protein (RefSeq), at 405,496 to 406,347
_0357
Shew_0358: Shew_0358 - transcriptional regulator, CadC (RefSeq), at 406,528 to 407,808
_0358
Shew_0359: fre - FMN reductase (RefSeq), at 407,878 to 408,576
fre
Shew_0360: Shew_0360 - UbiD family decarboxylase (RefSeq), at 408,711 to 410,192
_0360
Position (kb) 406
407
408 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 405.681 kb on - strand, within Shew_0357 at 405.683 kb on + strand, within Shew_0357 at 406.026 kb on - strand, within Shew_0357 at 406.078 kb on - strand, within Shew_0357 at 406.104 kb on - strand, within Shew_0357 at 406.305 kb on + strand at 406.393 kb on - strand at 407.263 kb on - strand, within Shew_0358 at 407.503 kb on + strand, within Shew_0358 at 407.503 kb on + strand, within Shew_0358 at 407.594 kb on + strand, within Shew_0358 at 407.771 kb on - strand at 407.810 kb on - strand at 407.853 kb on + strand at 408.011 kb on - strand, within fre at 408.109 kb on - strand, within fre at 408.161 kb on - strand, within fre at 408.237 kb on + strand, within fre
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 405,681 - Shew_0357 0.22 +0.4 405,683 + Shew_0357 0.22 +0.3 406,026 - Shew_0357 0.62 +1.5 406,078 - Shew_0357 0.68 +0.2 406,104 - Shew_0357 0.71 -0.2 406,305 + +0.7 406,393 - -0.3 407,263 - Shew_0358 0.57 +0.4 407,503 + Shew_0358 0.76 +1.4 407,503 + Shew_0358 0.76 -1.8 407,594 + Shew_0358 0.83 +0.5 407,771 - +0.6 407,810 - -0.3 407,853 + -0.4 408,011 - fre Shew_0359 0.19 -3.3 408,109 - fre Shew_0359 0.33 -2.5 408,161 - fre Shew_0359 0.40 -2.2 408,237 + fre Shew_0359 0.51 -2.0
Or see this region's nucleotide sequence