Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0353 and Shew_0354 are separated by 54 nucleotides Shew_0354 and Shew_0355 are separated by 10 nucleotides Shew_0355 and Shew_0356 overlap by 4 nucleotides Shew_0356 and Shew_0357 overlap by 4 nucleotides
Shew_0353: Shew_0353 - polysulphide reductase, NrfD (RefSeq), at 401,714 to 402,661
_0353
Shew_0354: Shew_0354 - NosL family protein (RefSeq), at 402,716 to 403,204
_0354
Shew_0355: Shew_0355 - periplasmic copper-binding (RefSeq), at 403,215 to 404,573
_0355
Shew_0356: Shew_0356 - ABC transporter-related protein (RefSeq), at 404,570 to 405,499
_0356
Shew_0357: Shew_0357 - copper ABC transporter, permease protein (RefSeq), at 405,496 to 406,347
_0357
Position (kb)
403
404
405 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 402.632 kb on - strand at 402.641 kb on + strand at 402.702 kb on + strand at 402.780 kb on + strand, within Shew_0354 at 403.124 kb on + strand, within Shew_0354 at 403.130 kb on - strand, within Shew_0354 at 403.149 kb on - strand, within Shew_0354 at 403.173 kb on + strand at 403.187 kb on - strand at 403.288 kb on + strand at 403.432 kb on - strand, within Shew_0355 at 403.456 kb on + strand, within Shew_0355 at 403.921 kb on - strand, within Shew_0355 at 404.277 kb on + strand, within Shew_0355 at 404.416 kb on + strand, within Shew_0355 at 404.597 kb on - strand at 404.721 kb on + strand, within Shew_0356 at 404.744 kb on - strand, within Shew_0356 at 404.853 kb on + strand, within Shew_0356 at 404.853 kb on + strand, within Shew_0356 at 404.861 kb on + strand, within Shew_0356 at 404.861 kb on - strand, within Shew_0356 at 404.861 kb on + strand, within Shew_0356 at 404.965 kb on - strand, within Shew_0356 at 405.017 kb on - strand, within Shew_0356 at 405.019 kb on + strand, within Shew_0356 at 405.022 kb on - strand, within Shew_0356 at 405.141 kb on + strand, within Shew_0356 at 405.149 kb on - strand, within Shew_0356 at 405.175 kb on - strand, within Shew_0356 at 405.175 kb on - strand, within Shew_0356 at 405.190 kb on + strand, within Shew_0356 at 405.213 kb on + strand, within Shew_0356 at 405.418 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 402,632 - +0.5 402,641 + +0.3 402,702 + -0.1 402,780 + Shew_0354 0.13 -0.2 403,124 + Shew_0354 0.83 -0.6 403,130 - Shew_0354 0.85 -0.2 403,149 - Shew_0354 0.89 -0.2 403,173 + +0.8 403,187 - -4.7 403,288 + +0.5 403,432 - Shew_0355 0.16 +0.2 403,456 + Shew_0355 0.18 -0.9 403,921 - Shew_0355 0.52 +0.5 404,277 + Shew_0355 0.78 -1.2 404,416 + Shew_0355 0.88 -0.4 404,597 - +0.0 404,721 + Shew_0356 0.16 +0.4 404,744 - Shew_0356 0.19 -3.4 404,853 + Shew_0356 0.30 +0.6 404,853 + Shew_0356 0.30 -0.3 404,861 + Shew_0356 0.31 +0.3 404,861 - Shew_0356 0.31 +0.6 404,861 + Shew_0356 0.31 +1.8 404,965 - Shew_0356 0.42 +0.7 405,017 - Shew_0356 0.48 +0.4 405,019 + Shew_0356 0.48 -1.4 405,022 - Shew_0356 0.49 +0.8 405,141 + Shew_0356 0.61 +0.4 405,149 - Shew_0356 0.62 +0.4 405,175 - Shew_0356 0.65 +0.7 405,175 - Shew_0356 0.65 +0.4 405,190 + Shew_0356 0.67 +0.5 405,213 + Shew_0356 0.69 +2.8 405,418 + -1.2
Or see this region's nucleotide sequence