Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0332 and Shew_0333 are separated by 144 nucleotides Shew_0333 and Shew_0334 are separated by 46 nucleotides
Shew_0332: Shew_0332 - fumarate reductase cytochrome b-556 subunit (RefSeq), at 377,079 to 377,801
_0332
Shew_0333: Shew_0333 - fumarate reductase respiratory complex, transmembrane subunit (RefSeq), at 377,946 to 378,617
_0333
Shew_0334: Shew_0334 - fumarate reductase flavoprotein subunit (RefSeq), at 378,664 to 380,670
_0334
Position (kb)
377
378
379 Strain fitness (log2 ratio)
-2
-1
0
1 at 377.129 kb on - strand at 377.129 kb on - strand at 377.256 kb on + strand, within Shew_0332 at 377.292 kb on + strand, within Shew_0332 at 377.292 kb on + strand, within Shew_0332 at 377.300 kb on - strand, within Shew_0332 at 377.300 kb on - strand, within Shew_0332 at 377.646 kb on + strand, within Shew_0332 at 377.812 kb on - strand at 378.023 kb on - strand, within Shew_0333 at 378.037 kb on - strand, within Shew_0333 at 378.047 kb on - strand, within Shew_0333 at 378.047 kb on - strand, within Shew_0333 at 378.416 kb on + strand, within Shew_0333 at 378.576 kb on + strand at 378.576 kb on + strand at 378.771 kb on + strand at 378.828 kb on + strand at 378.836 kb on - strand at 379.118 kb on - strand, within Shew_0334 at 379.143 kb on + strand, within Shew_0334 at 379.143 kb on + strand, within Shew_0334 at 379.143 kb on + strand, within Shew_0334 at 379.143 kb on + strand, within Shew_0334 at 379.143 kb on + strand, within Shew_0334 at 379.151 kb on - strand, within Shew_0334 at 379.421 kb on - strand, within Shew_0334
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 377,129 - -2.4 377,129 - +0.1 377,256 + Shew_0332 0.24 +0.1 377,292 + Shew_0332 0.29 +1.2 377,292 + Shew_0332 0.29 +0.3 377,300 - Shew_0332 0.31 +0.8 377,300 - Shew_0332 0.31 +0.6 377,646 + Shew_0332 0.78 +0.5 377,812 - -0.8 378,023 - Shew_0333 0.11 +0.3 378,037 - Shew_0333 0.14 -0.4 378,047 - Shew_0333 0.15 +0.0 378,047 - Shew_0333 0.15 -1.7 378,416 + Shew_0333 0.70 -0.6 378,576 + +0.0 378,576 + -1.2 378,771 + +0.0 378,828 + +0.5 378,836 - -0.6 379,118 - Shew_0334 0.23 +0.3 379,143 + Shew_0334 0.24 -0.6 379,143 + Shew_0334 0.24 -1.9 379,143 + Shew_0334 0.24 +1.4 379,143 + Shew_0334 0.24 +0.3 379,143 + Shew_0334 0.24 +0.4 379,151 - Shew_0334 0.24 -1.2 379,421 - Shew_0334 0.38 +0.3
Or see this region's nucleotide sequence