Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0326 and Shew_0327 are separated by 101 nucleotides Shew_0327 and Shew_0328 are separated by 112 nucleotides Shew_0328 and Shew_0329 are separated by 262 nucleotides Shew_0329 and Shew_0330 are separated by 17 nucleotides
Shew_0326: Shew_0326 - HAD family hydrolase (RefSeq), at 371,917 to 372,639
_0326
Shew_0327: Shew_0327 - HupE/UreJ protein (RefSeq), at 372,741 to 373,316
_0327
Shew_0328: Shew_0328 - hypothetical protein (RefSeq), at 373,429 to 374,145
_0328
Shew_0329: Shew_0329 - Fis family transcriptional regulator (RefSeq), at 374,408 to 374,713
_0329
Shew_0330: Shew_0330 - nifR3 family TIM-barrel protein (RefSeq), at 374,731 to 375,699
_0330
Position (kb) 373
374
375 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 372.474 kb on - strand, within Shew_0326 at 372.750 kb on + strand at 373.140 kb on + strand, within Shew_0327 at 373.145 kb on + strand, within Shew_0327 at 373.148 kb on - strand, within Shew_0327 at 373.554 kb on + strand, within Shew_0328 at 373.647 kb on - strand, within Shew_0328 at 374.191 kb on + strand at 374.191 kb on + strand at 374.199 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 372,474 - Shew_0326 0.77 -3.7 372,750 + -1.4 373,140 + Shew_0327 0.69 -1.8 373,145 + Shew_0327 0.70 +1.4 373,148 - Shew_0327 0.71 +1.7 373,554 + Shew_0328 0.17 +0.0 373,647 - Shew_0328 0.30 -0.6 374,191 + -0.6 374,191 + -1.4 374,199 - +0.3
Or see this region's nucleotide sequence