Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0262 and Shew_0263 are separated by 70 nucleotides Shew_0263 and Shew_0264 overlap by 4 nucleotides Shew_0264 and Shew_0265 are separated by 129 nucleotides
Shew_0262: Shew_0262 - hypothetical protein (RefSeq), at 280,966 to 282,420
_0262
Shew_0263: Shew_0263 - hypothetical protein (RefSeq), at 282,491 to 282,796
_0263
Shew_0264: Shew_0264 - serine/threonine protein kinase (RefSeq), at 282,793 to 283,794
_0264
Shew_0265: Shew_0265 - DSBA oxidoreductase (RefSeq), at 283,924 to 284,535
_0265
Position (kb) 282
283
284 Strain fitness (log2 ratio) -1
0
1
2 at 281.923 kb on + strand, within Shew_0262 at 282.023 kb on + strand, within Shew_0262 at 282.296 kb on - strand at 282.601 kb on + strand, within Shew_0263 at 283.020 kb on + strand, within Shew_0264 at 283.087 kb on + strand, within Shew_0264 at 283.157 kb on + strand, within Shew_0264 at 283.157 kb on + strand, within Shew_0264 at 283.165 kb on - strand, within Shew_0264 at 283.347 kb on - strand, within Shew_0264 at 283.356 kb on + strand, within Shew_0264 at 284.091 kb on - strand, within Shew_0265 at 284.211 kb on - strand, within Shew_0265 at 284.366 kb on + strand, within Shew_0265 at 284.486 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 281,923 + Shew_0262 0.66 +0.4 282,023 + Shew_0262 0.73 +0.8 282,296 - +0.5 282,601 + Shew_0263 0.36 -0.4 283,020 + Shew_0264 0.23 -0.5 283,087 + Shew_0264 0.29 +0.6 283,157 + Shew_0264 0.36 +1.4 283,157 + Shew_0264 0.36 -0.6 283,165 - Shew_0264 0.37 +0.0 283,347 - Shew_0264 0.55 +1.3 283,356 + Shew_0264 0.56 +2.2 284,091 - Shew_0265 0.27 +0.2 284,211 - Shew_0265 0.47 -1.0 284,366 + Shew_0265 0.72 +0.1 284,486 - -0.2
Or see this region's nucleotide sequence