Experiment: Cisplatin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0231 and Shew_0232 are separated by 192 nucleotides Shew_0232 and Shew_0233 are separated by 130 nucleotides Shew_0233 and Shew_0234 are separated by 74 nucleotides Shew_0234 and Shew_0235 are separated by 13 nucleotides
Shew_0231: Shew_0231 - hypothetical protein (RefSeq), at 245,669 to 246,940
_0231
Shew_0232: Shew_0232 - hypothetical protein (RefSeq), at 247,133 to 247,528
_0232
Shew_0233: Shew_0233 - hypothetical protein (RefSeq), at 247,659 to 250,001
_0233
Shew_0234: Shew_0234 - hypothetical protein (RefSeq), at 250,076 to 250,435
_0234
Shew_0235: Shew_0235 - isochorismatase hydrolase (RefSeq), at 250,449 to 251,399
_0235
Position (kb) 247
248
249
250
251 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 246.690 kb on - strand, within Shew_0231 at 247.136 kb on - strand at 247.235 kb on - strand, within Shew_0232 at 247.264 kb on - strand, within Shew_0232 at 247.415 kb on - strand, within Shew_0232 at 247.415 kb on - strand, within Shew_0232 at 247.420 kb on - strand, within Shew_0232 at 247.587 kb on - strand at 247.685 kb on - strand at 247.745 kb on + strand at 248.066 kb on + strand, within Shew_0233 at 248.076 kb on - strand, within Shew_0233 at 248.083 kb on + strand, within Shew_0233 at 248.100 kb on - strand, within Shew_0233 at 248.296 kb on - strand, within Shew_0233 at 248.504 kb on + strand, within Shew_0233 at 248.504 kb on + strand, within Shew_0233 at 248.542 kb on + strand, within Shew_0233 at 248.806 kb on - strand, within Shew_0233 at 248.862 kb on + strand, within Shew_0233 at 248.862 kb on + strand, within Shew_0233 at 248.870 kb on - strand, within Shew_0233 at 249.280 kb on + strand, within Shew_0233 at 249.635 kb on - strand, within Shew_0233 at 249.635 kb on - strand, within Shew_0233 at 249.818 kb on + strand at 249.982 kb on - strand at 250.144 kb on + strand, within Shew_0234 at 250.151 kb on + strand, within Shew_0234 at 250.179 kb on - strand, within Shew_0234 at 250.224 kb on + strand, within Shew_0234 at 250.481 kb on + strand at 250.659 kb on + strand, within Shew_0235 at 250.659 kb on + strand, within Shew_0235 at 250.662 kb on - strand, within Shew_0235 at 250.667 kb on - strand, within Shew_0235 at 250.667 kb on - strand, within Shew_0235 at 250.936 kb on + strand, within Shew_0235
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 1 mg/ml remove 246,690 - Shew_0231 0.80 -1.3 247,136 - -0.1 247,235 - Shew_0232 0.26 +0.3 247,264 - Shew_0232 0.33 +1.7 247,415 - Shew_0232 0.71 -2.5 247,415 - Shew_0232 0.71 +0.4 247,420 - Shew_0232 0.72 +0.4 247,587 - -1.1 247,685 - +0.0 247,745 + -0.0 248,066 + Shew_0233 0.17 -0.9 248,076 - Shew_0233 0.18 +0.2 248,083 + Shew_0233 0.18 +0.4 248,100 - Shew_0233 0.19 -2.9 248,296 - Shew_0233 0.27 +0.6 248,504 + Shew_0233 0.36 -0.2 248,504 + Shew_0233 0.36 +1.2 248,542 + Shew_0233 0.38 +0.4 248,806 - Shew_0233 0.49 -2.5 248,862 + Shew_0233 0.51 +0.5 248,862 + Shew_0233 0.51 -3.2 248,870 - Shew_0233 0.52 -0.4 249,280 + Shew_0233 0.69 -1.6 249,635 - Shew_0233 0.84 -0.5 249,635 - Shew_0233 0.84 +0.1 249,818 + +0.0 249,982 - -1.2 250,144 + Shew_0234 0.19 +0.3 250,151 + Shew_0234 0.21 +0.1 250,179 - Shew_0234 0.29 -2.2 250,224 + Shew_0234 0.41 +1.0 250,481 + -0.4 250,659 + Shew_0235 0.22 -1.0 250,659 + Shew_0235 0.22 +0.2 250,662 - Shew_0235 0.22 -0.3 250,667 - Shew_0235 0.23 +0.0 250,667 - Shew_0235 0.23 +0.8 250,936 + Shew_0235 0.51 -0.1
Or see this region's nucleotide sequence