Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_519

Experiment: D-Mannose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_518 and Pf6N2E2_519 are separated by 39 nucleotidesPf6N2E2_519 and Pf6N2E2_520 are separated by 10 nucleotidesPf6N2E2_520 and Pf6N2E2_521 are separated by 15 nucleotidesPf6N2E2_521 and Pf6N2E2_522 are separated by 51 nucleotides Pf6N2E2_518 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 588,491 to 589,501 _518 Pf6N2E2_519 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 589,541 to 590,593 _519 Pf6N2E2_520 - hypothetical protein, at 590,604 to 590,720 _520 Pf6N2E2_521 - hypothetical protein, at 590,736 to 590,888 _521 Pf6N2E2_522 - Inositol transport system sugar-binding protein, at 590,940 to 591,866 _522 Position (kb) 589 590 591Strain fitness (log2 ratio) -2 -1 0 1 2at 588.541 kb on + strandat 588.653 kb on + strand, within Pf6N2E2_518at 588.876 kb on - strand, within Pf6N2E2_518at 588.876 kb on - strand, within Pf6N2E2_518at 588.876 kb on - strand, within Pf6N2E2_518at 588.981 kb on - strand, within Pf6N2E2_518at 588.981 kb on - strand, within Pf6N2E2_518at 588.981 kb on - strand, within Pf6N2E2_518at 589.055 kb on + strand, within Pf6N2E2_518at 589.055 kb on + strand, within Pf6N2E2_518at 589.055 kb on + strand, within Pf6N2E2_518at 589.055 kb on + strand, within Pf6N2E2_518at 589.055 kb on + strand, within Pf6N2E2_518at 589.055 kb on + strand, within Pf6N2E2_518at 589.055 kb on + strand, within Pf6N2E2_518at 589.055 kb on + strand, within Pf6N2E2_518at 589.056 kb on - strand, within Pf6N2E2_518at 589.056 kb on - strand, within Pf6N2E2_518at 589.056 kb on - strand, within Pf6N2E2_518at 589.188 kb on - strand, within Pf6N2E2_518at 589.329 kb on - strand, within Pf6N2E2_518at 589.329 kb on - strand, within Pf6N2E2_518at 589.529 kb on + strandat 589.529 kb on + strandat 589.529 kb on + strandat 589.529 kb on + strandat 589.530 kb on - strandat 589.530 kb on - strandat 589.530 kb on - strandat 589.618 kb on + strandat 589.618 kb on + strandat 589.619 kb on - strandat 589.619 kb on - strandat 589.998 kb on - strand, within Pf6N2E2_519at 590.121 kb on - strand, within Pf6N2E2_519at 590.261 kb on + strand, within Pf6N2E2_519at 590.262 kb on - strand, within Pf6N2E2_519at 590.262 kb on - strand, within Pf6N2E2_519at 590.262 kb on - strand, within Pf6N2E2_519at 590.429 kb on + strand, within Pf6N2E2_519at 590.430 kb on - strand, within Pf6N2E2_519at 590.430 kb on - strand, within Pf6N2E2_519at 590.430 kb on - strand, within Pf6N2E2_519at 590.430 kb on - strand, within Pf6N2E2_519at 590.430 kb on - strand, within Pf6N2E2_519at 590.430 kb on - strand, within Pf6N2E2_519at 590.724 kb on + strandat 590.724 kb on + strandat 590.724 kb on + strandat 590.724 kb on + strandat 590.725 kb on - strandat 590.729 kb on - strandat 590.763 kb on + strand, within Pf6N2E2_521at 590.763 kb on + strand, within Pf6N2E2_521at 590.763 kb on + strand, within Pf6N2E2_521at 590.763 kb on + strand, within Pf6N2E2_521at 590.764 kb on - strand, within Pf6N2E2_521at 590.764 kb on - strand, within Pf6N2E2_521at 590.907 kb on + strandat 591.060 kb on + strand, within Pf6N2E2_522at 591.061 kb on - strand, within Pf6N2E2_522at 591.061 kb on - strand, within Pf6N2E2_522at 591.478 kb on - strand, within Pf6N2E2_522

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Mannose (C)
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588,541 + +0.2
588,653 + Pf6N2E2_518 0.16 +0.8
588,876 - Pf6N2E2_518 0.38 -2.4
588,876 - Pf6N2E2_518 0.38 -0.5
588,876 - Pf6N2E2_518 0.38 -2.2
588,981 - Pf6N2E2_518 0.48 +0.1
588,981 - Pf6N2E2_518 0.48 -0.2
588,981 - Pf6N2E2_518 0.48 -1.1
589,055 + Pf6N2E2_518 0.56 +0.2
589,055 + Pf6N2E2_518 0.56 -0.2
589,055 + Pf6N2E2_518 0.56 +1.8
589,055 + Pf6N2E2_518 0.56 +0.9
589,055 + Pf6N2E2_518 0.56 -0.4
589,055 + Pf6N2E2_518 0.56 -1.0
589,055 + Pf6N2E2_518 0.56 -0.1
589,055 + Pf6N2E2_518 0.56 +0.5
589,056 - Pf6N2E2_518 0.56 -0.8
589,056 - Pf6N2E2_518 0.56 -0.7
589,056 - Pf6N2E2_518 0.56 -0.3
589,188 - Pf6N2E2_518 0.69 -0.5
589,329 - Pf6N2E2_518 0.83 -1.5
589,329 - Pf6N2E2_518 0.83 -1.2
589,529 + +0.8
589,529 + +0.1
589,529 + +0.6
589,529 + +0.5
589,530 - -0.1
589,530 - -0.3
589,530 - +0.8
589,618 + -0.6
589,618 + -1.1
589,619 - -0.4
589,619 - -1.9
589,998 - Pf6N2E2_519 0.43 -0.5
590,121 - Pf6N2E2_519 0.55 -0.9
590,261 + Pf6N2E2_519 0.68 +0.1
590,262 - Pf6N2E2_519 0.68 -1.3
590,262 - Pf6N2E2_519 0.68 -0.9
590,262 - Pf6N2E2_519 0.68 +0.6
590,429 + Pf6N2E2_519 0.84 -0.8
590,430 - Pf6N2E2_519 0.84 -0.8
590,430 - Pf6N2E2_519 0.84 -2.2
590,430 - Pf6N2E2_519 0.84 -1.7
590,430 - Pf6N2E2_519 0.84 +0.3
590,430 - Pf6N2E2_519 0.84 -0.4
590,430 - Pf6N2E2_519 0.84 -2.5
590,724 + -1.0
590,724 + +2.1
590,724 + -0.5
590,724 + +1.7
590,725 - -1.3
590,729 - +0.9
590,763 + Pf6N2E2_521 0.18 +0.7
590,763 + Pf6N2E2_521 0.18 -0.3
590,763 + Pf6N2E2_521 0.18 +0.6
590,763 + Pf6N2E2_521 0.18 -0.3
590,764 - Pf6N2E2_521 0.18 -0.4
590,764 - Pf6N2E2_521 0.18 +0.9
590,907 + -0.0
591,060 + Pf6N2E2_522 0.13 -0.8
591,061 - Pf6N2E2_522 0.13 -1.1
591,061 - Pf6N2E2_522 0.13 -2.3
591,478 - Pf6N2E2_522 0.58 +0.9

Or see this region's nucleotide sequence