Experiment: D-Mannose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2371 and Pf6N2E2_2372 overlap by 4 nucleotides Pf6N2E2_2372 and Pf6N2E2_2373 are separated by 4 nucleotides Pf6N2E2_2373 and Pf6N2E2_2374 are separated by 243 nucleotides
Pf6N2E2_2371 - Type III secretion outermembrane contact sensing protein (YopN,Yop4b,LcrE), at 2,805,670 to 2,806,773
_2371
Pf6N2E2_2372 - Type III secretion outermembrane pore forming protein (YscC,MxiD,HrcC, InvG), at 2,806,770 to 2,808,425
_2372
Pf6N2E2_2373 - Type III secretion thermoregulatory protein (LcrF,VirF,transcription regulation of virulence plasmid), at 2,808,430 to 2,809,167
_2373
Pf6N2E2_2374 - Nitrate ABC transporter, nitrate-binding protein, at 2,809,411 to 2,810,622
_2374
Position (kb)
2806
2807
2808
2809 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6 at 2805.908 kb on + strand, within Pf6N2E2_2371 at 2806.119 kb on + strand, within Pf6N2E2_2371 at 2806.120 kb on - strand, within Pf6N2E2_2371 at 2806.792 kb on + strand at 2806.792 kb on + strand at 2806.830 kb on + strand at 2806.830 kb on + strand at 2806.830 kb on + strand at 2806.831 kb on - strand at 2806.831 kb on - strand at 2807.061 kb on - strand, within Pf6N2E2_2372 at 2807.190 kb on + strand, within Pf6N2E2_2372 at 2807.190 kb on + strand, within Pf6N2E2_2372 at 2807.191 kb on - strand, within Pf6N2E2_2372 at 2807.191 kb on - strand, within Pf6N2E2_2372 at 2807.209 kb on + strand, within Pf6N2E2_2372 at 2807.209 kb on + strand, within Pf6N2E2_2372 at 2807.210 kb on - strand, within Pf6N2E2_2372 at 2807.257 kb on + strand, within Pf6N2E2_2372 at 2807.258 kb on - strand, within Pf6N2E2_2372 at 2807.258 kb on - strand, within Pf6N2E2_2372 at 2807.258 kb on - strand, within Pf6N2E2_2372 at 2807.258 kb on - strand, within Pf6N2E2_2372 at 2807.258 kb on - strand, within Pf6N2E2_2372 at 2807.258 kb on - strand, within Pf6N2E2_2372 at 2807.258 kb on - strand, within Pf6N2E2_2372 at 2807.258 kb on - strand, within Pf6N2E2_2372 at 2807.852 kb on - strand, within Pf6N2E2_2372 at 2807.971 kb on + strand, within Pf6N2E2_2372 at 2807.971 kb on + strand, within Pf6N2E2_2372 at 2807.972 kb on - strand, within Pf6N2E2_2372 at 2807.972 kb on - strand, within Pf6N2E2_2372 at 2807.972 kb on - strand, within Pf6N2E2_2372 at 2808.128 kb on - strand, within Pf6N2E2_2372 at 2808.128 kb on - strand, within Pf6N2E2_2372 at 2808.128 kb on - strand, within Pf6N2E2_2372 at 2808.133 kb on + strand, within Pf6N2E2_2372 at 2808.133 kb on + strand, within Pf6N2E2_2372 at 2808.133 kb on + strand, within Pf6N2E2_2372 at 2808.133 kb on + strand, within Pf6N2E2_2372 at 2808.133 kb on + strand, within Pf6N2E2_2372 at 2808.134 kb on - strand, within Pf6N2E2_2372 at 2808.134 kb on - strand, within Pf6N2E2_2372 at 2808.134 kb on - strand, within Pf6N2E2_2372 at 2808.154 kb on + strand, within Pf6N2E2_2372 at 2808.334 kb on + strand at 2808.334 kb on + strand at 2808.334 kb on + strand at 2808.334 kb on + strand at 2808.335 kb on - strand at 2808.377 kb on + strand at 2808.447 kb on - strand at 2808.447 kb on - strand at 2808.447 kb on - strand at 2808.447 kb on - strand at 2808.727 kb on + strand, within Pf6N2E2_2373 at 2808.736 kb on + strand, within Pf6N2E2_2373 at 2808.786 kb on - strand, within Pf6N2E2_2373 at 2809.183 kb on + strand at 2809.183 kb on + strand at 2809.184 kb on - strand at 2809.184 kb on - strand at 2809.187 kb on + strand at 2809.187 kb on + strand at 2809.187 kb on + strand at 2809.187 kb on + strand at 2809.187 kb on + strand at 2809.188 kb on - strand at 2809.265 kb on + strand at 2809.265 kb on + strand at 2809.265 kb on + strand at 2809.266 kb on - strand at 2809.266 kb on - strand at 2809.266 kb on - strand at 2809.331 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Mannose (C) remove 2,805,908 + Pf6N2E2_2371 0.22 -0.9 2,806,119 + Pf6N2E2_2371 0.41 +1.0 2,806,120 - Pf6N2E2_2371 0.41 -0.2 2,806,792 + +0.7 2,806,792 + -0.1 2,806,830 + +0.0 2,806,830 + -1.0 2,806,830 + +0.7 2,806,831 - -1.4 2,806,831 - -0.0 2,807,061 - Pf6N2E2_2372 0.18 +0.2 2,807,190 + Pf6N2E2_2372 0.25 +1.0 2,807,190 + Pf6N2E2_2372 0.25 +0.4 2,807,191 - Pf6N2E2_2372 0.25 -0.2 2,807,191 - Pf6N2E2_2372 0.25 -0.5 2,807,209 + Pf6N2E2_2372 0.27 +0.0 2,807,209 + Pf6N2E2_2372 0.27 +0.2 2,807,210 - Pf6N2E2_2372 0.27 +0.2 2,807,257 + Pf6N2E2_2372 0.29 -0.4 2,807,258 - Pf6N2E2_2372 0.29 +1.7 2,807,258 - Pf6N2E2_2372 0.29 +0.7 2,807,258 - Pf6N2E2_2372 0.29 -0.7 2,807,258 - Pf6N2E2_2372 0.29 +0.4 2,807,258 - Pf6N2E2_2372 0.29 +0.0 2,807,258 - Pf6N2E2_2372 0.29 -1.1 2,807,258 - Pf6N2E2_2372 0.29 +6.2 2,807,258 - Pf6N2E2_2372 0.29 +1.5 2,807,852 - Pf6N2E2_2372 0.65 +0.0 2,807,971 + Pf6N2E2_2372 0.73 +0.9 2,807,971 + Pf6N2E2_2372 0.73 +0.7 2,807,972 - Pf6N2E2_2372 0.73 +0.4 2,807,972 - Pf6N2E2_2372 0.73 -0.2 2,807,972 - Pf6N2E2_2372 0.73 +0.6 2,808,128 - Pf6N2E2_2372 0.82 -0.4 2,808,128 - Pf6N2E2_2372 0.82 -0.7 2,808,128 - Pf6N2E2_2372 0.82 +0.3 2,808,133 + Pf6N2E2_2372 0.82 +0.8 2,808,133 + Pf6N2E2_2372 0.82 -0.4 2,808,133 + Pf6N2E2_2372 0.82 +1.3 2,808,133 + Pf6N2E2_2372 0.82 +0.0 2,808,133 + Pf6N2E2_2372 0.82 +0.7 2,808,134 - Pf6N2E2_2372 0.82 -0.2 2,808,134 - Pf6N2E2_2372 0.82 -0.2 2,808,134 - Pf6N2E2_2372 0.82 +0.6 2,808,154 + Pf6N2E2_2372 0.84 -1.0 2,808,334 + -1.3 2,808,334 + +0.4 2,808,334 + -0.1 2,808,334 + +0.5 2,808,335 - +0.3 2,808,377 + +1.2 2,808,447 - +0.1 2,808,447 - +0.0 2,808,447 - +0.9 2,808,447 - +0.2 2,808,727 + Pf6N2E2_2373 0.40 +0.0 2,808,736 + Pf6N2E2_2373 0.41 +0.6 2,808,786 - Pf6N2E2_2373 0.48 -0.1 2,809,183 + -0.3 2,809,183 + -0.3 2,809,184 - +0.6 2,809,184 - -2.0 2,809,187 + +0.4 2,809,187 + -0.7 2,809,187 + +0.4 2,809,187 + -0.0 2,809,187 + +1.0 2,809,188 - +0.1 2,809,265 + +0.2 2,809,265 + +0.0 2,809,265 + -0.2 2,809,266 - -0.2 2,809,266 - -0.7 2,809,266 - +0.6 2,809,331 - +0.5
Or see this region's nucleotide sequence