Experiment: D-Mannose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_209 and Pf6N2E2_210 are separated by 181 nucleotides Pf6N2E2_210 and Pf6N2E2_211 are separated by 92 nucleotides Pf6N2E2_211 and Pf6N2E2_212 overlap by 8 nucleotides
Pf6N2E2_209 - O-antigen export system permease protein RfbD, at 263,376 to 264,194
_209
Pf6N2E2_210 - Teichoic acid export ATP-binding protein TagH (EC 3.6.3.40), at 264,376 to 267,099
_210
Pf6N2E2_211 - SanC, at 267,192 to 267,779
_211
Pf6N2E2_212 - Glycosyl transferase, group 2 family protein, at 267,772 to 269,946
_212
Position (kb)
264
265
266
267
268 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 263.379 kb on + strand at 263.380 kb on - strand at 263.380 kb on - strand at 263.380 kb on - strand at 263.380 kb on - strand at 263.588 kb on - strand, within Pf6N2E2_209 at 263.624 kb on + strand, within Pf6N2E2_209 at 263.625 kb on - strand, within Pf6N2E2_209 at 263.656 kb on + strand, within Pf6N2E2_209 at 263.657 kb on - strand, within Pf6N2E2_209 at 263.664 kb on + strand, within Pf6N2E2_209 at 263.770 kb on + strand, within Pf6N2E2_209 at 263.770 kb on + strand, within Pf6N2E2_209 at 263.770 kb on + strand, within Pf6N2E2_209 at 263.771 kb on - strand at 263.872 kb on + strand, within Pf6N2E2_209 at 263.872 kb on + strand, within Pf6N2E2_209 at 263.873 kb on - strand, within Pf6N2E2_209 at 264.032 kb on - strand, within Pf6N2E2_209 at 264.069 kb on + strand, within Pf6N2E2_209 at 264.069 kb on + strand, within Pf6N2E2_209 at 264.069 kb on + strand, within Pf6N2E2_209 at 264.069 kb on + strand, within Pf6N2E2_209 at 264.069 kb on + strand, within Pf6N2E2_209 at 264.070 kb on - strand, within Pf6N2E2_209 at 264.070 kb on - strand, within Pf6N2E2_209 at 264.070 kb on - strand, within Pf6N2E2_209 at 264.070 kb on - strand, within Pf6N2E2_209 at 264.070 kb on - strand, within Pf6N2E2_209 at 264.070 kb on - strand, within Pf6N2E2_209 at 264.094 kb on + strand, within Pf6N2E2_209 at 264.127 kb on + strand at 264.127 kb on + strand at 264.127 kb on + strand at 264.128 kb on - strand at 264.128 kb on - strand at 264.250 kb on + strand at 264.261 kb on + strand at 264.261 kb on + strand at 264.262 kb on - strand at 264.296 kb on + strand at 264.296 kb on + strand at 264.297 kb on - strand at 264.344 kb on + strand at 264.430 kb on + strand at 264.430 kb on + strand at 264.430 kb on + strand at 264.431 kb on - strand at 264.431 kb on - strand at 264.709 kb on + strand, within Pf6N2E2_210 at 264.709 kb on + strand, within Pf6N2E2_210 at 264.709 kb on + strand, within Pf6N2E2_210 at 264.820 kb on + strand, within Pf6N2E2_210 at 265.033 kb on + strand, within Pf6N2E2_210 at 265.033 kb on + strand, within Pf6N2E2_210 at 265.034 kb on - strand, within Pf6N2E2_210 at 265.255 kb on + strand, within Pf6N2E2_210 at 265.255 kb on + strand, within Pf6N2E2_210 at 265.261 kb on + strand, within Pf6N2E2_210 at 265.702 kb on - strand, within Pf6N2E2_210 at 265.703 kb on + strand, within Pf6N2E2_210 at 265.722 kb on - strand, within Pf6N2E2_210 at 265.810 kb on + strand, within Pf6N2E2_210 at 265.874 kb on + strand, within Pf6N2E2_210 at 265.929 kb on + strand, within Pf6N2E2_210 at 266.036 kb on + strand, within Pf6N2E2_210 at 266.037 kb on - strand, within Pf6N2E2_210 at 266.067 kb on - strand, within Pf6N2E2_210 at 266.290 kb on + strand, within Pf6N2E2_210 at 266.291 kb on - strand, within Pf6N2E2_210 at 266.291 kb on - strand, within Pf6N2E2_210 at 266.291 kb on - strand, within Pf6N2E2_210 at 266.291 kb on - strand, within Pf6N2E2_210 at 266.291 kb on - strand, within Pf6N2E2_210 at 266.291 kb on - strand, within Pf6N2E2_210 at 266.291 kb on - strand, within Pf6N2E2_210 at 266.330 kb on + strand, within Pf6N2E2_210 at 266.368 kb on + strand, within Pf6N2E2_210 at 266.520 kb on + strand, within Pf6N2E2_210 at 266.520 kb on + strand, within Pf6N2E2_210 at 266.521 kb on - strand, within Pf6N2E2_210 at 266.521 kb on - strand, within Pf6N2E2_210 at 266.521 kb on - strand, within Pf6N2E2_210 at 266.521 kb on - strand, within Pf6N2E2_210 at 266.524 kb on + strand, within Pf6N2E2_210 at 266.524 kb on + strand, within Pf6N2E2_210 at 266.525 kb on - strand, within Pf6N2E2_210 at 266.525 kb on - strand, within Pf6N2E2_210 at 266.596 kb on + strand, within Pf6N2E2_210 at 266.597 kb on - strand, within Pf6N2E2_210 at 266.597 kb on - strand, within Pf6N2E2_210 at 266.597 kb on - strand, within Pf6N2E2_210 at 266.597 kb on - strand, within Pf6N2E2_210 at 267.097 kb on + strand at 267.097 kb on + strand at 267.097 kb on + strand at 267.097 kb on + strand at 267.098 kb on - strand at 267.098 kb on - strand at 267.098 kb on - strand at 267.098 kb on - strand at 267.256 kb on - strand, within Pf6N2E2_211 at 267.256 kb on - strand, within Pf6N2E2_211 at 267.256 kb on - strand, within Pf6N2E2_211 at 267.261 kb on + strand, within Pf6N2E2_211 at 267.261 kb on + strand, within Pf6N2E2_211 at 267.261 kb on + strand, within Pf6N2E2_211 at 267.261 kb on + strand, within Pf6N2E2_211 at 267.261 kb on + strand, within Pf6N2E2_211 at 267.262 kb on - strand, within Pf6N2E2_211 at 267.262 kb on - strand, within Pf6N2E2_211 at 267.262 kb on - strand, within Pf6N2E2_211 at 267.504 kb on + strand, within Pf6N2E2_211 at 267.505 kb on - strand, within Pf6N2E2_211 at 267.623 kb on - strand, within Pf6N2E2_211 at 267.706 kb on - strand, within Pf6N2E2_211 at 267.777 kb on + strand at 267.777 kb on + strand at 267.778 kb on - strand at 267.850 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Mannose (C) remove 263,379 + -0.6 263,380 - +0.3 263,380 - -0.4 263,380 - -2.1 263,380 - -2.2 263,588 - Pf6N2E2_209 0.26 -1.5 263,624 + Pf6N2E2_209 0.30 -1.7 263,625 - Pf6N2E2_209 0.30 -0.2 263,656 + Pf6N2E2_209 0.34 -0.8 263,657 - Pf6N2E2_209 0.34 +0.7 263,664 + Pf6N2E2_209 0.35 +0.8 263,770 + Pf6N2E2_209 0.48 -0.8 263,770 + Pf6N2E2_209 0.48 +0.2 263,770 + Pf6N2E2_209 0.48 -1.0 263,771 - +1.1 263,872 + Pf6N2E2_209 0.61 -1.1 263,872 + Pf6N2E2_209 0.61 +0.1 263,873 - Pf6N2E2_209 0.61 +0.9 264,032 - Pf6N2E2_209 0.80 -1.5 264,069 + Pf6N2E2_209 0.85 -2.5 264,069 + Pf6N2E2_209 0.85 -0.5 264,069 + Pf6N2E2_209 0.85 -0.2 264,069 + Pf6N2E2_209 0.85 +1.2 264,069 + Pf6N2E2_209 0.85 -0.9 264,070 - Pf6N2E2_209 0.85 -0.0 264,070 - Pf6N2E2_209 0.85 +1.7 264,070 - Pf6N2E2_209 0.85 +0.3 264,070 - Pf6N2E2_209 0.85 -1.6 264,070 - Pf6N2E2_209 0.85 +1.2 264,070 - Pf6N2E2_209 0.85 +0.1 264,094 + Pf6N2E2_209 0.88 +0.6 264,127 + -0.7 264,127 + +0.7 264,127 + +0.6 264,128 - -0.1 264,128 - +0.6 264,250 + +1.9 264,261 + -0.9 264,261 + +0.6 264,262 - +0.9 264,296 + -0.1 264,296 + -0.3 264,297 - +0.1 264,344 + +2.1 264,430 + -1.6 264,430 + -1.1 264,430 + +0.3 264,431 - +0.7 264,431 - -0.1 264,709 + Pf6N2E2_210 0.12 +0.4 264,709 + Pf6N2E2_210 0.12 +0.2 264,709 + Pf6N2E2_210 0.12 -0.8 264,820 + Pf6N2E2_210 0.16 -0.8 265,033 + Pf6N2E2_210 0.24 +0.5 265,033 + Pf6N2E2_210 0.24 +0.8 265,034 - Pf6N2E2_210 0.24 +1.2 265,255 + Pf6N2E2_210 0.32 -2.0 265,255 + Pf6N2E2_210 0.32 +1.3 265,261 + Pf6N2E2_210 0.32 +1.1 265,702 - Pf6N2E2_210 0.49 +0.7 265,703 + Pf6N2E2_210 0.49 +1.5 265,722 - Pf6N2E2_210 0.49 -1.2 265,810 + Pf6N2E2_210 0.53 +2.8 265,874 + Pf6N2E2_210 0.55 -0.5 265,929 + Pf6N2E2_210 0.57 -0.5 266,036 + Pf6N2E2_210 0.61 +0.1 266,037 - Pf6N2E2_210 0.61 +1.1 266,067 - Pf6N2E2_210 0.62 +1.5 266,290 + Pf6N2E2_210 0.70 +0.7 266,291 - Pf6N2E2_210 0.70 +1.4 266,291 - Pf6N2E2_210 0.70 +0.4 266,291 - Pf6N2E2_210 0.70 -0.1 266,291 - Pf6N2E2_210 0.70 -0.0 266,291 - Pf6N2E2_210 0.70 -0.3 266,291 - Pf6N2E2_210 0.70 +0.4 266,291 - Pf6N2E2_210 0.70 -0.7 266,330 + Pf6N2E2_210 0.72 +0.2 266,368 + Pf6N2E2_210 0.73 -0.9 266,520 + Pf6N2E2_210 0.79 +2.9 266,520 + Pf6N2E2_210 0.79 +0.1 266,521 - Pf6N2E2_210 0.79 +0.5 266,521 - Pf6N2E2_210 0.79 +0.8 266,521 - Pf6N2E2_210 0.79 -0.2 266,521 - Pf6N2E2_210 0.79 -0.1 266,524 + Pf6N2E2_210 0.79 +1.0 266,524 + Pf6N2E2_210 0.79 +1.2 266,525 - Pf6N2E2_210 0.79 +1.1 266,525 - Pf6N2E2_210 0.79 +0.5 266,596 + Pf6N2E2_210 0.81 +1.0 266,597 - Pf6N2E2_210 0.82 +0.7 266,597 - Pf6N2E2_210 0.82 +0.4 266,597 - Pf6N2E2_210 0.82 -1.1 266,597 - Pf6N2E2_210 0.82 +2.1 267,097 + -0.2 267,097 + +0.3 267,097 + +0.6 267,097 + +0.5 267,098 - -1.5 267,098 - +2.3 267,098 - -0.2 267,098 - -0.5 267,256 - Pf6N2E2_211 0.11 +1.4 267,256 - Pf6N2E2_211 0.11 -0.1 267,256 - Pf6N2E2_211 0.11 -0.1 267,261 + Pf6N2E2_211 0.12 -0.2 267,261 + Pf6N2E2_211 0.12 -0.2 267,261 + Pf6N2E2_211 0.12 -0.3 267,261 + Pf6N2E2_211 0.12 -1.9 267,261 + Pf6N2E2_211 0.12 +0.6 267,262 - Pf6N2E2_211 0.12 +0.9 267,262 - Pf6N2E2_211 0.12 -0.3 267,262 - Pf6N2E2_211 0.12 +0.1 267,504 + Pf6N2E2_211 0.53 -0.3 267,505 - Pf6N2E2_211 0.53 +1.0 267,623 - Pf6N2E2_211 0.73 +0.4 267,706 - Pf6N2E2_211 0.87 +0.2 267,777 + -1.2 267,777 + +0.6 267,778 - -1.3 267,850 - +0.5
Or see this region's nucleotide sequence