Experiment: D-Mannose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1957 and Pf6N2E2_1958 are separated by 170 nucleotides Pf6N2E2_1958 and Pf6N2E2_1959 are separated by 188 nucleotides Pf6N2E2_1959 and Pf6N2E2_1960 are separated by 57 nucleotides
Pf6N2E2_1957 - putative inner membrane protein, at 2,321,749 to 2,323,065
_1957
Pf6N2E2_1958 - Predicted dinucleotide-binding enzymes, at 2,323,236 to 2,323,778
_1958
Pf6N2E2_1959 - D-sorbitol dehydrogenase (EC 1.1.1.14) (from data) , at 2,323,967 to 2,324,740
_1959
Pf6N2E2_1960 - ABC transporter for D-sorbitol, ATPase component (from data) , at 2,324,798 to 2,325,895
_1960
Position (kb)
2323
2324
2325 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2322.967 kb on - strand at 2322.967 kb on - strand at 2322.967 kb on - strand at 2323.029 kb on + strand at 2323.071 kb on - strand at 2323.201 kb on + strand at 2323.201 kb on + strand at 2323.202 kb on - strand at 2323.202 kb on - strand at 2323.459 kb on + strand, within Pf6N2E2_1958 at 2323.459 kb on + strand, within Pf6N2E2_1958 at 2323.459 kb on + strand, within Pf6N2E2_1958 at 2323.459 kb on + strand, within Pf6N2E2_1958 at 2323.462 kb on - strand, within Pf6N2E2_1958 at 2323.507 kb on - strand, within Pf6N2E2_1958 at 2323.777 kb on - strand at 2323.777 kb on - strand at 2323.800 kb on + strand at 2323.804 kb on + strand at 2323.805 kb on - strand at 2323.805 kb on - strand at 2323.805 kb on - strand at 2323.824 kb on + strand at 2323.999 kb on - strand at 2324.016 kb on + strand at 2324.123 kb on + strand, within Pf6N2E2_1959 at 2324.251 kb on + strand, within Pf6N2E2_1959 at 2324.578 kb on - strand, within Pf6N2E2_1959 at 2324.637 kb on - strand, within Pf6N2E2_1959 at 2324.664 kb on + strand at 2324.664 kb on + strand at 2324.664 kb on + strand at 2324.665 kb on - strand at 2324.665 kb on - strand at 2324.665 kb on - strand at 2324.665 kb on - strand at 2324.722 kb on + strand at 2324.748 kb on - strand at 2324.881 kb on - strand at 2324.881 kb on - strand at 2325.232 kb on - strand, within Pf6N2E2_1960 at 2325.232 kb on - strand, within Pf6N2E2_1960 at 2325.330 kb on + strand, within Pf6N2E2_1960 at 2325.331 kb on - strand, within Pf6N2E2_1960 at 2325.331 kb on - strand, within Pf6N2E2_1960 at 2325.355 kb on + strand, within Pf6N2E2_1960 at 2325.387 kb on - strand, within Pf6N2E2_1960 at 2325.502 kb on + strand, within Pf6N2E2_1960 at 2325.502 kb on + strand, within Pf6N2E2_1960 at 2325.503 kb on - strand, within Pf6N2E2_1960 at 2325.645 kb on + strand, within Pf6N2E2_1960 at 2325.645 kb on + strand, within Pf6N2E2_1960 at 2325.645 kb on + strand, within Pf6N2E2_1960 at 2325.646 kb on - strand, within Pf6N2E2_1960 at 2325.646 kb on - strand, within Pf6N2E2_1960 at 2325.646 kb on - strand, within Pf6N2E2_1960
Per-strain Table
Position Strand Gene LocusTag Fraction D-Mannose (C) remove 2,322,967 - +0.5 2,322,967 - -0.3 2,322,967 - -0.4 2,323,029 + +1.0 2,323,071 - +0.3 2,323,201 + +0.4 2,323,201 + -0.6 2,323,202 - -0.3 2,323,202 - +0.8 2,323,459 + Pf6N2E2_1958 0.41 -0.1 2,323,459 + Pf6N2E2_1958 0.41 -2.8 2,323,459 + Pf6N2E2_1958 0.41 +0.9 2,323,459 + Pf6N2E2_1958 0.41 -1.4 2,323,462 - Pf6N2E2_1958 0.42 +0.2 2,323,507 - Pf6N2E2_1958 0.50 -0.2 2,323,777 - -0.4 2,323,777 - -0.5 2,323,800 + -0.6 2,323,804 + -0.4 2,323,805 - -1.1 2,323,805 - -0.2 2,323,805 - +1.3 2,323,824 + -0.1 2,323,999 - -1.1 2,324,016 + +2.3 2,324,123 + Pf6N2E2_1959 0.20 +0.3 2,324,251 + Pf6N2E2_1959 0.37 -1.5 2,324,578 - Pf6N2E2_1959 0.79 +0.7 2,324,637 - Pf6N2E2_1959 0.87 +0.4 2,324,664 + -0.3 2,324,664 + -1.0 2,324,664 + +1.0 2,324,665 - -2.7 2,324,665 - -0.5 2,324,665 - -0.1 2,324,665 - +0.6 2,324,722 + +0.1 2,324,748 - -0.8 2,324,881 - +0.1 2,324,881 - +1.2 2,325,232 - Pf6N2E2_1960 0.40 -1.9 2,325,232 - Pf6N2E2_1960 0.40 +0.4 2,325,330 + Pf6N2E2_1960 0.48 +1.5 2,325,331 - Pf6N2E2_1960 0.49 -0.6 2,325,331 - Pf6N2E2_1960 0.49 +0.7 2,325,355 + Pf6N2E2_1960 0.51 -0.7 2,325,387 - Pf6N2E2_1960 0.54 -0.5 2,325,502 + Pf6N2E2_1960 0.64 +1.1 2,325,502 + Pf6N2E2_1960 0.64 -1.3 2,325,503 - Pf6N2E2_1960 0.64 -1.3 2,325,645 + Pf6N2E2_1960 0.77 +1.0 2,325,645 + Pf6N2E2_1960 0.77 +1.1 2,325,645 + Pf6N2E2_1960 0.77 -0.5 2,325,646 - Pf6N2E2_1960 0.77 -0.2 2,325,646 - Pf6N2E2_1960 0.77 +0.4 2,325,646 - Pf6N2E2_1960 0.77 -0.6
Or see this region's nucleotide sequence