Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_104

Experiment: D-Mannose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_103 and Pf6N2E2_104 overlap by 1 nucleotidesPf6N2E2_104 and Pf6N2E2_105 overlap by 4 nucleotidesPf6N2E2_105 and Pf6N2E2_106 are separated by 10 nucleotides Pf6N2E2_103 - Various polyols ABC transporter, ATP-binding component, at 148,544 to 149,638 _103 Pf6N2E2_104 - Glycerol-3-phosphate ABC transporter, ATP-binding protein UgpC (TC 3.A.1.1.3), at 149,638 to 150,747 _104 Pf6N2E2_105 - Glycerol-3-phosphate ABC transporter, permease protein UgpA (TC 3.A.1.1.3), at 150,744 to 151,610 _105 Pf6N2E2_106 - Glycerol-3-phosphate ABC transporter, permease protein UgpE (TC 3.A.1.1.3), at 151,621 to 152,421 _106 Position (kb) 149 150 151Strain fitness (log2 ratio) -2 -1 0 1 2at 148.652 kb on - strandat 149.037 kb on - strand, within Pf6N2E2_103at 149.037 kb on - strand, within Pf6N2E2_103at 149.204 kb on + strand, within Pf6N2E2_103at 149.383 kb on + strand, within Pf6N2E2_103at 149.383 kb on + strand, within Pf6N2E2_103at 149.384 kb on - strand, within Pf6N2E2_103at 149.384 kb on - strand, within Pf6N2E2_103at 149.525 kb on + strand, within Pf6N2E2_103at 149.525 kb on + strand, within Pf6N2E2_103at 149.552 kb on + strandat 149.552 kb on + strandat 149.553 kb on - strandat 149.675 kb on - strandat 149.675 kb on - strandat 149.914 kb on + strand, within Pf6N2E2_104at 149.914 kb on + strand, within Pf6N2E2_104at 149.914 kb on + strand, within Pf6N2E2_104at 149.915 kb on - strand, within Pf6N2E2_104at 149.915 kb on - strand, within Pf6N2E2_104at 149.915 kb on - strand, within Pf6N2E2_104at 149.942 kb on + strand, within Pf6N2E2_104at 149.943 kb on - strand, within Pf6N2E2_104at 149.943 kb on - strand, within Pf6N2E2_104at 150.223 kb on + strand, within Pf6N2E2_104at 150.224 kb on - strand, within Pf6N2E2_104at 150.280 kb on + strand, within Pf6N2E2_104at 150.281 kb on - strand, within Pf6N2E2_104at 150.281 kb on - strand, within Pf6N2E2_104at 150.532 kb on + strand, within Pf6N2E2_104at 150.533 kb on - strand, within Pf6N2E2_104at 150.583 kb on + strand, within Pf6N2E2_104at 150.583 kb on + strand, within Pf6N2E2_104at 150.584 kb on - strand, within Pf6N2E2_104at 150.584 kb on - strand, within Pf6N2E2_104at 150.627 kb on - strand, within Pf6N2E2_104at 150.667 kb on + strandat 150.667 kb on + strandat 150.667 kb on + strandat 150.701 kb on - strandat 150.712 kb on + strandat 150.712 kb on + strandat 150.879 kb on + strand, within Pf6N2E2_105at 151.167 kb on + strand, within Pf6N2E2_105at 151.167 kb on + strand, within Pf6N2E2_105at 151.168 kb on - strand, within Pf6N2E2_105at 151.168 kb on - strand, within Pf6N2E2_105at 151.173 kb on + strand, within Pf6N2E2_105at 151.173 kb on + strand, within Pf6N2E2_105at 151.174 kb on - strand, within Pf6N2E2_105at 151.174 kb on - strand, within Pf6N2E2_105at 151.174 kb on - strand, within Pf6N2E2_105at 151.258 kb on - strand, within Pf6N2E2_105at 151.287 kb on + strand, within Pf6N2E2_105at 151.288 kb on - strand, within Pf6N2E2_105at 151.288 kb on - strand, within Pf6N2E2_105at 151.290 kb on + strand, within Pf6N2E2_105at 151.290 kb on + strandat 151.290 kb on + strand, within Pf6N2E2_105at 151.291 kb on - strand, within Pf6N2E2_105at 151.413 kb on + strand, within Pf6N2E2_105at 151.414 kb on - strand, within Pf6N2E2_105at 151.540 kb on - strandat 151.540 kb on - strandat 151.540 kb on - strandat 151.540 kb on - strandat 151.575 kb on + strandat 151.575 kb on + strandat 151.654 kb on + strandat 151.655 kb on - strandat 151.655 kb on - strandat 151.655 kb on - strandat 151.681 kb on + strandat 151.695 kb on + strandat 151.695 kb on + strandat 151.696 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Mannose (C)
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148,652 - +1.2
149,037 - Pf6N2E2_103 0.45 +0.3
149,037 - Pf6N2E2_103 0.45 -1.2
149,204 + Pf6N2E2_103 0.60 +1.4
149,383 + Pf6N2E2_103 0.77 +0.5
149,383 + Pf6N2E2_103 0.77 -0.3
149,384 - Pf6N2E2_103 0.77 +1.1
149,384 - Pf6N2E2_103 0.77 +0.7
149,525 + Pf6N2E2_103 0.90 -1.4
149,525 + Pf6N2E2_103 0.90 +0.7
149,552 + -1.6
149,552 + -1.4
149,553 - +1.1
149,675 - -1.4
149,675 - -0.0
149,914 + Pf6N2E2_104 0.25 -0.6
149,914 + Pf6N2E2_104 0.25 -0.3
149,914 + Pf6N2E2_104 0.25 -0.8
149,915 - Pf6N2E2_104 0.25 +1.1
149,915 - Pf6N2E2_104 0.25 -0.3
149,915 - Pf6N2E2_104 0.25 -0.2
149,942 + Pf6N2E2_104 0.27 -0.4
149,943 - Pf6N2E2_104 0.27 -0.7
149,943 - Pf6N2E2_104 0.27 +0.4
150,223 + Pf6N2E2_104 0.53 -0.3
150,224 - Pf6N2E2_104 0.53 -0.8
150,280 + Pf6N2E2_104 0.58 -0.4
150,281 - Pf6N2E2_104 0.58 +1.1
150,281 - Pf6N2E2_104 0.58 +0.8
150,532 + Pf6N2E2_104 0.81 -0.1
150,533 - Pf6N2E2_104 0.81 -0.6
150,583 + Pf6N2E2_104 0.85 -0.4
150,583 + Pf6N2E2_104 0.85 -0.1
150,584 - Pf6N2E2_104 0.85 -0.6
150,584 - Pf6N2E2_104 0.85 -0.7
150,627 - Pf6N2E2_104 0.89 -0.5
150,667 + -2.2
150,667 + +0.2
150,667 + -0.3
150,701 - -1.1
150,712 + -1.8
150,712 + +0.5
150,879 + Pf6N2E2_105 0.16 +0.1
151,167 + Pf6N2E2_105 0.49 -0.5
151,167 + Pf6N2E2_105 0.49 +0.1
151,168 - Pf6N2E2_105 0.49 -2.3
151,168 - Pf6N2E2_105 0.49 +1.5
151,173 + Pf6N2E2_105 0.49 -0.1
151,173 + Pf6N2E2_105 0.49 +0.7
151,174 - Pf6N2E2_105 0.50 +0.8
151,174 - Pf6N2E2_105 0.50 -0.8
151,174 - Pf6N2E2_105 0.50 +0.8
151,258 - Pf6N2E2_105 0.59 +0.5
151,287 + Pf6N2E2_105 0.63 +0.7
151,288 - Pf6N2E2_105 0.63 -0.9
151,288 - Pf6N2E2_105 0.63 +0.1
151,290 + Pf6N2E2_105 0.63 -0.8
151,290 + +2.1
151,290 + Pf6N2E2_105 0.63 +1.4
151,291 - Pf6N2E2_105 0.63 +0.2
151,413 + Pf6N2E2_105 0.77 +0.1
151,414 - Pf6N2E2_105 0.77 +0.5
151,540 - -1.1
151,540 - +0.8
151,540 - +0.6
151,540 - -1.5
151,575 + -0.6
151,575 + +0.5
151,654 + +0.4
151,655 - +0.4
151,655 - -0.9
151,655 - -0.5
151,681 + -0.3
151,695 + +0.7
151,695 + +0.4
151,696 - -0.6

Or see this region's nucleotide sequence