Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04865 and MMP_RS04870 are separated by 15 nucleotides MMP_RS04870 and MMP_RS04875 are separated by 22 nucleotides MMP_RS04875 and MMP_RS04880 are separated by 64 nucleotides
MMP_RS04865: MMP_RS04865 - adenosylcobinamide-GDP ribazoletransferase, at 925,590 to 926,363
_RS04865
MMP_RS04870: MMP_RS04870 - TIGR02253 family HAD-type hydrolase, at 926,379 to 927,056
_RS04870
MMP_RS04875: MMP_RS04875 - energy-converting hydrogenase B subunit P, at 927,079 to 927,366
_RS04875
MMP_RS04880: MMP_RS04880 - phosphoadenosine phosphosulfate reductase family protein, at 927,431 to 928,942
_RS04880
Position (kb) 927
928 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 926.088 kb on - strand at 926.145 kb on + strand at 926.145 kb on + strand at 926.147 kb on + strand at 926.148 kb on - strand at 926.213 kb on - strand at 926.359 kb on + strand at 926.384 kb on - strand at 926.385 kb on + strand at 926.393 kb on - strand at 926.417 kb on - strand at 926.431 kb on - strand at 926.432 kb on + strand at 926.440 kb on + strand at 926.440 kb on + strand at 926.440 kb on + strand at 926.517 kb on + strand, within MMP_RS04870 at 926.568 kb on + strand, within MMP_RS04870 at 926.589 kb on + strand, within MMP_RS04870 at 926.620 kb on - strand, within MMP_RS04870 at 926.621 kb on + strand, within MMP_RS04870 at 926.635 kb on + strand, within MMP_RS04870 at 926.636 kb on - strand, within MMP_RS04870 at 926.637 kb on + strand, within MMP_RS04870 at 926.808 kb on + strand, within MMP_RS04870 at 926.912 kb on - strand, within MMP_RS04870 at 927.073 kb on - strand at 927.073 kb on - strand at 927.073 kb on - strand at 927.074 kb on + strand at 927.076 kb on + strand at 927.083 kb on + strand at 927.118 kb on + strand, within MMP_RS04875 at 927.158 kb on + strand, within MMP_RS04875 at 927.294 kb on + strand, within MMP_RS04875 at 927.313 kb on - strand, within MMP_RS04875 at 927.314 kb on + strand, within MMP_RS04875 at 927.317 kb on - strand, within MMP_RS04875 at 927.318 kb on + strand, within MMP_RS04875 at 927.318 kb on + strand, within MMP_RS04875 at 927.352 kb on - strand at 927.353 kb on + strand at 927.367 kb on - strand at 927.367 kb on - strand at 927.368 kb on + strand at 927.368 kb on + strand at 927.368 kb on + strand at 927.369 kb on - strand at 927.369 kb on - strand at 927.369 kb on - strand at 927.370 kb on + strand at 927.370 kb on + strand at 927.370 kb on + strand at 927.386 kb on - strand at 927.392 kb on - strand at 927.393 kb on + strand at 927.397 kb on - strand at 927.398 kb on + strand at 927.398 kb on + strand at 927.398 kb on + strand at 927.411 kb on - strand at 927.411 kb on - strand at 927.411 kb on - strand at 927.412 kb on + strand at 927.412 kb on + strand at 927.412 kb on + strand at 927.412 kb on + strand at 927.412 kb on + strand at 927.412 kb on + strand at 927.413 kb on - strand at 927.413 kb on - strand at 927.413 kb on - strand at 927.414 kb on + strand at 927.414 kb on + strand at 927.414 kb on + strand at 927.414 kb on + strand at 927.425 kb on - strand at 927.606 kb on + strand, within MMP_RS04880 at 927.675 kb on + strand, within MMP_RS04880 at 927.678 kb on + strand, within MMP_RS04880 at 927.695 kb on + strand, within MMP_RS04880 at 927.895 kb on - strand, within MMP_RS04880 at 927.899 kb on - strand, within MMP_RS04880 at 927.996 kb on - strand, within MMP_RS04880 at 927.996 kb on - strand, within MMP_RS04880 at 928.000 kb on - strand, within MMP_RS04880 at 928.001 kb on + strand, within MMP_RS04880 at 928.001 kb on + strand, within MMP_RS04880 at 928.001 kb on + strand, within MMP_RS04880 at 928.001 kb on + strand, within MMP_RS04880 at 928.001 kb on + strand, within MMP_RS04880 at 928.112 kb on - strand, within MMP_RS04880
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE remove 926,088 - -3.3 926,145 + -3.6 926,145 + -1.0 926,147 + -4.0 926,148 - -1.8 926,213 - -2.7 926,359 + -2.9 926,384 - -1.9 926,385 + -2.4 926,393 - -0.9 926,417 - -0.3 926,431 - -1.8 926,432 + -2.8 926,440 + -3.8 926,440 + -1.8 926,440 + -1.7 926,517 + MMP_RS04870 0.20 -3.7 926,568 + MMP_RS04870 0.28 -3.1 926,589 + MMP_RS04870 0.31 -4.2 926,620 - MMP_RS04870 0.36 -1.3 926,621 + MMP_RS04870 0.36 -2.0 926,635 + MMP_RS04870 0.38 -2.6 926,636 - MMP_RS04870 0.38 -1.6 926,637 + MMP_RS04870 0.38 -3.9 926,808 + MMP_RS04870 0.63 -3.7 926,912 - MMP_RS04870 0.79 -1.3 927,073 - +0.6 927,073 - -1.4 927,073 - -2.2 927,074 + -2.7 927,076 + -2.2 927,083 + -1.0 927,118 + MMP_RS04875 0.14 -2.9 927,158 + MMP_RS04875 0.27 -3.1 927,294 + MMP_RS04875 0.75 -2.6 927,313 - MMP_RS04875 0.81 -1.4 927,314 + MMP_RS04875 0.82 -3.3 927,317 - MMP_RS04875 0.83 -1.4 927,318 + MMP_RS04875 0.83 -0.8 927,318 + MMP_RS04875 0.83 -2.0 927,352 - -1.3 927,353 + -2.6 927,367 - -2.3 927,367 - -1.5 927,368 + -2.4 927,368 + +0.3 927,368 + -2.3 927,369 - -1.1 927,369 - -1.0 927,369 - -1.1 927,370 + -2.6 927,370 + -2.4 927,370 + -3.0 927,386 - -2.0 927,392 - -0.6 927,393 + -3.8 927,397 - -3.1 927,398 + -2.5 927,398 + -2.9 927,398 + -2.6 927,411 - -0.2 927,411 - -0.6 927,411 - -0.8 927,412 + -0.4 927,412 + -0.8 927,412 + -0.3 927,412 + -2.2 927,412 + -1.3 927,412 + -1.1 927,413 - -1.0 927,413 - -0.4 927,413 - -0.0 927,414 + -0.3 927,414 + -0.6 927,414 + -0.4 927,414 + +0.3 927,425 - +0.5 927,606 + MMP_RS04880 0.12 -0.4 927,675 + MMP_RS04880 0.16 +0.2 927,678 + MMP_RS04880 0.16 -0.1 927,695 + MMP_RS04880 0.17 +0.7 927,895 - MMP_RS04880 0.31 -0.3 927,899 - MMP_RS04880 0.31 -0.7 927,996 - MMP_RS04880 0.37 +0.2 927,996 - MMP_RS04880 0.37 -0.2 928,000 - MMP_RS04880 0.38 -0.2 928,001 + MMP_RS04880 0.38 -1.0 928,001 + MMP_RS04880 0.38 +0.5 928,001 + MMP_RS04880 0.38 -0.2 928,001 + MMP_RS04880 0.38 -0.4 928,001 + MMP_RS04880 0.38 -0.8 928,112 - MMP_RS04880 0.45 -0.5
Or see this region's nucleotide sequence