Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01465 and MMP_RS01470 are separated by 25 nucleotides MMP_RS01470 and MMP_RS01475 are separated by 18 nucleotides MMP_RS01475 and MMP_RS01480 are separated by 211 nucleotides
MMP_RS01465: MMP_RS01465 - hypothetical protein, at 270,743 to 271,441
_RS01465
MMP_RS01470: MMP_RS01470 - ATP-binding cassette domain-containing protein, at 271,467 to 272,258
_RS01470
MMP_RS01475: MMP_RS01475 - phosphosulfolactate synthase, at 272,277 to 273,032
_RS01475
MMP_RS01480: MMP_RS01480 - hydrogenase expression/formation protein HypE, at 273,244 to 274,248
_RS01480
Position (kb) 272
273
274 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 271.396 kb on - strand at 271.686 kb on - strand, within MMP_RS01470 at 271.767 kb on - strand, within MMP_RS01470 at 271.839 kb on - strand, within MMP_RS01470 at 271.890 kb on - strand, within MMP_RS01470 at 271.974 kb on + strand, within MMP_RS01470 at 272.187 kb on - strand at 272.382 kb on - strand, within MMP_RS01475 at 272.388 kb on + strand, within MMP_RS01475 at 272.427 kb on - strand, within MMP_RS01475 at 272.428 kb on + strand, within MMP_RS01475 at 272.428 kb on + strand, within MMP_RS01475 at 272.750 kb on - strand, within MMP_RS01475 at 272.764 kb on - strand, within MMP_RS01475 at 272.794 kb on + strand, within MMP_RS01475 at 272.794 kb on + strand, within MMP_RS01475 at 272.819 kb on + strand, within MMP_RS01475 at 272.819 kb on + strand, within MMP_RS01475 at 272.931 kb on - strand, within MMP_RS01475 at 272.937 kb on - strand, within MMP_RS01475 at 273.052 kb on + strand at 273.102 kb on - strand at 273.103 kb on + strand at 273.105 kb on + strand at 273.119 kb on + strand at 273.121 kb on + strand at 273.122 kb on - strand at 273.210 kb on - strand at 273.225 kb on - strand at 273.293 kb on - strand at 273.294 kb on + strand at 273.423 kb on - strand at 273.684 kb on + strand at 273.965 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE remove 271,396 - +0.6 271,686 - MMP_RS01470 0.28 -0.3 271,767 - MMP_RS01470 0.38 +0.6 271,839 - MMP_RS01470 0.47 +0.3 271,890 - MMP_RS01470 0.53 -1.1 271,974 + MMP_RS01470 0.64 -0.9 272,187 - -0.2 272,382 - MMP_RS01475 0.14 -1.4 272,388 + MMP_RS01475 0.15 -1.7 272,427 - MMP_RS01475 0.20 -2.0 272,428 + MMP_RS01475 0.20 -1.9 272,428 + MMP_RS01475 0.20 -1.8 272,750 - MMP_RS01475 0.63 -0.9 272,764 - MMP_RS01475 0.64 -1.3 272,794 + MMP_RS01475 0.68 -1.8 272,794 + MMP_RS01475 0.68 -1.3 272,819 + MMP_RS01475 0.72 -1.1 272,819 + MMP_RS01475 0.72 -2.1 272,931 - MMP_RS01475 0.87 -2.0 272,937 - MMP_RS01475 0.87 -1.1 273,052 + -2.6 273,102 - -1.3 273,103 + -1.5 273,105 + -0.4 273,119 + -0.7 273,121 + -1.0 273,122 - +0.8 273,210 - -3.3 273,225 - -2.5 273,293 - -3.6 273,294 + -4.5 273,423 - -2.3 273,684 + -4.0 273,965 - -3.6
Or see this region's nucleotide sequence