Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01245 and MMP_RS01250 are separated by 230 nucleotides MMP_RS01250 and MMP_RS01255 are separated by 33 nucleotides MMP_RS01255 and MMP_RS01260 are separated by 40 nucleotides
MMP_RS01245: MMP_RS01245 - anaerobic ribonucleoside-triphosphate reductase, at 234,309 to 236,621
_RS01245
MMP_RS01250: MMP_RS01250 - tRNA (guanine(26)-N(2))-dimethyltransferase, at 236,852 to 237,973
_RS01250
MMP_RS01255: MMP_RS01255 - amino acid ABC transporter ATP-binding protein, at 238,007 to 238,738
_RS01255
MMP_RS01260: MMP_RS01260 - HDIG domain-containing protein, at 238,779 to 239,465
_RS01260
Position (kb) 236
237
238 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 236.434 kb on + strand at 236.493 kb on - strand at 236.602 kb on - strand at 236.703 kb on + strand at 236.717 kb on - strand at 236.730 kb on + strand at 236.732 kb on + strand at 236.747 kb on + strand at 236.747 kb on + strand at 236.752 kb on + strand at 236.752 kb on + strand at 236.756 kb on + strand at 236.756 kb on + strand at 236.764 kb on - strand at 236.764 kb on - strand at 236.766 kb on - strand at 236.766 kb on - strand at 236.766 kb on - strand at 236.766 kb on - strand at 236.767 kb on + strand at 236.767 kb on + strand at 236.768 kb on - strand at 236.768 kb on - strand at 236.769 kb on + strand at 236.769 kb on + strand at 236.805 kb on + strand at 236.809 kb on + strand at 236.860 kb on + strand at 236.863 kb on - strand at 236.920 kb on - strand at 237.222 kb on - strand, within MMP_RS01250 at 237.343 kb on + strand, within MMP_RS01250 at 237.572 kb on - strand, within MMP_RS01250 at 237.605 kb on + strand, within MMP_RS01250 at 237.872 kb on + strand at 237.877 kb on - strand at 237.983 kb on + strand at 237.989 kb on - strand at 237.993 kb on - strand at 237.994 kb on + strand at 237.994 kb on + strand at 238.009 kb on + strand at 238.066 kb on - strand at 238.067 kb on + strand at 238.205 kb on - strand, within MMP_RS01255 at 238.215 kb on + strand, within MMP_RS01255 at 238.262 kb on - strand, within MMP_RS01255 at 238.450 kb on - strand, within MMP_RS01255 at 238.646 kb on - strand, within MMP_RS01255
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE remove 236,434 + -2.6 236,493 - -3.8 236,602 - -2.9 236,703 + -2.0 236,717 - -3.7 236,730 + -2.7 236,732 + -2.6 236,747 + -2.3 236,747 + -1.2 236,752 + -2.9 236,752 + -3.3 236,756 + -0.9 236,756 + -1.3 236,764 - -1.2 236,764 - -2.9 236,766 - -1.1 236,766 - -1.5 236,766 - -1.8 236,766 - -1.8 236,767 + -1.8 236,767 + -2.0 236,768 - -0.7 236,768 - -0.5 236,769 + -0.3 236,769 + +0.3 236,805 + -0.5 236,809 + -2.5 236,860 + -3.4 236,863 - -2.2 236,920 - -3.0 237,222 - MMP_RS01250 0.33 -2.5 237,343 + MMP_RS01250 0.44 -3.3 237,572 - MMP_RS01250 0.64 -2.6 237,605 + MMP_RS01250 0.67 -4.1 237,872 + -3.7 237,877 - -3.7 237,983 + -0.3 237,989 - +1.0 237,993 - +0.0 237,994 + +0.3 237,994 + +0.2 238,009 + +0.6 238,066 - +0.0 238,067 + -0.5 238,205 - MMP_RS01255 0.27 +0.4 238,215 + MMP_RS01255 0.28 +0.1 238,262 - MMP_RS01255 0.35 +0.1 238,450 - MMP_RS01255 0.61 +0.1 238,646 - MMP_RS01255 0.87 +0.0
Or see this region's nucleotide sequence