Strain Fitness in Methanococcus maripaludis S2 around MMP_RS01245

Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS01235 and MMP_RS01240 are separated by 15 nucleotidesMMP_RS01240 and MMP_RS01245 are separated by 131 nucleotidesMMP_RS01245 and MMP_RS01250 are separated by 230 nucleotides MMP_RS01235: MMP_RS01235 - iron-containing alcohol dehydrogenase, at 232,456 to 233,460 _RS01235 MMP_RS01240: MMP_RS01240 - 7-cyano-7-deazaguanine synthase QueC, at 233,476 to 234,177 _RS01240 MMP_RS01245: MMP_RS01245 - anaerobic ribonucleoside-triphosphate reductase, at 234,309 to 236,621 _RS01245 MMP_RS01250: MMP_RS01250 - tRNA (guanine(26)-N(2))-dimethyltransferase, at 236,852 to 237,973 _RS01250 Position (kb) 234 235 236 237Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 233.312 kb on - strandat 233.340 kb on + strandat 234.087 kb on - strand, within MMP_RS01240at 234.094 kb on + strand, within MMP_RS01240at 234.156 kb on - strandat 234.214 kb on + strandat 234.285 kb on + strandat 234.333 kb on + strandat 234.509 kb on + strandat 235.725 kb on - strandat 236.434 kb on + strandat 236.493 kb on - strandat 236.602 kb on - strandat 236.703 kb on + strandat 236.717 kb on - strandat 236.730 kb on + strandat 236.732 kb on + strandat 236.747 kb on + strandat 236.747 kb on + strandat 236.752 kb on + strandat 236.752 kb on + strandat 236.756 kb on + strandat 236.756 kb on + strandat 236.764 kb on - strandat 236.764 kb on - strandat 236.766 kb on - strandat 236.766 kb on - strandat 236.766 kb on - strandat 236.766 kb on - strandat 236.767 kb on + strandat 236.767 kb on + strandat 236.768 kb on - strandat 236.768 kb on - strandat 236.769 kb on + strandat 236.769 kb on + strandat 236.805 kb on + strandat 236.809 kb on + strandat 236.860 kb on + strandat 236.863 kb on - strandat 236.920 kb on - strandat 237.222 kb on - strand, within MMP_RS01250at 237.343 kb on + strand, within MMP_RS01250at 237.572 kb on - strand, within MMP_RS01250at 237.605 kb on + strand, within MMP_RS01250

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Per-strain Table

Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE
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233,312 - -0.8
233,340 + -3.5
234,087 - MMP_RS01240 0.87 +0.0
234,094 + MMP_RS01240 0.88 -3.0
234,156 - -0.2
234,214 + -4.8
234,285 + -0.8
234,333 + +0.6
234,509 + -3.8
235,725 - -3.4
236,434 + -2.6
236,493 - -3.8
236,602 - -2.9
236,703 + -2.0
236,717 - -3.7
236,730 + -2.7
236,732 + -2.6
236,747 + -2.3
236,747 + -1.2
236,752 + -2.9
236,752 + -3.3
236,756 + -0.9
236,756 + -1.3
236,764 - -1.2
236,764 - -2.9
236,766 - -1.1
236,766 - -1.5
236,766 - -1.8
236,766 - -1.8
236,767 + -1.8
236,767 + -2.0
236,768 - -0.7
236,768 - -0.5
236,769 + -0.3
236,769 + +0.3
236,805 + -0.5
236,809 + -2.5
236,860 + -3.4
236,863 - -2.2
236,920 - -3.0
237,222 - MMP_RS01250 0.33 -2.5
237,343 + MMP_RS01250 0.44 -3.3
237,572 - MMP_RS01250 0.64 -2.6
237,605 + MMP_RS01250 0.67 -4.1

Or see this region's nucleotide sequence