Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS25350 and CA265_RS25355 are separated by 114 nucleotides CA265_RS25355 and CA265_RS25360 are separated by 194 nucleotides CA265_RS25360 and CA265_RS25365 are separated by 229 nucleotides
CA265_RS25350: CA265_RS25350 - serine hydrolase, at 6,161,372 to 6,162,688
_RS25350
CA265_RS25355: CA265_RS25355 - DUF2807 domain-containing protein, at 6,162,803 to 6,163,507
_RS25355
CA265_RS25360: CA265_RS25360 - phosphoribosyltransferase, at 6,163,702 to 6,164,208
_RS25360
CA265_RS25365: CA265_RS25365 - glycerol acyltransferase, at 6,164,438 to 6,165,061
_RS25365
Position (kb)
6162
6163
6164 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6161.809 kb on - strand, within CA265_RS25350 at 6161.830 kb on + strand, within CA265_RS25350 at 6161.838 kb on - strand, within CA265_RS25350 at 6161.838 kb on - strand, within CA265_RS25350 at 6161.956 kb on + strand, within CA265_RS25350 at 6161.957 kb on - strand, within CA265_RS25350 at 6162.002 kb on - strand, within CA265_RS25350 at 6162.017 kb on - strand, within CA265_RS25350 at 6162.017 kb on - strand, within CA265_RS25350 at 6162.018 kb on + strand, within CA265_RS25350 at 6162.055 kb on + strand, within CA265_RS25350 at 6162.058 kb on - strand, within CA265_RS25350 at 6162.235 kb on + strand, within CA265_RS25350 at 6162.235 kb on + strand, within CA265_RS25350 at 6162.236 kb on - strand, within CA265_RS25350 at 6162.260 kb on - strand, within CA265_RS25350 at 6162.311 kb on - strand, within CA265_RS25350 at 6162.311 kb on - strand, within CA265_RS25350 at 6162.311 kb on - strand, within CA265_RS25350 at 6162.386 kb on + strand, within CA265_RS25350 at 6162.416 kb on - strand, within CA265_RS25350 at 6162.469 kb on + strand, within CA265_RS25350 at 6162.480 kb on + strand, within CA265_RS25350 at 6162.480 kb on + strand, within CA265_RS25350 at 6162.524 kb on + strand, within CA265_RS25350 at 6162.524 kb on + strand, within CA265_RS25350 at 6162.525 kb on - strand, within CA265_RS25350 at 6162.547 kb on + strand, within CA265_RS25350 at 6162.552 kb on - strand, within CA265_RS25350 at 6162.556 kb on + strand, within CA265_RS25350 at 6162.557 kb on - strand at 6162.589 kb on + strand at 6162.595 kb on - strand at 6162.707 kb on + strand at 6162.723 kb on + strand at 6162.738 kb on + strand at 6162.742 kb on + strand at 6162.784 kb on + strand at 6162.876 kb on - strand, within CA265_RS25355 at 6162.917 kb on + strand, within CA265_RS25355 at 6162.917 kb on + strand, within CA265_RS25355 at 6162.929 kb on + strand, within CA265_RS25355 at 6163.021 kb on + strand, within CA265_RS25355 at 6163.033 kb on + strand, within CA265_RS25355 at 6163.104 kb on - strand, within CA265_RS25355 at 6163.139 kb on - strand, within CA265_RS25355 at 6163.214 kb on + strand, within CA265_RS25355 at 6163.215 kb on - strand, within CA265_RS25355 at 6163.215 kb on - strand, within CA265_RS25355 at 6163.240 kb on + strand, within CA265_RS25355 at 6163.313 kb on - strand, within CA265_RS25355 at 6163.341 kb on - strand, within CA265_RS25355 at 6163.343 kb on - strand, within CA265_RS25355 at 6163.395 kb on - strand, within CA265_RS25355 at 6163.406 kb on - strand, within CA265_RS25355 at 6163.406 kb on - strand, within CA265_RS25355 at 6163.455 kb on + strand at 6163.456 kb on - strand at 6163.472 kb on - strand at 6163.698 kb on + strand at 6163.715 kb on + strand at 6163.716 kb on - strand at 6163.716 kb on - strand at 6163.719 kb on + strand at 6163.719 kb on + strand at 6163.845 kb on - strand, within CA265_RS25360 at 6163.864 kb on - strand, within CA265_RS25360 at 6163.969 kb on - strand, within CA265_RS25360 at 6163.998 kb on - strand, within CA265_RS25360 at 6163.998 kb on - strand, within CA265_RS25360 at 6164.044 kb on - strand, within CA265_RS25360 at 6164.053 kb on - strand, within CA265_RS25360 at 6164.070 kb on - strand, within CA265_RS25360 at 6164.175 kb on - strand at 6164.231 kb on + strand at 6164.231 kb on + strand at 6164.232 kb on - strand at 6164.232 kb on - strand at 6164.247 kb on - strand at 6164.250 kb on - strand at 6164.303 kb on + strand at 6164.303 kb on + strand at 6164.306 kb on - strand at 6164.309 kb on + strand at 6164.309 kb on + strand at 6164.310 kb on - strand at 6164.388 kb on + strand at 6164.400 kb on + strand at 6164.400 kb on + strand at 6164.486 kb on + strand at 6164.498 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 6,161,809 - CA265_RS25350 0.33 -0.2 6,161,830 + CA265_RS25350 0.35 +1.3 6,161,838 - CA265_RS25350 0.35 -0.0 6,161,838 - CA265_RS25350 0.35 -1.6 6,161,956 + CA265_RS25350 0.44 +1.1 6,161,957 - CA265_RS25350 0.44 -0.0 6,162,002 - CA265_RS25350 0.48 -0.7 6,162,017 - CA265_RS25350 0.49 +1.4 6,162,017 - CA265_RS25350 0.49 -1.3 6,162,018 + CA265_RS25350 0.49 -0.2 6,162,055 + CA265_RS25350 0.52 +0.2 6,162,058 - CA265_RS25350 0.52 +1.0 6,162,235 + CA265_RS25350 0.66 -0.7 6,162,235 + CA265_RS25350 0.66 +0.3 6,162,236 - CA265_RS25350 0.66 +2.0 6,162,260 - CA265_RS25350 0.67 +0.2 6,162,311 - CA265_RS25350 0.71 +0.3 6,162,311 - CA265_RS25350 0.71 -0.1 6,162,311 - CA265_RS25350 0.71 -0.1 6,162,386 + CA265_RS25350 0.77 -0.1 6,162,416 - CA265_RS25350 0.79 -0.5 6,162,469 + CA265_RS25350 0.83 -0.5 6,162,480 + CA265_RS25350 0.84 -0.4 6,162,480 + CA265_RS25350 0.84 +1.5 6,162,524 + CA265_RS25350 0.87 -1.2 6,162,524 + CA265_RS25350 0.87 -0.9 6,162,525 - CA265_RS25350 0.88 +0.1 6,162,547 + CA265_RS25350 0.89 +0.5 6,162,552 - CA265_RS25350 0.90 +0.5 6,162,556 + CA265_RS25350 0.90 +0.3 6,162,557 - -0.7 6,162,589 + -0.3 6,162,595 - -0.3 6,162,707 + -0.2 6,162,723 + +0.0 6,162,738 + -0.7 6,162,742 + +0.1 6,162,784 + -0.6 6,162,876 - CA265_RS25355 0.10 +2.1 6,162,917 + CA265_RS25355 0.16 -0.2 6,162,917 + CA265_RS25355 0.16 -1.7 6,162,929 + CA265_RS25355 0.18 +0.4 6,163,021 + CA265_RS25355 0.31 -0.5 6,163,033 + CA265_RS25355 0.33 +0.0 6,163,104 - CA265_RS25355 0.43 +3.5 6,163,139 - CA265_RS25355 0.48 +2.4 6,163,214 + CA265_RS25355 0.58 +0.2 6,163,215 - CA265_RS25355 0.58 +1.5 6,163,215 - CA265_RS25355 0.58 +2.8 6,163,240 + CA265_RS25355 0.62 -0.0 6,163,313 - CA265_RS25355 0.72 +3.2 6,163,341 - CA265_RS25355 0.76 +2.0 6,163,343 - CA265_RS25355 0.77 +2.6 6,163,395 - CA265_RS25355 0.84 +3.4 6,163,406 - CA265_RS25355 0.86 +2.7 6,163,406 - CA265_RS25355 0.86 +3.2 6,163,455 + +0.8 6,163,456 - +2.8 6,163,472 - +2.0 6,163,698 + -1.3 6,163,715 + -1.4 6,163,716 - +3.3 6,163,716 - +3.7 6,163,719 + -0.8 6,163,719 + +0.1 6,163,845 - CA265_RS25360 0.28 +1.3 6,163,864 - CA265_RS25360 0.32 +2.7 6,163,969 - CA265_RS25360 0.53 +0.8 6,163,998 - CA265_RS25360 0.58 +0.1 6,163,998 - CA265_RS25360 0.58 -1.1 6,164,044 - CA265_RS25360 0.67 -1.2 6,164,053 - CA265_RS25360 0.69 -0.8 6,164,070 - CA265_RS25360 0.73 +0.5 6,164,175 - -2.8 6,164,231 + +0.4 6,164,231 + +2.3 6,164,232 - -1.0 6,164,232 - -0.4 6,164,247 - -0.3 6,164,250 - -0.7 6,164,303 + -0.2 6,164,303 + -0.2 6,164,306 - -1.1 6,164,309 + +0.4 6,164,309 + -0.3 6,164,310 - -1.0 6,164,388 + +0.1 6,164,400 + -0.5 6,164,400 + -0.3 6,164,486 + -0.6 6,164,498 - -0.9
Or see this region's nucleotide sequence