Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS23900 and CA265_RS23905 are separated by 119 nucleotides CA265_RS23905 and CA265_RS23910 are separated by 254 nucleotides
CA265_RS23900: CA265_RS23900 - N-acyl-L-amino acid amidohydrolase, at 5,829,495 to 5,830,679
_RS23900
CA265_RS23905: CA265_RS23905 - hypothetical protein, at 5,830,799 to 5,831,740
_RS23905
CA265_RS23910: CA265_RS23910 - serine hydrolase, at 5,831,995 to 5,833,563
_RS23910
Position (kb) 5830
5831
5832 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5830.677 kb on + strand at 5830.740 kb on + strand at 5830.740 kb on + strand at 5830.741 kb on - strand at 5830.741 kb on - strand at 5830.741 kb on - strand at 5830.741 kb on - strand at 5830.745 kb on - strand at 5830.830 kb on + strand at 5830.897 kb on + strand, within CA265_RS23905 at 5830.921 kb on + strand, within CA265_RS23905 at 5830.922 kb on - strand, within CA265_RS23905 at 5830.967 kb on + strand, within CA265_RS23905 at 5830.983 kb on - strand, within CA265_RS23905 at 5831.011 kb on + strand, within CA265_RS23905 at 5831.036 kb on + strand, within CA265_RS23905 at 5831.036 kb on + strand, within CA265_RS23905 at 5831.036 kb on + strand, within CA265_RS23905 at 5831.038 kb on + strand, within CA265_RS23905 at 5831.150 kb on + strand, within CA265_RS23905 at 5831.168 kb on + strand, within CA265_RS23905 at 5831.177 kb on + strand, within CA265_RS23905 at 5831.178 kb on - strand, within CA265_RS23905 at 5831.281 kb on + strand, within CA265_RS23905 at 5831.348 kb on - strand, within CA265_RS23905 at 5831.372 kb on - strand, within CA265_RS23905 at 5831.382 kb on - strand, within CA265_RS23905 at 5831.390 kb on + strand, within CA265_RS23905 at 5831.390 kb on + strand, within CA265_RS23905 at 5831.411 kb on + strand, within CA265_RS23905 at 5831.422 kb on + strand, within CA265_RS23905 at 5831.464 kb on - strand, within CA265_RS23905 at 5831.464 kb on - strand, within CA265_RS23905 at 5831.570 kb on - strand, within CA265_RS23905 at 5831.590 kb on - strand, within CA265_RS23905 at 5831.602 kb on - strand, within CA265_RS23905 at 5831.644 kb on + strand, within CA265_RS23905 at 5831.649 kb on - strand at 5831.656 kb on + strand at 5831.706 kb on + strand at 5831.706 kb on + strand at 5831.707 kb on - strand at 5831.710 kb on + strand at 5831.711 kb on - strand at 5831.738 kb on + strand at 5831.790 kb on + strand at 5831.799 kb on + strand at 5831.804 kb on - strand at 5831.804 kb on - strand at 5831.815 kb on - strand at 5831.815 kb on - strand at 5831.840 kb on + strand at 5831.841 kb on - strand at 5831.841 kb on - strand at 5831.848 kb on - strand at 5831.873 kb on - strand at 5831.965 kb on - strand at 5832.075 kb on - strand at 5832.105 kb on - strand at 5832.180 kb on - strand, within CA265_RS23910 at 5832.194 kb on + strand, within CA265_RS23910 at 5832.195 kb on - strand, within CA265_RS23910 at 5832.231 kb on + strand, within CA265_RS23910 at 5832.309 kb on + strand, within CA265_RS23910 at 5832.349 kb on - strand, within CA265_RS23910 at 5832.356 kb on - strand, within CA265_RS23910 at 5832.368 kb on + strand, within CA265_RS23910 at 5832.382 kb on - strand, within CA265_RS23910 at 5832.399 kb on + strand, within CA265_RS23910 at 5832.399 kb on + strand, within CA265_RS23910 at 5832.437 kb on + strand, within CA265_RS23910 at 5832.530 kb on - strand, within CA265_RS23910 at 5832.573 kb on - strand, within CA265_RS23910 at 5832.592 kb on - strand, within CA265_RS23910 at 5832.596 kb on - strand, within CA265_RS23910 at 5832.625 kb on - strand, within CA265_RS23910 at 5832.636 kb on + strand, within CA265_RS23910 at 5832.636 kb on + strand, within CA265_RS23910 at 5832.637 kb on - strand, within CA265_RS23910 at 5832.637 kb on - strand, within CA265_RS23910
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 5,830,677 + -0.5 5,830,740 + +0.5 5,830,740 + +2.0 5,830,741 - -0.7 5,830,741 - -1.3 5,830,741 - +0.5 5,830,741 - -0.4 5,830,745 - +0.0 5,830,830 + -2.4 5,830,897 + CA265_RS23905 0.10 -1.8 5,830,921 + CA265_RS23905 0.13 -1.6 5,830,922 - CA265_RS23905 0.13 -0.9 5,830,967 + CA265_RS23905 0.18 -2.2 5,830,983 - CA265_RS23905 0.20 -1.6 5,831,011 + CA265_RS23905 0.23 -2.9 5,831,036 + CA265_RS23905 0.25 -2.4 5,831,036 + CA265_RS23905 0.25 -2.3 5,831,036 + CA265_RS23905 0.25 -3.1 5,831,038 + CA265_RS23905 0.25 -2.9 5,831,150 + CA265_RS23905 0.37 -2.4 5,831,168 + CA265_RS23905 0.39 +0.9 5,831,177 + CA265_RS23905 0.40 -1.0 5,831,178 - CA265_RS23905 0.40 -2.1 5,831,281 + CA265_RS23905 0.51 -3.0 5,831,348 - CA265_RS23905 0.58 -1.8 5,831,372 - CA265_RS23905 0.61 -1.1 5,831,382 - CA265_RS23905 0.62 -1.7 5,831,390 + CA265_RS23905 0.63 -1.8 5,831,390 + CA265_RS23905 0.63 -0.7 5,831,411 + CA265_RS23905 0.65 -2.4 5,831,422 + CA265_RS23905 0.66 -2.8 5,831,464 - CA265_RS23905 0.71 -0.6 5,831,464 - CA265_RS23905 0.71 -0.6 5,831,570 - CA265_RS23905 0.82 -1.2 5,831,590 - CA265_RS23905 0.84 -1.4 5,831,602 - CA265_RS23905 0.85 -3.0 5,831,644 + CA265_RS23905 0.90 -0.3 5,831,649 - -1.6 5,831,656 + -1.5 5,831,706 + -2.8 5,831,706 + -0.9 5,831,707 - -2.2 5,831,710 + -2.0 5,831,711 - -0.7 5,831,738 + +0.7 5,831,790 + +0.6 5,831,799 + +0.2 5,831,804 - +0.4 5,831,804 - -0.5 5,831,815 - +0.5 5,831,815 - -0.8 5,831,840 + +0.9 5,831,841 - +0.6 5,831,841 - +0.2 5,831,848 - -0.3 5,831,873 - -0.6 5,831,965 - -0.7 5,832,075 - -1.4 5,832,105 - -1.4 5,832,180 - CA265_RS23910 0.12 -0.9 5,832,194 + CA265_RS23910 0.13 +0.3 5,832,195 - CA265_RS23910 0.13 -0.3 5,832,231 + CA265_RS23910 0.15 -0.9 5,832,309 + CA265_RS23910 0.20 -0.5 5,832,349 - CA265_RS23910 0.23 -1.0 5,832,356 - CA265_RS23910 0.23 -0.6 5,832,368 + CA265_RS23910 0.24 +1.6 5,832,382 - CA265_RS23910 0.25 +0.9 5,832,399 + CA265_RS23910 0.26 +1.2 5,832,399 + CA265_RS23910 0.26 +0.2 5,832,437 + CA265_RS23910 0.28 -1.9 5,832,530 - CA265_RS23910 0.34 +0.6 5,832,573 - CA265_RS23910 0.37 -0.5 5,832,592 - CA265_RS23910 0.38 -0.1 5,832,596 - CA265_RS23910 0.38 +0.2 5,832,625 - CA265_RS23910 0.40 -0.0 5,832,636 + CA265_RS23910 0.41 -0.5 5,832,636 + CA265_RS23910 0.41 +2.1 5,832,637 - CA265_RS23910 0.41 +0.1 5,832,637 - CA265_RS23910 0.41 -0.3
Or see this region's nucleotide sequence