Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS21280 and CA265_RS21285 are separated by 35 nucleotides CA265_RS21285 and CA265_RS21290 are separated by 105 nucleotides CA265_RS21290 and CA265_RS21295 overlap by 50 nucleotides
CA265_RS21280: CA265_RS21280 - hypothetical protein, at 5,246,782 to 5,249,715
_RS21280
CA265_RS21285: CA265_RS21285 - DNA-binding response regulator, at 5,249,751 to 5,250,377
_RS21285
CA265_RS21290: CA265_RS21290 - hypothetical protein, at 5,250,483 to 5,250,848
_RS21290
CA265_RS21295: CA265_RS21295 - hypothetical protein, at 5,250,799 to 5,251,719
_RS21295
Position (kb) 5250
5251 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 5249.508 kb on + strand at 5249.509 kb on - strand at 5249.538 kb on - strand at 5249.604 kb on - strand at 5249.633 kb on + strand at 5249.673 kb on + strand at 5249.731 kb on - strand at 5249.731 kb on - strand at 5249.809 kb on - strand at 5249.822 kb on - strand, within CA265_RS21285 at 5249.830 kb on + strand, within CA265_RS21285 at 5249.831 kb on - strand, within CA265_RS21285 at 5249.831 kb on - strand, within CA265_RS21285 at 5249.848 kb on + strand, within CA265_RS21285 at 5249.848 kb on + strand, within CA265_RS21285 at 5249.856 kb on + strand, within CA265_RS21285 at 5249.857 kb on - strand, within CA265_RS21285 at 5249.977 kb on + strand, within CA265_RS21285 at 5249.982 kb on - strand, within CA265_RS21285 at 5249.991 kb on - strand, within CA265_RS21285 at 5249.996 kb on + strand, within CA265_RS21285 at 5250.103 kb on + strand, within CA265_RS21285 at 5250.146 kb on - strand, within CA265_RS21285 at 5250.193 kb on + strand, within CA265_RS21285 at 5250.194 kb on - strand, within CA265_RS21285 at 5250.198 kb on - strand, within CA265_RS21285 at 5250.217 kb on + strand, within CA265_RS21285 at 5250.218 kb on - strand, within CA265_RS21285 at 5250.306 kb on - strand, within CA265_RS21285 at 5250.380 kb on + strand at 5250.380 kb on + strand at 5250.380 kb on + strand at 5250.381 kb on - strand at 5250.466 kb on + strand at 5250.466 kb on + strand at 5250.466 kb on + strand at 5250.508 kb on - strand at 5250.508 kb on - strand at 5250.546 kb on + strand, within CA265_RS21290 at 5250.569 kb on + strand, within CA265_RS21290 at 5250.636 kb on - strand, within CA265_RS21290 at 5250.703 kb on + strand, within CA265_RS21290 at 5250.704 kb on - strand, within CA265_RS21290 at 5250.712 kb on - strand, within CA265_RS21290 at 5250.749 kb on + strand, within CA265_RS21290 at 5250.763 kb on + strand, within CA265_RS21290 at 5250.775 kb on + strand, within CA265_RS21290 at 5250.776 kb on - strand, within CA265_RS21290 at 5250.817 kb on + strand at 5250.830 kb on + strand at 5250.831 kb on - strand at 5250.941 kb on - strand, within CA265_RS21295 at 5250.942 kb on + strand, within CA265_RS21295 at 5250.985 kb on - strand, within CA265_RS21295 at 5251.231 kb on - strand, within CA265_RS21295 at 5251.231 kb on - strand, within CA265_RS21295 at 5251.441 kb on + strand, within CA265_RS21295 at 5251.566 kb on - strand, within CA265_RS21295 at 5251.566 kb on - strand, within CA265_RS21295 at 5251.600 kb on + strand, within CA265_RS21295 at 5251.625 kb on + strand, within CA265_RS21295 at 5251.662 kb on - strand at 5251.696 kb on + strand at 5251.698 kb on + strand at 5251.724 kb on + strand at 5251.725 kb on - strand at 5251.725 kb on - strand at 5251.754 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 5,249,508 + +0.7 5,249,509 - -2.6 5,249,538 - -0.8 5,249,604 - -0.4 5,249,633 + +1.6 5,249,673 + +0.2 5,249,731 - -0.7 5,249,731 - -0.7 5,249,809 - +0.6 5,249,822 - CA265_RS21285 0.11 -0.2 5,249,830 + CA265_RS21285 0.13 -0.6 5,249,831 - CA265_RS21285 0.13 +0.3 5,249,831 - CA265_RS21285 0.13 +1.2 5,249,848 + CA265_RS21285 0.15 -0.2 5,249,848 + CA265_RS21285 0.15 -2.5 5,249,856 + CA265_RS21285 0.17 -1.5 5,249,857 - CA265_RS21285 0.17 -0.9 5,249,977 + CA265_RS21285 0.36 +1.0 5,249,982 - CA265_RS21285 0.37 +0.5 5,249,991 - CA265_RS21285 0.38 +0.0 5,249,996 + CA265_RS21285 0.39 +1.6 5,250,103 + CA265_RS21285 0.56 +1.3 5,250,146 - CA265_RS21285 0.63 +0.3 5,250,193 + CA265_RS21285 0.70 +2.7 5,250,194 - CA265_RS21285 0.71 +0.1 5,250,198 - CA265_RS21285 0.71 -1.1 5,250,217 + CA265_RS21285 0.74 +4.7 5,250,218 - CA265_RS21285 0.74 +0.0 5,250,306 - CA265_RS21285 0.89 +0.2 5,250,380 + +5.5 5,250,380 + +5.5 5,250,380 + +6.1 5,250,381 - -0.0 5,250,466 + +6.5 5,250,466 + +6.1 5,250,466 + +6.7 5,250,508 - -0.1 5,250,508 - -2.9 5,250,546 + CA265_RS21290 0.17 +6.1 5,250,569 + CA265_RS21290 0.23 +6.1 5,250,636 - CA265_RS21290 0.42 +0.3 5,250,703 + CA265_RS21290 0.60 +6.2 5,250,704 - CA265_RS21290 0.60 -0.1 5,250,712 - CA265_RS21290 0.63 +0.2 5,250,749 + CA265_RS21290 0.73 +6.2 5,250,763 + CA265_RS21290 0.77 +6.1 5,250,775 + CA265_RS21290 0.80 +6.0 5,250,776 - CA265_RS21290 0.80 +2.8 5,250,817 + +6.3 5,250,830 + +6.1 5,250,831 - +2.0 5,250,941 - CA265_RS21295 0.15 +0.6 5,250,942 + CA265_RS21295 0.16 +5.8 5,250,985 - CA265_RS21295 0.20 +1.2 5,251,231 - CA265_RS21295 0.47 +0.2 5,251,231 - CA265_RS21295 0.47 -0.0 5,251,441 + CA265_RS21295 0.70 +6.0 5,251,566 - CA265_RS21295 0.83 +0.2 5,251,566 - CA265_RS21295 0.83 -0.1 5,251,600 + CA265_RS21295 0.87 +6.0 5,251,625 + CA265_RS21295 0.90 +5.2 5,251,662 - -1.1 5,251,696 + +4.5 5,251,698 + +5.0 5,251,724 + +3.2 5,251,725 - -0.5 5,251,725 - +1.0 5,251,754 + +2.8
Or see this region's nucleotide sequence