Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS20740 and CA265_RS20745 are separated by 3 nucleotides CA265_RS20745 and CA265_RS20750 are separated by 163 nucleotides CA265_RS20750 and CA265_RS20755 are separated by 2 nucleotides
CA265_RS20740: CA265_RS20740 - plasmid transfer protein, at 5,124,912 to 5,126,054
_RS20740
CA265_RS20745: CA265_RS20745 - conjugative transposon protein TraK, at 5,126,058 to 5,126,675
_RS20745
CA265_RS20750: CA265_RS20750 - hypothetical protein, at 5,126,839 to 5,127,066
_RS20750
CA265_RS20755: CA265_RS20755 - hypothetical protein, at 5,127,069 to 5,128,277
_RS20755
Position (kb) 5126
5127 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5125.069 kb on + strand, within CA265_RS20740 at 5125.070 kb on - strand, within CA265_RS20740 at 5125.229 kb on - strand, within CA265_RS20740 at 5125.274 kb on - strand, within CA265_RS20740 at 5125.591 kb on - strand, within CA265_RS20740 at 5125.594 kb on + strand, within CA265_RS20740 at 5125.597 kb on + strand, within CA265_RS20740 at 5125.598 kb on - strand, within CA265_RS20740 at 5125.601 kb on + strand, within CA265_RS20740 at 5125.639 kb on + strand, within CA265_RS20740 at 5125.639 kb on + strand, within CA265_RS20740 at 5125.665 kb on - strand, within CA265_RS20740 at 5125.695 kb on - strand, within CA265_RS20740 at 5125.695 kb on - strand, within CA265_RS20740 at 5125.743 kb on + strand, within CA265_RS20740 at 5125.755 kb on + strand, within CA265_RS20740 at 5125.756 kb on - strand, within CA265_RS20740 at 5125.756 kb on - strand, within CA265_RS20740 at 5125.780 kb on - strand, within CA265_RS20740 at 5125.866 kb on - strand, within CA265_RS20740 at 5125.873 kb on + strand, within CA265_RS20740 at 5126.052 kb on + strand at 5126.052 kb on + strand at 5126.052 kb on + strand at 5126.052 kb on + strand at 5126.170 kb on + strand, within CA265_RS20745 at 5126.193 kb on + strand, within CA265_RS20745 at 5126.194 kb on - strand, within CA265_RS20745 at 5126.265 kb on + strand, within CA265_RS20745 at 5126.309 kb on + strand, within CA265_RS20745 at 5126.500 kb on - strand, within CA265_RS20745 at 5126.516 kb on - strand, within CA265_RS20745 at 5126.783 kb on + strand at 5126.783 kb on + strand at 5126.786 kb on - strand at 5126.786 kb on - strand at 5126.833 kb on - strand at 5126.833 kb on - strand at 5126.873 kb on + strand, within CA265_RS20750 at 5126.916 kb on - strand, within CA265_RS20750 at 5127.029 kb on + strand, within CA265_RS20750 at 5127.030 kb on - strand, within CA265_RS20750 at 5127.076 kb on + strand at 5127.096 kb on + strand at 5127.096 kb on + strand at 5127.097 kb on - strand at 5127.097 kb on - strand at 5127.097 kb on - strand at 5127.100 kb on + strand at 5127.113 kb on - strand at 5127.125 kb on + strand at 5127.126 kb on - strand at 5127.147 kb on + strand at 5127.149 kb on + strand at 5127.149 kb on + strand at 5127.247 kb on + strand, within CA265_RS20755 at 5127.358 kb on - strand, within CA265_RS20755 at 5127.377 kb on + strand, within CA265_RS20755 at 5127.378 kb on - strand, within CA265_RS20755 at 5127.599 kb on - strand, within CA265_RS20755 at 5127.623 kb on - strand, within CA265_RS20755
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 5,125,069 + CA265_RS20740 0.14 -1.0 5,125,070 - CA265_RS20740 0.14 +0.0 5,125,229 - CA265_RS20740 0.28 -0.6 5,125,274 - CA265_RS20740 0.32 -0.8 5,125,591 - CA265_RS20740 0.59 -1.0 5,125,594 + CA265_RS20740 0.60 +0.4 5,125,597 + CA265_RS20740 0.60 +0.7 5,125,598 - CA265_RS20740 0.60 +0.5 5,125,601 + CA265_RS20740 0.60 -0.5 5,125,639 + CA265_RS20740 0.64 +1.1 5,125,639 + CA265_RS20740 0.64 -0.7 5,125,665 - CA265_RS20740 0.66 -1.1 5,125,695 - CA265_RS20740 0.69 -0.6 5,125,695 - CA265_RS20740 0.69 -0.9 5,125,743 + CA265_RS20740 0.73 +0.6 5,125,755 + CA265_RS20740 0.74 +1.2 5,125,756 - CA265_RS20740 0.74 -3.0 5,125,756 - CA265_RS20740 0.74 +0.7 5,125,780 - CA265_RS20740 0.76 +0.4 5,125,866 - CA265_RS20740 0.83 -1.2 5,125,873 + CA265_RS20740 0.84 +1.1 5,126,052 + +0.6 5,126,052 + +2.4 5,126,052 + +1.1 5,126,052 + +0.7 5,126,170 + CA265_RS20745 0.18 +1.6 5,126,193 + CA265_RS20745 0.22 +2.5 5,126,194 - CA265_RS20745 0.22 -0.1 5,126,265 + CA265_RS20745 0.33 +2.3 5,126,309 + CA265_RS20745 0.41 +2.6 5,126,500 - CA265_RS20745 0.72 +1.2 5,126,516 - CA265_RS20745 0.74 +0.4 5,126,783 + +1.6 5,126,783 + +1.9 5,126,786 - -0.3 5,126,786 - -0.2 5,126,833 - -0.3 5,126,833 - -0.5 5,126,873 + CA265_RS20750 0.15 +1.5 5,126,916 - CA265_RS20750 0.34 +0.6 5,127,029 + CA265_RS20750 0.83 +1.1 5,127,030 - CA265_RS20750 0.84 -0.2 5,127,076 + +1.5 5,127,096 + +1.1 5,127,096 + +0.9 5,127,097 - -0.9 5,127,097 - -0.4 5,127,097 - +0.3 5,127,100 + +0.7 5,127,113 - -0.7 5,127,125 + +2.2 5,127,126 - +1.3 5,127,147 + +0.6 5,127,149 + -0.2 5,127,149 + +1.1 5,127,247 + CA265_RS20755 0.15 +1.7 5,127,358 - CA265_RS20755 0.24 -0.9 5,127,377 + CA265_RS20755 0.25 +1.4 5,127,378 - CA265_RS20755 0.26 -1.0 5,127,599 - CA265_RS20755 0.44 -1.1 5,127,623 - CA265_RS20755 0.46 -2.1
Or see this region's nucleotide sequence