Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS20000 and CA265_RS20005 are separated by 167 nucleotides CA265_RS20005 and CA265_RS20010 are separated by 3 nucleotides CA265_RS20010 and CA265_RS20015 are separated by 116 nucleotides
CA265_RS20000: CA265_RS20000 - sigma-54-dependent Fis family transcriptional regulator, at 4,945,313 to 4,946,722
_RS20000
CA265_RS20005: CA265_RS20005 - enoyl-CoA hydratase, at 4,946,890 to 4,947,669
_RS20005
CA265_RS20010: CA265_RS20010 - methylmalonyl-CoA mutase, at 4,947,673 to 4,948,092
_RS20010
CA265_RS20015: CA265_RS20015 - glutamine--tRNA ligase, at 4,948,209 to 4,949,864
_RS20015
Position (kb)
4946
4947
4948 Strain fitness (log2 ratio)
-2
-1
0
1 at 4945.906 kb on + strand, within CA265_RS20000 at 4945.907 kb on - strand, within CA265_RS20000 at 4945.922 kb on + strand, within CA265_RS20000 at 4945.954 kb on + strand, within CA265_RS20000 at 4945.998 kb on - strand, within CA265_RS20000 at 4946.213 kb on - strand, within CA265_RS20000 at 4946.220 kb on + strand, within CA265_RS20000 at 4946.279 kb on - strand, within CA265_RS20000 at 4946.288 kb on - strand, within CA265_RS20000 at 4946.341 kb on + strand, within CA265_RS20000 at 4946.386 kb on + strand, within CA265_RS20000 at 4946.386 kb on + strand, within CA265_RS20000 at 4946.390 kb on + strand, within CA265_RS20000 at 4946.416 kb on + strand, within CA265_RS20000 at 4946.424 kb on + strand, within CA265_RS20000 at 4946.431 kb on - strand, within CA265_RS20000 at 4946.465 kb on - strand, within CA265_RS20000 at 4946.489 kb on - strand, within CA265_RS20000 at 4946.489 kb on - strand, within CA265_RS20000 at 4946.529 kb on - strand, within CA265_RS20000 at 4946.535 kb on + strand, within CA265_RS20000 at 4946.607 kb on - strand at 4946.615 kb on - strand at 4946.629 kb on + strand at 4946.629 kb on + strand at 4946.630 kb on - strand at 4946.630 kb on - strand at 4946.634 kb on - strand at 4946.634 kb on - strand at 4946.704 kb on + strand at 4946.704 kb on + strand at 4946.708 kb on + strand at 4946.709 kb on - strand at 4946.741 kb on + strand at 4946.800 kb on - strand at 4946.811 kb on - strand at 4946.891 kb on + strand at 4946.992 kb on - strand, within CA265_RS20005 at 4947.009 kb on + strand, within CA265_RS20005 at 4947.009 kb on + strand, within CA265_RS20005 at 4947.130 kb on + strand, within CA265_RS20005 at 4947.168 kb on + strand at 4947.191 kb on - strand, within CA265_RS20005 at 4947.199 kb on - strand, within CA265_RS20005 at 4947.261 kb on + strand, within CA265_RS20005 at 4947.261 kb on + strand, within CA265_RS20005 at 4947.261 kb on + strand, within CA265_RS20005 at 4947.304 kb on + strand, within CA265_RS20005 at 4947.305 kb on - strand, within CA265_RS20005 at 4947.305 kb on - strand, within CA265_RS20005 at 4947.366 kb on + strand, within CA265_RS20005 at 4947.397 kb on - strand, within CA265_RS20005 at 4947.448 kb on - strand, within CA265_RS20005 at 4947.448 kb on - strand, within CA265_RS20005 at 4947.449 kb on + strand, within CA265_RS20005 at 4947.465 kb on + strand, within CA265_RS20005 at 4947.514 kb on - strand, within CA265_RS20005 at 4947.524 kb on - strand, within CA265_RS20005 at 4947.524 kb on - strand, within CA265_RS20005 at 4947.537 kb on + strand, within CA265_RS20005 at 4947.583 kb on - strand, within CA265_RS20005 at 4947.625 kb on + strand at 4947.669 kb on + strand at 4947.670 kb on - strand at 4947.708 kb on + strand at 4947.708 kb on + strand at 4947.710 kb on + strand at 4947.710 kb on + strand at 4947.711 kb on - strand at 4947.711 kb on - strand at 4947.711 kb on - strand at 4947.711 kb on - strand at 4947.711 kb on - strand at 4947.720 kb on + strand, within CA265_RS20010 at 4947.721 kb on - strand, within CA265_RS20010 at 4947.748 kb on + strand, within CA265_RS20010 at 4947.799 kb on - strand, within CA265_RS20010 at 4947.830 kb on - strand, within CA265_RS20010 at 4947.895 kb on - strand, within CA265_RS20010 at 4947.976 kb on + strand, within CA265_RS20010 at 4948.039 kb on + strand, within CA265_RS20010 at 4948.040 kb on - strand, within CA265_RS20010 at 4948.069 kb on - strand at 4948.147 kb on - strand at 4948.170 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 4,945,906 + CA265_RS20000 0.42 +1.3 4,945,907 - CA265_RS20000 0.42 +0.3 4,945,922 + CA265_RS20000 0.43 -1.6 4,945,954 + CA265_RS20000 0.45 -0.4 4,945,998 - CA265_RS20000 0.49 -1.6 4,946,213 - CA265_RS20000 0.64 -1.1 4,946,220 + CA265_RS20000 0.64 -0.2 4,946,279 - CA265_RS20000 0.69 -1.2 4,946,288 - CA265_RS20000 0.69 -2.2 4,946,341 + CA265_RS20000 0.73 -0.9 4,946,386 + CA265_RS20000 0.76 -0.4 4,946,386 + CA265_RS20000 0.76 +1.1 4,946,390 + CA265_RS20000 0.76 -0.8 4,946,416 + CA265_RS20000 0.78 -0.2 4,946,424 + CA265_RS20000 0.79 -0.5 4,946,431 - CA265_RS20000 0.79 -0.0 4,946,465 - CA265_RS20000 0.82 -0.6 4,946,489 - CA265_RS20000 0.83 +0.1 4,946,489 - CA265_RS20000 0.83 -0.0 4,946,529 - CA265_RS20000 0.86 -1.5 4,946,535 + CA265_RS20000 0.87 +0.1 4,946,607 - -0.7 4,946,615 - -0.5 4,946,629 + -0.4 4,946,629 + -0.1 4,946,630 - -1.2 4,946,630 - -1.7 4,946,634 - -0.9 4,946,634 - -1.5 4,946,704 + -0.8 4,946,704 + +0.4 4,946,708 + -1.0 4,946,709 - -0.4 4,946,741 + -1.8 4,946,800 - -0.3 4,946,811 - -0.6 4,946,891 + +0.6 4,946,992 - CA265_RS20005 0.13 -1.5 4,947,009 + CA265_RS20005 0.15 +1.1 4,947,009 + CA265_RS20005 0.15 +0.2 4,947,130 + CA265_RS20005 0.31 -0.8 4,947,168 + +0.1 4,947,191 - CA265_RS20005 0.39 -0.0 4,947,199 - CA265_RS20005 0.40 -2.3 4,947,261 + CA265_RS20005 0.48 -1.1 4,947,261 + CA265_RS20005 0.48 -0.2 4,947,261 + CA265_RS20005 0.48 -0.1 4,947,304 + CA265_RS20005 0.53 -0.4 4,947,305 - CA265_RS20005 0.53 -0.7 4,947,305 - CA265_RS20005 0.53 -1.4 4,947,366 + CA265_RS20005 0.61 -0.1 4,947,397 - CA265_RS20005 0.65 -0.4 4,947,448 - CA265_RS20005 0.72 -0.9 4,947,448 - CA265_RS20005 0.72 -1.1 4,947,449 + CA265_RS20005 0.72 -1.0 4,947,465 + CA265_RS20005 0.74 -1.8 4,947,514 - CA265_RS20005 0.80 -0.0 4,947,524 - CA265_RS20005 0.81 -1.0 4,947,524 - CA265_RS20005 0.81 -2.1 4,947,537 + CA265_RS20005 0.83 -1.0 4,947,583 - CA265_RS20005 0.89 -0.7 4,947,625 + -0.1 4,947,669 + +0.3 4,947,670 - -2.2 4,947,708 + +0.0 4,947,708 + +0.0 4,947,710 + +0.5 4,947,710 + +0.3 4,947,711 - -0.8 4,947,711 - -0.9 4,947,711 - -1.0 4,947,711 - -0.6 4,947,711 - -1.9 4,947,720 + CA265_RS20010 0.11 +0.3 4,947,721 - CA265_RS20010 0.11 -0.5 4,947,748 + CA265_RS20010 0.18 -0.7 4,947,799 - CA265_RS20010 0.30 -0.5 4,947,830 - CA265_RS20010 0.37 +1.2 4,947,895 - CA265_RS20010 0.53 -1.0 4,947,976 + CA265_RS20010 0.72 -0.1 4,948,039 + CA265_RS20010 0.87 -0.4 4,948,040 - CA265_RS20010 0.87 -0.0 4,948,069 - +0.3 4,948,147 - -0.1 4,948,170 + -0.1
Or see this region's nucleotide sequence