Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS18530 and CA265_RS18535 are separated by 33 nucleotides CA265_RS18535 and CA265_RS18540 are separated by 40 nucleotides CA265_RS18540 and CA265_RS18545 are separated by 89 nucleotides
CA265_RS18530: CA265_RS18530 - aspartate aminotransferase family protein, at 4,606,649 to 4,607,797
_RS18530
CA265_RS18535: CA265_RS18535 - four helix bundle protein, at 4,607,831 to 4,608,202
_RS18535
CA265_RS18540: CA265_RS18540 - N-succinylglutamylphosphate reductase (EC 1.2.1.38) (from data) , at 4,608,243 to 4,609,223
_RS18540
CA265_RS18545: CA265_RS18545 - argininosuccinate synthase, at 4,609,313 to 4,610,500
_RS18545
Position (kb)
4608
4609
4610 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4607.586 kb on - strand, within CA265_RS18530 at 4607.627 kb on + strand, within CA265_RS18530 at 4607.809 kb on + strand at 4607.809 kb on + strand at 4607.810 kb on - strand at 4607.811 kb on + strand at 4607.877 kb on - strand, within CA265_RS18535 at 4607.910 kb on - strand, within CA265_RS18535 at 4607.957 kb on + strand, within CA265_RS18535 at 4607.972 kb on + strand, within CA265_RS18535 at 4608.096 kb on - strand, within CA265_RS18535 at 4608.151 kb on + strand, within CA265_RS18535 at 4608.152 kb on - strand, within CA265_RS18535 at 4608.156 kb on - strand, within CA265_RS18535 at 4608.207 kb on + strand at 4608.240 kb on + strand at 4608.470 kb on + strand, within CA265_RS18540 at 4608.505 kb on - strand, within CA265_RS18540 at 4608.671 kb on + strand, within CA265_RS18540 at 4608.734 kb on + strand, within CA265_RS18540 at 4608.804 kb on - strand, within CA265_RS18540 at 4608.957 kb on - strand, within CA265_RS18540 at 4609.028 kb on + strand, within CA265_RS18540 at 4609.082 kb on + strand, within CA265_RS18540 at 4609.083 kb on - strand, within CA265_RS18540 at 4609.086 kb on + strand, within CA265_RS18540 at 4609.099 kb on - strand, within CA265_RS18540 at 4609.293 kb on + strand at 4609.293 kb on + strand at 4609.293 kb on + strand at 4609.328 kb on + strand at 4609.362 kb on - strand at 4609.416 kb on + strand at 4609.430 kb on - strand at 4609.450 kb on + strand, within CA265_RS18545 at 4609.451 kb on - strand, within CA265_RS18545 at 4609.451 kb on - strand, within CA265_RS18545 at 4609.451 kb on - strand, within CA265_RS18545 at 4609.494 kb on - strand, within CA265_RS18545 at 4609.608 kb on - strand, within CA265_RS18545 at 4609.672 kb on + strand, within CA265_RS18545 at 4609.857 kb on - strand, within CA265_RS18545 at 4609.857 kb on - strand, within CA265_RS18545 at 4609.862 kb on + strand, within CA265_RS18545 at 4610.005 kb on - strand, within CA265_RS18545 at 4610.005 kb on - strand, within CA265_RS18545 at 4610.066 kb on - strand, within CA265_RS18545 at 4610.095 kb on + strand, within CA265_RS18545 at 4610.096 kb on - strand, within CA265_RS18545 at 4610.104 kb on + strand, within CA265_RS18545 at 4610.113 kb on + strand, within CA265_RS18545 at 4610.187 kb on + strand, within CA265_RS18545 at 4610.188 kb on - strand, within CA265_RS18545
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 4,607,586 - CA265_RS18530 0.82 -1.0 4,607,627 + CA265_RS18530 0.85 -2.0 4,607,809 + -0.6 4,607,809 + -0.7 4,607,810 - -0.9 4,607,811 + -1.0 4,607,877 - CA265_RS18535 0.12 -0.4 4,607,910 - CA265_RS18535 0.21 +0.1 4,607,957 + CA265_RS18535 0.34 -0.1 4,607,972 + CA265_RS18535 0.38 -0.3 4,608,096 - CA265_RS18535 0.71 -0.8 4,608,151 + CA265_RS18535 0.86 -1.5 4,608,152 - CA265_RS18535 0.86 -1.0 4,608,156 - CA265_RS18535 0.87 -1.3 4,608,207 + -1.0 4,608,240 + -0.6 4,608,470 + CA265_RS18540 0.23 -0.9 4,608,505 - CA265_RS18540 0.27 -0.9 4,608,671 + CA265_RS18540 0.44 -0.0 4,608,734 + CA265_RS18540 0.50 -0.9 4,608,804 - CA265_RS18540 0.57 -1.0 4,608,957 - CA265_RS18540 0.73 -1.7 4,609,028 + CA265_RS18540 0.80 -1.5 4,609,082 + CA265_RS18540 0.86 -0.2 4,609,083 - CA265_RS18540 0.86 -1.8 4,609,086 + CA265_RS18540 0.86 -0.9 4,609,099 - CA265_RS18540 0.87 -0.9 4,609,293 + -0.0 4,609,293 + -0.0 4,609,293 + -1.4 4,609,328 + -1.6 4,609,362 - -0.9 4,609,416 + +0.4 4,609,430 - -0.9 4,609,450 + CA265_RS18545 0.12 -1.0 4,609,451 - CA265_RS18545 0.12 -0.4 4,609,451 - CA265_RS18545 0.12 +1.0 4,609,451 - CA265_RS18545 0.12 -0.9 4,609,494 - CA265_RS18545 0.15 +0.2 4,609,608 - CA265_RS18545 0.25 +0.1 4,609,672 + CA265_RS18545 0.30 -1.0 4,609,857 - CA265_RS18545 0.46 +1.1 4,609,857 - CA265_RS18545 0.46 +0.4 4,609,862 + CA265_RS18545 0.46 +0.5 4,610,005 - CA265_RS18545 0.58 -0.0 4,610,005 - CA265_RS18545 0.58 -0.0 4,610,066 - CA265_RS18545 0.63 -0.2 4,610,095 + CA265_RS18545 0.66 -0.2 4,610,096 - CA265_RS18545 0.66 -0.9 4,610,104 + CA265_RS18545 0.67 +0.5 4,610,113 + CA265_RS18545 0.67 -2.9 4,610,187 + CA265_RS18545 0.74 -3.0 4,610,188 - CA265_RS18545 0.74 -0.1
Or see this region's nucleotide sequence