Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS18220 and CA265_RS18225 are separated by 159 nucleotides CA265_RS18225 and CA265_RS18230 are separated by 78 nucleotides CA265_RS18230 and CA265_RS18235 are separated by 3 nucleotides
CA265_RS18220: CA265_RS18220 - metallophosphatase, at 4,543,002 to 4,544,258
_RS18220
CA265_RS18225: CA265_RS18225 - DNA-binding response regulator, at 4,544,418 to 4,545,107
_RS18225
CA265_RS18230: CA265_RS18230 - two-component sensor histidine kinase, at 4,545,186 to 4,546,859
_RS18230
CA265_RS18235: CA265_RS18235 - peptidase, at 4,546,863 to 4,548,746
_RS18235
Position (kb)
4545
4546
4547 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4544.195 kb on - strand at 4544.237 kb on - strand at 4544.257 kb on - strand at 4544.257 kb on - strand at 4544.257 kb on - strand at 4544.257 kb on - strand at 4544.257 kb on - strand at 4544.366 kb on + strand at 4544.371 kb on + strand at 4544.372 kb on - strand at 4544.379 kb on + strand at 4544.379 kb on + strand at 4544.380 kb on - strand at 4544.380 kb on - strand at 4544.411 kb on - strand at 4544.413 kb on + strand at 4544.413 kb on + strand at 4544.414 kb on - strand at 4544.415 kb on + strand at 4544.442 kb on + strand at 4544.443 kb on - strand at 4544.490 kb on + strand, within CA265_RS18225 at 4544.513 kb on + strand, within CA265_RS18225 at 4544.513 kb on + strand, within CA265_RS18225 at 4544.535 kb on - strand, within CA265_RS18225 at 4544.538 kb on + strand, within CA265_RS18225 at 4544.571 kb on + strand, within CA265_RS18225 at 4544.643 kb on + strand, within CA265_RS18225 at 4544.699 kb on + strand, within CA265_RS18225 at 4544.699 kb on + strand, within CA265_RS18225 at 4544.699 kb on + strand, within CA265_RS18225 at 4544.700 kb on - strand, within CA265_RS18225 at 4544.703 kb on - strand, within CA265_RS18225 at 4544.802 kb on - strand, within CA265_RS18225 at 4544.804 kb on - strand, within CA265_RS18225 at 4544.824 kb on + strand, within CA265_RS18225 at 4544.868 kb on - strand, within CA265_RS18225 at 4544.871 kb on - strand, within CA265_RS18225 at 4544.946 kb on + strand, within CA265_RS18225 at 4544.957 kb on + strand, within CA265_RS18225 at 4544.957 kb on + strand, within CA265_RS18225 at 4545.021 kb on - strand, within CA265_RS18225 at 4545.021 kb on - strand, within CA265_RS18225 at 4545.037 kb on - strand, within CA265_RS18225 at 4545.078 kb on + strand at 4545.112 kb on - strand at 4545.120 kb on + strand at 4545.173 kb on + strand at 4545.173 kb on + strand at 4545.194 kb on + strand at 4545.194 kb on + strand at 4545.196 kb on + strand at 4545.196 kb on + strand at 4545.196 kb on + strand at 4545.231 kb on + strand at 4545.323 kb on - strand at 4545.354 kb on + strand, within CA265_RS18230 at 4545.475 kb on + strand, within CA265_RS18230 at 4545.502 kb on - strand, within CA265_RS18230 at 4545.530 kb on + strand, within CA265_RS18230 at 4545.540 kb on - strand, within CA265_RS18230 at 4545.556 kb on + strand, within CA265_RS18230 at 4545.557 kb on - strand, within CA265_RS18230 at 4545.623 kb on + strand, within CA265_RS18230 at 4545.624 kb on - strand, within CA265_RS18230 at 4545.624 kb on - strand, within CA265_RS18230 at 4545.648 kb on - strand, within CA265_RS18230 at 4545.671 kb on + strand, within CA265_RS18230 at 4545.693 kb on + strand, within CA265_RS18230 at 4545.693 kb on + strand, within CA265_RS18230 at 4545.726 kb on + strand, within CA265_RS18230 at 4545.726 kb on + strand, within CA265_RS18230 at 4545.768 kb on - strand, within CA265_RS18230 at 4545.872 kb on + strand, within CA265_RS18230 at 4545.926 kb on - strand, within CA265_RS18230 at 4545.928 kb on - strand, within CA265_RS18230 at 4546.013 kb on + strand, within CA265_RS18230 at 4546.014 kb on - strand, within CA265_RS18230 at 4546.089 kb on + strand, within CA265_RS18230 at 4546.131 kb on - strand, within CA265_RS18230 at 4546.144 kb on - strand, within CA265_RS18230 at 4546.220 kb on + strand, within CA265_RS18230 at 4546.229 kb on - strand, within CA265_RS18230 at 4546.236 kb on + strand, within CA265_RS18230 at 4546.237 kb on - strand, within CA265_RS18230 at 4546.256 kb on + strand, within CA265_RS18230 at 4546.330 kb on - strand, within CA265_RS18230 at 4546.430 kb on - strand, within CA265_RS18230 at 4546.582 kb on - strand, within CA265_RS18230 at 4546.614 kb on - strand, within CA265_RS18230 at 4546.623 kb on + strand, within CA265_RS18230 at 4546.636 kb on + strand, within CA265_RS18230 at 4546.639 kb on + strand, within CA265_RS18230 at 4546.678 kb on + strand, within CA265_RS18230 at 4546.679 kb on - strand, within CA265_RS18230 at 4546.688 kb on + strand, within CA265_RS18230 at 4546.719 kb on + strand at 4546.720 kb on - strand at 4546.749 kb on - strand at 4546.751 kb on - strand at 4546.779 kb on - strand at 4546.909 kb on + strand at 4546.969 kb on + strand at 4546.974 kb on - strand at 4547.030 kb on + strand at 4547.114 kb on + strand, within CA265_RS18235 at 4547.155 kb on + strand, within CA265_RS18235 at 4547.155 kb on + strand, within CA265_RS18235 at 4547.155 kb on + strand, within CA265_RS18235 at 4547.156 kb on - strand, within CA265_RS18235 at 4547.205 kb on - strand, within CA265_RS18235 at 4547.217 kb on + strand, within CA265_RS18235 at 4547.282 kb on + strand, within CA265_RS18235 at 4547.285 kb on - strand, within CA265_RS18235 at 4547.356 kb on - strand, within CA265_RS18235 at 4547.413 kb on + strand, within CA265_RS18235 at 4547.413 kb on + strand, within CA265_RS18235 at 4547.428 kb on + strand, within CA265_RS18235 at 4547.435 kb on + strand, within CA265_RS18235 at 4547.446 kb on + strand, within CA265_RS18235 at 4547.502 kb on - strand, within CA265_RS18235 at 4547.502 kb on - strand, within CA265_RS18235 at 4547.504 kb on - strand, within CA265_RS18235 at 4547.534 kb on + strand, within CA265_RS18235 at 4547.535 kb on - strand, within CA265_RS18235 at 4547.535 kb on - strand, within CA265_RS18235 at 4547.557 kb on - strand, within CA265_RS18235 at 4547.593 kb on + strand, within CA265_RS18235 at 4547.678 kb on + strand, within CA265_RS18235 at 4547.679 kb on - strand, within CA265_RS18235 at 4547.714 kb on + strand, within CA265_RS18235 at 4547.714 kb on + strand, within CA265_RS18235 at 4547.718 kb on + strand, within CA265_RS18235 at 4547.718 kb on + strand, within CA265_RS18235 at 4547.723 kb on - strand, within CA265_RS18235 at 4547.723 kb on - strand, within CA265_RS18235 at 4547.837 kb on - strand, within CA265_RS18235 at 4547.850 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 4,544,195 - -0.7 4,544,237 - -1.4 4,544,257 - -1.2 4,544,257 - -1.1 4,544,257 - +0.3 4,544,257 - -1.0 4,544,257 - +0.8 4,544,366 + +0.0 4,544,371 + +0.7 4,544,372 - -1.1 4,544,379 + +0.6 4,544,379 + +0.5 4,544,380 - +0.4 4,544,380 - -0.3 4,544,411 - -0.9 4,544,413 + +0.5 4,544,413 + -0.1 4,544,414 - -1.1 4,544,415 + +0.8 4,544,442 + +2.4 4,544,443 - +1.5 4,544,490 + CA265_RS18225 0.10 +3.2 4,544,513 + CA265_RS18225 0.14 +2.6 4,544,513 + CA265_RS18225 0.14 +2.5 4,544,535 - CA265_RS18225 0.17 +3.0 4,544,538 + CA265_RS18225 0.17 +3.4 4,544,571 + CA265_RS18225 0.22 +2.4 4,544,643 + CA265_RS18225 0.33 +2.8 4,544,699 + CA265_RS18225 0.41 +2.8 4,544,699 + CA265_RS18225 0.41 +2.9 4,544,699 + CA265_RS18225 0.41 +2.2 4,544,700 - CA265_RS18225 0.41 +2.3 4,544,703 - CA265_RS18225 0.41 +2.7 4,544,802 - CA265_RS18225 0.56 +3.5 4,544,804 - CA265_RS18225 0.56 +3.5 4,544,824 + CA265_RS18225 0.59 +3.1 4,544,868 - CA265_RS18225 0.65 +1.9 4,544,871 - CA265_RS18225 0.66 +2.6 4,544,946 + CA265_RS18225 0.77 +2.7 4,544,957 + CA265_RS18225 0.78 +2.9 4,544,957 + CA265_RS18225 0.78 +2.5 4,545,021 - CA265_RS18225 0.87 +1.7 4,545,021 - CA265_RS18225 0.87 +3.3 4,545,037 - CA265_RS18225 0.90 +3.5 4,545,078 + +3.2 4,545,112 - +0.5 4,545,120 + +2.9 4,545,173 + +2.5 4,545,173 + +1.8 4,545,194 + +3.2 4,545,194 + +2.3 4,545,196 + +2.8 4,545,196 + +2.8 4,545,196 + +3.1 4,545,231 + +3.5 4,545,323 - -2.4 4,545,354 + CA265_RS18230 0.10 +3.4 4,545,475 + CA265_RS18230 0.17 +1.6 4,545,502 - CA265_RS18230 0.19 -1.9 4,545,530 + CA265_RS18230 0.21 +3.3 4,545,540 - CA265_RS18230 0.21 -1.3 4,545,556 + CA265_RS18230 0.22 +3.4 4,545,557 - CA265_RS18230 0.22 -2.4 4,545,623 + CA265_RS18230 0.26 +3.1 4,545,624 - CA265_RS18230 0.26 -3.1 4,545,624 - CA265_RS18230 0.26 -1.6 4,545,648 - CA265_RS18230 0.28 -1.8 4,545,671 + CA265_RS18230 0.29 +3.4 4,545,693 + CA265_RS18230 0.30 +3.5 4,545,693 + CA265_RS18230 0.30 +2.7 4,545,726 + CA265_RS18230 0.32 +2.1 4,545,726 + CA265_RS18230 0.32 +3.2 4,545,768 - CA265_RS18230 0.35 -0.9 4,545,872 + CA265_RS18230 0.41 +2.4 4,545,926 - CA265_RS18230 0.44 -2.4 4,545,928 - CA265_RS18230 0.44 -3.5 4,546,013 + CA265_RS18230 0.49 +2.8 4,546,014 - CA265_RS18230 0.49 -1.4 4,546,089 + CA265_RS18230 0.54 +3.4 4,546,131 - CA265_RS18230 0.56 -2.4 4,546,144 - CA265_RS18230 0.57 -0.1 4,546,220 + CA265_RS18230 0.62 +3.4 4,546,229 - CA265_RS18230 0.62 -0.1 4,546,236 + CA265_RS18230 0.63 +2.6 4,546,237 - CA265_RS18230 0.63 -2.4 4,546,256 + CA265_RS18230 0.64 +2.4 4,546,330 - CA265_RS18230 0.68 -2.5 4,546,430 - CA265_RS18230 0.74 -3.9 4,546,582 - CA265_RS18230 0.83 -3.3 4,546,614 - CA265_RS18230 0.85 -3.9 4,546,623 + CA265_RS18230 0.86 +3.5 4,546,636 + CA265_RS18230 0.87 +2.1 4,546,639 + CA265_RS18230 0.87 +3.0 4,546,678 + CA265_RS18230 0.89 +2.6 4,546,679 - CA265_RS18230 0.89 -1.8 4,546,688 + CA265_RS18230 0.90 +2.6 4,546,719 + +3.0 4,546,720 - -3.5 4,546,749 - -1.0 4,546,751 - -0.1 4,546,779 - -3.9 4,546,909 + +2.1 4,546,969 + +3.3 4,546,974 - -3.6 4,547,030 + +2.0 4,547,114 + CA265_RS18235 0.13 +2.9 4,547,155 + CA265_RS18235 0.15 +2.9 4,547,155 + CA265_RS18235 0.15 +2.5 4,547,155 + CA265_RS18235 0.15 +2.6 4,547,156 - CA265_RS18235 0.16 -2.8 4,547,205 - CA265_RS18235 0.18 -1.5 4,547,217 + CA265_RS18235 0.19 -2.6 4,547,282 + CA265_RS18235 0.22 -0.1 4,547,285 - CA265_RS18235 0.22 -2.0 4,547,356 - CA265_RS18235 0.26 -2.0 4,547,413 + CA265_RS18235 0.29 -0.8 4,547,413 + CA265_RS18235 0.29 -1.1 4,547,428 + CA265_RS18235 0.30 -0.6 4,547,435 + CA265_RS18235 0.30 -0.2 4,547,446 + CA265_RS18235 0.31 -0.3 4,547,502 - CA265_RS18235 0.34 -2.9 4,547,502 - CA265_RS18235 0.34 -2.2 4,547,504 - CA265_RS18235 0.34 +0.3 4,547,534 + CA265_RS18235 0.36 -0.1 4,547,535 - CA265_RS18235 0.36 -2.5 4,547,535 - CA265_RS18235 0.36 -3.4 4,547,557 - CA265_RS18235 0.37 +0.3 4,547,593 + CA265_RS18235 0.39 -0.0 4,547,678 + CA265_RS18235 0.43 -2.1 4,547,679 - CA265_RS18235 0.43 -3.3 4,547,714 + CA265_RS18235 0.45 -1.6 4,547,714 + CA265_RS18235 0.45 -1.1 4,547,718 + CA265_RS18235 0.45 -1.7 4,547,718 + CA265_RS18235 0.45 -0.7 4,547,723 - CA265_RS18235 0.46 -1.7 4,547,723 - CA265_RS18235 0.46 -3.9 4,547,837 - CA265_RS18235 0.52 -3.7 4,547,850 - +0.3
Or see this region's nucleotide sequence