Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS18180 and CA265_RS18185 are separated by 80 nucleotides CA265_RS18185 and CA265_RS18190 are separated by 128 nucleotides CA265_RS18190 and CA265_RS18195 are separated by 127 nucleotides CA265_RS18195 and CA265_RS18200 are separated by 228 nucleotides
CA265_RS18180: CA265_RS18180 - cupin, at 4,534,936 to 4,535,817
_RS18180
CA265_RS18185: CA265_RS18185 - heme oxygenase, at 4,535,898 to 4,536,683
_RS18185
CA265_RS18190: CA265_RS18190 - cupin, at 4,536,812 to 4,537,690
_RS18190
CA265_RS18195: CA265_RS18195 - maltose O-acetyltransferase, at 4,537,818 to 4,538,420
_RS18195
CA265_RS18200: CA265_RS18200 - DUF4440 domain-containing protein, at 4,538,649 to 4,539,074
_RS18200
Position (kb)
4536
4537
4538 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4535.874 kb on + strand at 4535.875 kb on - strand at 4536.050 kb on - strand, within CA265_RS18185 at 4536.062 kb on - strand, within CA265_RS18185 at 4536.083 kb on + strand, within CA265_RS18185 at 4536.182 kb on + strand, within CA265_RS18185 at 4536.311 kb on - strand, within CA265_RS18185 at 4536.311 kb on - strand, within CA265_RS18185 at 4536.357 kb on - strand, within CA265_RS18185 at 4536.377 kb on - strand, within CA265_RS18185 at 4536.377 kb on - strand, within CA265_RS18185 at 4536.435 kb on - strand, within CA265_RS18185 at 4536.483 kb on - strand, within CA265_RS18185 at 4536.649 kb on - strand at 4536.669 kb on - strand at 4536.682 kb on - strand at 4536.682 kb on - strand at 4536.696 kb on + strand at 4536.713 kb on + strand at 4536.739 kb on - strand at 4536.746 kb on - strand at 4536.919 kb on + strand, within CA265_RS18190 at 4536.920 kb on - strand, within CA265_RS18190 at 4536.957 kb on - strand, within CA265_RS18190 at 4536.960 kb on + strand, within CA265_RS18190 at 4537.006 kb on + strand, within CA265_RS18190 at 4537.007 kb on - strand, within CA265_RS18190 at 4537.104 kb on + strand, within CA265_RS18190 at 4537.144 kb on + strand, within CA265_RS18190 at 4537.144 kb on + strand, within CA265_RS18190 at 4537.254 kb on + strand, within CA265_RS18190 at 4537.255 kb on - strand, within CA265_RS18190 at 4537.261 kb on + strand, within CA265_RS18190 at 4537.313 kb on - strand, within CA265_RS18190 at 4537.331 kb on + strand, within CA265_RS18190 at 4537.331 kb on + strand, within CA265_RS18190 at 4537.331 kb on + strand, within CA265_RS18190 at 4537.331 kb on + strand, within CA265_RS18190 at 4537.339 kb on - strand, within CA265_RS18190 at 4537.353 kb on - strand, within CA265_RS18190 at 4537.353 kb on - strand, within CA265_RS18190 at 4537.353 kb on - strand, within CA265_RS18190 at 4537.456 kb on - strand, within CA265_RS18190 at 4537.456 kb on - strand, within CA265_RS18190 at 4537.689 kb on - strand at 4537.712 kb on + strand at 4537.820 kb on - strand at 4537.820 kb on - strand at 4537.895 kb on - strand, within CA265_RS18195 at 4537.926 kb on - strand, within CA265_RS18195 at 4537.963 kb on + strand, within CA265_RS18195 at 4538.018 kb on + strand, within CA265_RS18195 at 4538.115 kb on - strand, within CA265_RS18195 at 4538.165 kb on - strand, within CA265_RS18195 at 4538.177 kb on - strand, within CA265_RS18195 at 4538.190 kb on + strand, within CA265_RS18195 at 4538.253 kb on - strand, within CA265_RS18195 at 4538.265 kb on - strand, within CA265_RS18195 at 4538.392 kb on - strand at 4538.467 kb on - strand at 4538.496 kb on - strand at 4538.499 kb on - strand at 4538.549 kb on - strand at 4538.551 kb on - strand at 4538.593 kb on + strand at 4538.595 kb on + strand at 4538.601 kb on + strand at 4538.601 kb on + strand at 4538.601 kb on + strand at 4538.602 kb on - strand at 4538.610 kb on - strand at 4538.627 kb on + strand at 4538.627 kb on + strand at 4538.648 kb on - strand at 4538.651 kb on - strand at 4538.657 kb on + strand at 4538.657 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 4,535,874 + +1.3 4,535,875 - -0.6 4,536,050 - CA265_RS18185 0.19 -1.8 4,536,062 - CA265_RS18185 0.21 -1.1 4,536,083 + CA265_RS18185 0.24 +1.9 4,536,182 + CA265_RS18185 0.36 +0.4 4,536,311 - CA265_RS18185 0.53 -0.2 4,536,311 - CA265_RS18185 0.53 -0.4 4,536,357 - CA265_RS18185 0.58 -0.7 4,536,377 - CA265_RS18185 0.61 +0.6 4,536,377 - CA265_RS18185 0.61 +0.8 4,536,435 - CA265_RS18185 0.68 +1.3 4,536,483 - CA265_RS18185 0.74 -0.1 4,536,649 - +0.4 4,536,669 - -1.0 4,536,682 - +0.9 4,536,682 - +0.7 4,536,696 + -0.9 4,536,713 + +0.7 4,536,739 - -1.3 4,536,746 - -0.5 4,536,919 + CA265_RS18190 0.12 -0.1 4,536,920 - CA265_RS18190 0.12 -1.7 4,536,957 - CA265_RS18190 0.16 -1.1 4,536,960 + CA265_RS18190 0.17 -1.7 4,537,006 + CA265_RS18190 0.22 -0.5 4,537,007 - CA265_RS18190 0.22 -1.2 4,537,104 + CA265_RS18190 0.33 -0.4 4,537,144 + CA265_RS18190 0.38 -1.1 4,537,144 + CA265_RS18190 0.38 +0.1 4,537,254 + CA265_RS18190 0.50 -2.3 4,537,255 - CA265_RS18190 0.50 -1.8 4,537,261 + CA265_RS18190 0.51 -0.7 4,537,313 - CA265_RS18190 0.57 -3.0 4,537,331 + CA265_RS18190 0.59 -1.3 4,537,331 + CA265_RS18190 0.59 -1.6 4,537,331 + CA265_RS18190 0.59 -2.0 4,537,331 + CA265_RS18190 0.59 -1.7 4,537,339 - CA265_RS18190 0.60 -2.0 4,537,353 - CA265_RS18190 0.62 -0.2 4,537,353 - CA265_RS18190 0.62 -0.1 4,537,353 - CA265_RS18190 0.62 -2.2 4,537,456 - CA265_RS18190 0.73 -1.3 4,537,456 - CA265_RS18190 0.73 -0.4 4,537,689 - -2.9 4,537,712 + +0.6 4,537,820 - -0.8 4,537,820 - +1.6 4,537,895 - CA265_RS18195 0.13 +0.6 4,537,926 - CA265_RS18195 0.18 -0.0 4,537,963 + CA265_RS18195 0.24 +0.1 4,538,018 + CA265_RS18195 0.33 +0.1 4,538,115 - CA265_RS18195 0.49 +0.7 4,538,165 - CA265_RS18195 0.58 +0.5 4,538,177 - CA265_RS18195 0.60 +1.5 4,538,190 + CA265_RS18195 0.62 -0.7 4,538,253 - CA265_RS18195 0.72 -1.0 4,538,265 - CA265_RS18195 0.74 +0.3 4,538,392 - +0.2 4,538,467 - -0.2 4,538,496 - -0.7 4,538,499 - -2.3 4,538,549 - -0.5 4,538,551 - +0.3 4,538,593 + +0.2 4,538,595 + -0.3 4,538,601 + +1.0 4,538,601 + +0.5 4,538,601 + -0.3 4,538,602 - +0.4 4,538,610 - +0.2 4,538,627 + -0.1 4,538,627 + -0.2 4,538,648 - -2.2 4,538,651 - -0.9 4,538,657 + -0.7 4,538,657 + +0.1
Or see this region's nucleotide sequence