Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS15100 and CA265_RS15105 are separated by 182 nucleotides CA265_RS15105 and CA265_RS15110 are separated by 109 nucleotides CA265_RS15110 and CA265_RS15115 are separated by 90 nucleotides CA265_RS15115 and CA265_RS15120 overlap by 4 nucleotides
CA265_RS15100: CA265_RS15100 - alpha/beta hydrolase, at 3,803,920 to 3,804,684
_RS15100
CA265_RS15105: CA265_RS15105 - glutamyl-tRNA amidotransferase, at 3,804,867 to 3,805,313
_RS15105
CA265_RS15110: CA265_RS15110 - NAD(P)-dependent oxidoreductase, at 3,805,423 to 3,806,181
_RS15110
CA265_RS15115: CA265_RS15115 - porin, at 3,806,272 to 3,807,069
_RS15115
CA265_RS15120: CA265_RS15120 - bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, at 3,807,066 to 3,807,800
_RS15120
Position (kb)
3805
3806
3807 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5
6
7 at 3804.685 kb on - strand at 3804.694 kb on - strand at 3804.694 kb on - strand at 3804.717 kb on - strand at 3804.829 kb on + strand at 3804.849 kb on - strand at 3804.868 kb on + strand at 3804.948 kb on + strand, within CA265_RS15105 at 3804.974 kb on + strand, within CA265_RS15105 at 3804.975 kb on - strand, within CA265_RS15105 at 3805.026 kb on - strand, within CA265_RS15105 at 3805.112 kb on - strand, within CA265_RS15105 at 3805.115 kb on + strand, within CA265_RS15105 at 3805.115 kb on + strand, within CA265_RS15105 at 3805.116 kb on - strand, within CA265_RS15105 at 3805.116 kb on - strand, within CA265_RS15105 at 3805.209 kb on - strand, within CA265_RS15105 at 3805.230 kb on + strand, within CA265_RS15105 at 3805.240 kb on - strand, within CA265_RS15105 at 3805.240 kb on - strand at 3805.296 kb on + strand at 3805.296 kb on + strand at 3805.297 kb on - strand at 3805.297 kb on - strand at 3805.326 kb on + strand at 3805.341 kb on + strand at 3805.366 kb on + strand at 3805.397 kb on + strand at 3805.426 kb on + strand at 3805.426 kb on + strand at 3805.476 kb on + strand at 3805.510 kb on + strand, within CA265_RS15110 at 3805.684 kb on - strand, within CA265_RS15110 at 3805.684 kb on - strand, within CA265_RS15110 at 3805.684 kb on - strand, within CA265_RS15110 at 3805.713 kb on + strand, within CA265_RS15110 at 3805.777 kb on - strand, within CA265_RS15110 at 3805.797 kb on + strand, within CA265_RS15110 at 3805.830 kb on - strand, within CA265_RS15110 at 3805.852 kb on - strand, within CA265_RS15110 at 3805.878 kb on + strand, within CA265_RS15110 at 3805.878 kb on + strand, within CA265_RS15110 at 3805.901 kb on - strand, within CA265_RS15110 at 3806.033 kb on + strand, within CA265_RS15110 at 3806.033 kb on + strand, within CA265_RS15110 at 3806.034 kb on - strand, within CA265_RS15110 at 3806.092 kb on + strand, within CA265_RS15110 at 3806.102 kb on + strand, within CA265_RS15110 at 3806.102 kb on + strand, within CA265_RS15110 at 3806.143 kb on - strand at 3806.164 kb on + strand at 3806.164 kb on + strand at 3806.204 kb on + strand at 3806.205 kb on - strand at 3806.206 kb on + strand at 3806.213 kb on + strand at 3806.237 kb on - strand at 3806.237 kb on - strand at 3806.250 kb on + strand at 3806.296 kb on + strand at 3806.317 kb on - strand at 3806.341 kb on - strand at 3806.343 kb on + strand at 3806.405 kb on + strand, within CA265_RS15115 at 3806.431 kb on - strand, within CA265_RS15115 at 3806.475 kb on + strand, within CA265_RS15115 at 3806.522 kb on - strand, within CA265_RS15115 at 3806.523 kb on + strand, within CA265_RS15115 at 3806.523 kb on + strand, within CA265_RS15115 at 3806.588 kb on - strand, within CA265_RS15115 at 3806.638 kb on + strand, within CA265_RS15115 at 3806.638 kb on + strand, within CA265_RS15115 at 3806.675 kb on - strand, within CA265_RS15115 at 3806.675 kb on - strand, within CA265_RS15115 at 3806.675 kb on - strand, within CA265_RS15115 at 3806.705 kb on + strand, within CA265_RS15115 at 3806.706 kb on - strand, within CA265_RS15115 at 3806.776 kb on - strand, within CA265_RS15115 at 3806.776 kb on - strand, within CA265_RS15115 at 3806.831 kb on - strand, within CA265_RS15115 at 3806.831 kb on - strand, within CA265_RS15115 at 3806.831 kb on - strand, within CA265_RS15115 at 3806.926 kb on - strand, within CA265_RS15115 at 3806.949 kb on + strand, within CA265_RS15115 at 3806.951 kb on + strand, within CA265_RS15115 at 3807.025 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 3,804,685 - +1.9 3,804,694 - +0.3 3,804,694 - +1.7 3,804,717 - -1.3 3,804,829 + +2.8 3,804,849 - -2.9 3,804,868 + +3.0 3,804,948 + CA265_RS15105 0.18 +4.0 3,804,974 + CA265_RS15105 0.24 +4.0 3,804,975 - CA265_RS15105 0.24 -2.6 3,805,026 - CA265_RS15105 0.36 -1.6 3,805,112 - CA265_RS15105 0.55 -0.1 3,805,115 + CA265_RS15105 0.55 +3.3 3,805,115 + CA265_RS15105 0.55 +4.0 3,805,116 - CA265_RS15105 0.56 -1.5 3,805,116 - CA265_RS15105 0.56 -3.6 3,805,209 - CA265_RS15105 0.77 -3.8 3,805,230 + CA265_RS15105 0.81 +3.1 3,805,240 - CA265_RS15105 0.83 -1.2 3,805,240 - +0.1 3,805,296 + +3.2 3,805,296 + +3.5 3,805,297 - -1.5 3,805,297 - -2.2 3,805,326 + +3.7 3,805,341 + +3.5 3,805,366 + +4.2 3,805,397 + +3.6 3,805,426 + +3.5 3,805,426 + +2.7 3,805,476 + +3.5 3,805,510 + CA265_RS15110 0.11 +2.6 3,805,684 - CA265_RS15110 0.34 -3.6 3,805,684 - CA265_RS15110 0.34 -2.4 3,805,684 - CA265_RS15110 0.34 -0.9 3,805,713 + CA265_RS15110 0.38 +3.4 3,805,777 - CA265_RS15110 0.47 -0.2 3,805,797 + CA265_RS15110 0.49 +4.0 3,805,830 - CA265_RS15110 0.54 -2.4 3,805,852 - CA265_RS15110 0.57 -1.2 3,805,878 + CA265_RS15110 0.60 +3.4 3,805,878 + CA265_RS15110 0.60 +3.0 3,805,901 - CA265_RS15110 0.63 -4.1 3,806,033 + CA265_RS15110 0.80 +3.1 3,806,033 + CA265_RS15110 0.80 +3.1 3,806,034 - CA265_RS15110 0.81 -1.2 3,806,092 + CA265_RS15110 0.88 +2.8 3,806,102 + CA265_RS15110 0.89 +3.1 3,806,102 + CA265_RS15110 0.89 +3.7 3,806,143 - -1.8 3,806,164 + +3.6 3,806,164 + +2.0 3,806,204 + +2.8 3,806,205 - -2.9 3,806,206 + +2.4 3,806,213 + +2.3 3,806,237 - -0.9 3,806,237 - -1.5 3,806,250 + +3.4 3,806,296 + +7.2 3,806,317 - -0.6 3,806,341 - -1.0 3,806,343 + +6.7 3,806,405 + CA265_RS15115 0.17 +6.6 3,806,431 - CA265_RS15115 0.20 +0.5 3,806,475 + CA265_RS15115 0.25 +6.4 3,806,522 - CA265_RS15115 0.31 +0.3 3,806,523 + CA265_RS15115 0.31 +6.3 3,806,523 + CA265_RS15115 0.31 +3.3 3,806,588 - CA265_RS15115 0.40 +0.1 3,806,638 + CA265_RS15115 0.46 +6.0 3,806,638 + CA265_RS15115 0.46 +7.3 3,806,675 - CA265_RS15115 0.51 +1.0 3,806,675 - CA265_RS15115 0.51 -1.2 3,806,675 - CA265_RS15115 0.51 +0.5 3,806,705 + CA265_RS15115 0.54 +6.2 3,806,706 - CA265_RS15115 0.54 +0.2 3,806,776 - CA265_RS15115 0.63 +2.1 3,806,776 - CA265_RS15115 0.63 +1.4 3,806,831 - CA265_RS15115 0.70 +1.1 3,806,831 - CA265_RS15115 0.70 +1.6 3,806,831 - CA265_RS15115 0.70 +1.9 3,806,926 - CA265_RS15115 0.82 +2.7 3,806,949 + CA265_RS15115 0.85 +5.7 3,806,951 + CA265_RS15115 0.85 +6.1 3,807,025 - +3.3
Or see this region's nucleotide sequence