Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS15025 and CA265_RS15030 are separated by 239 nucleotides CA265_RS15030 and CA265_RS15035 overlap by 4 nucleotides CA265_RS15035 and CA265_RS15040 are separated by 153 nucleotides CA265_RS15040 and CA265_RS15045 are separated by 64 nucleotides
CA265_RS15025: CA265_RS15025 - GMP synthase (glutamine-hydrolyzing), at 3,787,514 to 3,789,043
_RS15025
CA265_RS15030: CA265_RS15030 - PIN domain nuclease, at 3,789,283 to 3,789,681
_RS15030
CA265_RS15035: CA265_RS15035 - prevent-host-death protein, at 3,789,678 to 3,789,905
_RS15035
CA265_RS15040: CA265_RS15040 - cytidine deaminase, at 3,790,059 to 3,790,517
_RS15040
CA265_RS15045: CA265_RS15045 - MFS transporter, at 3,790,582 to 3,792,189
_RS15045
Position (kb)
3789
3790 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3789.099 kb on + strand at 3789.100 kb on - strand at 3789.173 kb on + strand at 3789.237 kb on + strand at 3789.257 kb on - strand at 3789.321 kb on + strand at 3789.343 kb on + strand, within CA265_RS15030 at 3789.416 kb on - strand, within CA265_RS15030 at 3789.416 kb on - strand, within CA265_RS15030 at 3789.420 kb on + strand, within CA265_RS15030 at 3789.498 kb on + strand, within CA265_RS15030 at 3789.499 kb on - strand, within CA265_RS15030 at 3789.561 kb on + strand, within CA265_RS15030 at 3789.562 kb on - strand, within CA265_RS15030 at 3789.571 kb on - strand, within CA265_RS15030 at 3789.641 kb on - strand, within CA265_RS15030 at 3789.641 kb on - strand, within CA265_RS15030 at 3789.663 kb on + strand at 3789.740 kb on + strand, within CA265_RS15035 at 3789.839 kb on + strand, within CA265_RS15035 at 3789.991 kb on + strand at 3790.133 kb on + strand, within CA265_RS15040 at 3790.157 kb on - strand, within CA265_RS15040 at 3790.160 kb on - strand, within CA265_RS15040 at 3790.160 kb on - strand, within CA265_RS15040 at 3790.225 kb on + strand, within CA265_RS15040 at 3790.225 kb on + strand, within CA265_RS15040 at 3790.227 kb on + strand, within CA265_RS15040 at 3790.267 kb on + strand, within CA265_RS15040 at 3790.293 kb on + strand, within CA265_RS15040 at 3790.320 kb on - strand, within CA265_RS15040 at 3790.361 kb on + strand, within CA265_RS15040 at 3790.385 kb on + strand, within CA265_RS15040 at 3790.388 kb on - strand, within CA265_RS15040 at 3790.388 kb on - strand, within CA265_RS15040 at 3790.400 kb on + strand, within CA265_RS15040 at 3790.485 kb on - strand at 3790.489 kb on - strand at 3790.620 kb on + strand at 3790.621 kb on - strand at 3790.621 kb on - strand at 3790.670 kb on + strand at 3790.670 kb on + strand at 3790.697 kb on + strand at 3790.773 kb on + strand, within CA265_RS15045 at 3790.851 kb on + strand, within CA265_RS15045 at 3790.851 kb on + strand, within CA265_RS15045 at 3790.851 kb on + strand, within CA265_RS15045 at 3790.851 kb on + strand, within CA265_RS15045 at 3790.851 kb on + strand, within CA265_RS15045 at 3790.852 kb on - strand, within CA265_RS15045 at 3790.852 kb on - strand, within CA265_RS15045 at 3790.861 kb on + strand, within CA265_RS15045
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 3,789,099 + -1.1 3,789,100 - -0.4 3,789,173 + -1.1 3,789,237 + -0.8 3,789,257 - -0.5 3,789,321 + -0.9 3,789,343 + CA265_RS15030 0.15 +0.6 3,789,416 - CA265_RS15030 0.33 +1.0 3,789,416 - CA265_RS15030 0.33 -0.8 3,789,420 + CA265_RS15030 0.34 -2.5 3,789,498 + CA265_RS15030 0.54 +0.3 3,789,499 - CA265_RS15030 0.54 -0.8 3,789,561 + CA265_RS15030 0.70 +0.1 3,789,562 - CA265_RS15030 0.70 +0.0 3,789,571 - CA265_RS15030 0.72 -0.1 3,789,641 - CA265_RS15030 0.90 -0.8 3,789,641 - CA265_RS15030 0.90 +0.5 3,789,663 + +0.1 3,789,740 + CA265_RS15035 0.27 -0.7 3,789,839 + CA265_RS15035 0.71 -1.1 3,789,991 + -1.6 3,790,133 + CA265_RS15040 0.16 -2.4 3,790,157 - CA265_RS15040 0.21 -0.5 3,790,160 - CA265_RS15040 0.22 -1.4 3,790,160 - CA265_RS15040 0.22 +0.2 3,790,225 + CA265_RS15040 0.36 -2.2 3,790,225 + CA265_RS15040 0.36 -1.9 3,790,227 + CA265_RS15040 0.37 -2.3 3,790,267 + CA265_RS15040 0.45 -1.7 3,790,293 + CA265_RS15040 0.51 -1.7 3,790,320 - CA265_RS15040 0.57 -0.6 3,790,361 + CA265_RS15040 0.66 -2.6 3,790,385 + CA265_RS15040 0.71 -1.2 3,790,388 - CA265_RS15040 0.72 +0.6 3,790,388 - CA265_RS15040 0.72 +0.6 3,790,400 + CA265_RS15040 0.74 -1.6 3,790,485 - -1.1 3,790,489 - -1.4 3,790,620 + -2.9 3,790,621 - -2.4 3,790,621 - -2.1 3,790,670 + -3.1 3,790,670 + -2.7 3,790,697 + -1.2 3,790,773 + CA265_RS15045 0.12 -1.8 3,790,851 + CA265_RS15045 0.17 -2.9 3,790,851 + CA265_RS15045 0.17 -2.8 3,790,851 + CA265_RS15045 0.17 -1.8 3,790,851 + CA265_RS15045 0.17 -2.0 3,790,851 + CA265_RS15045 0.17 -1.8 3,790,852 - CA265_RS15045 0.17 -2.5 3,790,852 - CA265_RS15045 0.17 -2.1 3,790,861 + CA265_RS15045 0.17 -2.2
Or see this region's nucleotide sequence