Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS14240 and CA265_RS14245 are separated by 804 nucleotides CA265_RS14245 and CA265_RS14250 are separated by 97 nucleotides
CA265_RS14240: CA265_RS14240 - G-D-S-L family lipolytic protein, at 3,593,409 to 3,595,523
_RS14240
CA265_RS14245: CA265_RS14245 - hypothetical protein, at 3,596,328 to 3,597,839
_RS14245
CA265_RS14250: CA265_RS14250 - hypothetical protein, at 3,597,937 to 3,600,195
_RS14250
Position (kb)
3596
3597
3598 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3595.500 kb on - strand at 3595.569 kb on + strand at 3595.570 kb on - strand at 3595.659 kb on - strand at 3595.703 kb on + strand at 3595.703 kb on + strand at 3595.721 kb on + strand at 3595.790 kb on + strand at 3595.946 kb on - strand at 3596.000 kb on + strand at 3596.112 kb on - strand at 3596.135 kb on - strand at 3596.184 kb on - strand at 3596.194 kb on + strand at 3596.250 kb on - strand at 3596.258 kb on - strand at 3596.271 kb on + strand at 3596.337 kb on + strand at 3596.440 kb on - strand at 3596.475 kb on + strand at 3596.491 kb on + strand, within CA265_RS14245 at 3596.523 kb on + strand, within CA265_RS14245 at 3596.525 kb on + strand, within CA265_RS14245 at 3596.526 kb on - strand, within CA265_RS14245 at 3596.775 kb on - strand, within CA265_RS14245 at 3596.808 kb on - strand, within CA265_RS14245 at 3596.819 kb on + strand, within CA265_RS14245 at 3596.833 kb on - strand, within CA265_RS14245 at 3596.900 kb on + strand, within CA265_RS14245 at 3596.928 kb on + strand, within CA265_RS14245 at 3597.011 kb on + strand, within CA265_RS14245 at 3597.011 kb on + strand, within CA265_RS14245 at 3597.028 kb on + strand, within CA265_RS14245 at 3597.122 kb on + strand, within CA265_RS14245 at 3597.180 kb on - strand, within CA265_RS14245 at 3597.304 kb on - strand, within CA265_RS14245 at 3597.313 kb on + strand, within CA265_RS14245 at 3597.321 kb on + strand, within CA265_RS14245 at 3597.325 kb on - strand, within CA265_RS14245 at 3597.354 kb on - strand, within CA265_RS14245 at 3597.413 kb on - strand, within CA265_RS14245 at 3597.457 kb on - strand, within CA265_RS14245 at 3597.457 kb on - strand, within CA265_RS14245 at 3597.503 kb on - strand, within CA265_RS14245 at 3597.503 kb on - strand, within CA265_RS14245 at 3597.560 kb on + strand, within CA265_RS14245 at 3597.566 kb on - strand, within CA265_RS14245 at 3597.566 kb on - strand, within CA265_RS14245 at 3597.570 kb on - strand, within CA265_RS14245 at 3597.677 kb on + strand, within CA265_RS14245 at 3597.678 kb on - strand, within CA265_RS14245 at 3597.681 kb on + strand, within CA265_RS14245 at 3597.732 kb on + strand at 3597.768 kb on + strand at 3597.768 kb on + strand at 3597.796 kb on - strand at 3597.803 kb on + strand at 3597.804 kb on - strand at 3597.804 kb on - strand at 3597.821 kb on + strand at 3597.822 kb on - strand at 3597.852 kb on + strand at 3597.853 kb on - strand at 3597.856 kb on + strand at 3597.868 kb on - strand at 3598.047 kb on - strand at 3598.061 kb on - strand at 3598.075 kb on + strand at 3598.075 kb on + strand at 3598.216 kb on + strand, within CA265_RS14250 at 3598.216 kb on + strand, within CA265_RS14250 at 3598.217 kb on - strand, within CA265_RS14250 at 3598.218 kb on + strand, within CA265_RS14250 at 3598.218 kb on + strand, within CA265_RS14250 at 3598.218 kb on + strand, within CA265_RS14250 at 3598.219 kb on - strand, within CA265_RS14250 at 3598.219 kb on - strand, within CA265_RS14250 at 3598.219 kb on - strand, within CA265_RS14250 at 3598.221 kb on - strand, within CA265_RS14250 at 3598.424 kb on + strand, within CA265_RS14250 at 3598.443 kb on + strand, within CA265_RS14250 at 3598.516 kb on - strand, within CA265_RS14250 at 3598.564 kb on - strand, within CA265_RS14250 at 3598.564 kb on - strand, within CA265_RS14250 at 3598.564 kb on - strand, within CA265_RS14250 at 3598.566 kb on - strand, within CA265_RS14250 at 3598.566 kb on - strand, within CA265_RS14250 at 3598.599 kb on + strand, within CA265_RS14250 at 3598.600 kb on - strand, within CA265_RS14250 at 3598.613 kb on - strand, within CA265_RS14250 at 3598.708 kb on + strand, within CA265_RS14250 at 3598.709 kb on - strand, within CA265_RS14250 at 3598.726 kb on - strand, within CA265_RS14250 at 3598.782 kb on + strand, within CA265_RS14250 at 3598.782 kb on + strand, within CA265_RS14250 at 3598.785 kb on + strand, within CA265_RS14250 at 3598.786 kb on - strand, within CA265_RS14250 at 3598.792 kb on - strand, within CA265_RS14250 at 3598.799 kb on + strand, within CA265_RS14250 at 3598.800 kb on - strand, within CA265_RS14250 at 3598.810 kb on - strand, within CA265_RS14250
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 3,595,500 - +0.8 3,595,569 + -2.0 3,595,570 - -0.7 3,595,659 - -1.0 3,595,703 + -1.0 3,595,703 + -0.8 3,595,721 + -1.6 3,595,790 + -0.0 3,595,946 - +0.2 3,596,000 + +0.4 3,596,112 - +0.0 3,596,135 - -0.1 3,596,184 - +0.3 3,596,194 + +0.6 3,596,250 - -1.0 3,596,258 - +0.5 3,596,271 + -0.7 3,596,337 + +0.2 3,596,440 - -0.6 3,596,475 + -0.5 3,596,491 + CA265_RS14245 0.11 -0.4 3,596,523 + CA265_RS14245 0.13 +0.9 3,596,525 + CA265_RS14245 0.13 -0.3 3,596,526 - CA265_RS14245 0.13 -1.7 3,596,775 - CA265_RS14245 0.30 +0.2 3,596,808 - CA265_RS14245 0.32 -0.2 3,596,819 + CA265_RS14245 0.32 +0.9 3,596,833 - CA265_RS14245 0.33 +1.8 3,596,900 + CA265_RS14245 0.38 -0.2 3,596,928 + CA265_RS14245 0.40 -1.0 3,597,011 + CA265_RS14245 0.45 -0.9 3,597,011 + CA265_RS14245 0.45 -0.0 3,597,028 + CA265_RS14245 0.46 +0.0 3,597,122 + CA265_RS14245 0.53 +0.0 3,597,180 - CA265_RS14245 0.56 -0.4 3,597,304 - CA265_RS14245 0.65 -0.0 3,597,313 + CA265_RS14245 0.65 -0.5 3,597,321 + CA265_RS14245 0.66 -0.6 3,597,325 - CA265_RS14245 0.66 -0.6 3,597,354 - CA265_RS14245 0.68 -0.1 3,597,413 - CA265_RS14245 0.72 -0.0 3,597,457 - CA265_RS14245 0.75 -0.0 3,597,457 - CA265_RS14245 0.75 +0.2 3,597,503 - CA265_RS14245 0.78 -1.2 3,597,503 - CA265_RS14245 0.78 -0.4 3,597,560 + CA265_RS14245 0.81 +0.2 3,597,566 - CA265_RS14245 0.82 +0.8 3,597,566 - CA265_RS14245 0.82 -0.7 3,597,570 - CA265_RS14245 0.82 +0.9 3,597,677 + CA265_RS14245 0.89 +0.7 3,597,678 - CA265_RS14245 0.89 -0.2 3,597,681 + CA265_RS14245 0.89 +1.1 3,597,732 + +0.3 3,597,768 + -0.6 3,597,768 + +1.5 3,597,796 - +0.4 3,597,803 + -1.6 3,597,804 - +2.1 3,597,804 - +0.6 3,597,821 + -0.5 3,597,822 - -1.1 3,597,852 + -0.7 3,597,853 - +1.2 3,597,856 + -0.9 3,597,868 - -0.4 3,598,047 - +0.6 3,598,061 - -1.2 3,598,075 + -0.4 3,598,075 + +0.5 3,598,216 + CA265_RS14250 0.12 +0.3 3,598,216 + CA265_RS14250 0.12 +0.3 3,598,217 - CA265_RS14250 0.12 -1.3 3,598,218 + CA265_RS14250 0.12 +0.4 3,598,218 + CA265_RS14250 0.12 -0.2 3,598,218 + CA265_RS14250 0.12 -0.4 3,598,219 - CA265_RS14250 0.12 -2.9 3,598,219 - CA265_RS14250 0.12 -0.1 3,598,219 - CA265_RS14250 0.12 +0.6 3,598,221 - CA265_RS14250 0.13 -0.2 3,598,424 + CA265_RS14250 0.22 +0.2 3,598,443 + CA265_RS14250 0.22 -0.4 3,598,516 - CA265_RS14250 0.26 -1.4 3,598,564 - CA265_RS14250 0.28 +0.7 3,598,564 - CA265_RS14250 0.28 +0.3 3,598,564 - CA265_RS14250 0.28 -1.5 3,598,566 - CA265_RS14250 0.28 -2.0 3,598,566 - CA265_RS14250 0.28 +0.3 3,598,599 + CA265_RS14250 0.29 +0.1 3,598,600 - CA265_RS14250 0.29 -0.0 3,598,613 - CA265_RS14250 0.30 +0.6 3,598,708 + CA265_RS14250 0.34 -0.9 3,598,709 - CA265_RS14250 0.34 +0.5 3,598,726 - CA265_RS14250 0.35 -0.7 3,598,782 + CA265_RS14250 0.37 -1.9 3,598,782 + CA265_RS14250 0.37 -0.4 3,598,785 + CA265_RS14250 0.38 +0.5 3,598,786 - CA265_RS14250 0.38 +0.6 3,598,792 - CA265_RS14250 0.38 +0.2 3,598,799 + CA265_RS14250 0.38 +1.1 3,598,800 - CA265_RS14250 0.38 +0.0 3,598,810 - CA265_RS14250 0.39 -0.2
Or see this region's nucleotide sequence