Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS14055 and CA265_RS14060 are separated by 70 nucleotides CA265_RS14060 and CA265_RS14065 are separated by 170 nucleotides
CA265_RS14055: CA265_RS14055 - acyl-CoA thioesterase, at 3,536,432 to 3,536,953
_RS14055
CA265_RS14060: CA265_RS14060 - sodium:proton antiporter, at 3,537,024 to 3,538,205
_RS14060
CA265_RS14065: CA265_RS14065 - hypothetical protein, at 3,538,376 to 3,538,939
_RS14065
Position (kb)
3537
3538
3539 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3536.092 kb on + strand at 3536.094 kb on + strand at 3536.095 kb on - strand at 3536.095 kb on - strand at 3536.095 kb on - strand at 3536.099 kb on - strand at 3536.099 kb on - strand at 3536.102 kb on + strand at 3536.103 kb on - strand at 3536.180 kb on + strand at 3536.180 kb on + strand at 3536.267 kb on + strand at 3536.342 kb on + strand at 3536.343 kb on - strand at 3536.370 kb on + strand at 3536.506 kb on + strand, within CA265_RS14055 at 3536.511 kb on + strand, within CA265_RS14055 at 3536.511 kb on + strand, within CA265_RS14055 at 3536.512 kb on - strand, within CA265_RS14055 at 3536.652 kb on + strand, within CA265_RS14055 at 3536.653 kb on - strand, within CA265_RS14055 at 3536.653 kb on - strand, within CA265_RS14055 at 3536.686 kb on + strand, within CA265_RS14055 at 3536.709 kb on + strand, within CA265_RS14055 at 3536.710 kb on - strand, within CA265_RS14055 at 3536.765 kb on + strand, within CA265_RS14055 at 3536.766 kb on - strand, within CA265_RS14055 at 3536.766 kb on - strand, within CA265_RS14055 at 3536.768 kb on + strand, within CA265_RS14055 at 3536.769 kb on - strand, within CA265_RS14055 at 3536.769 kb on - strand at 3536.771 kb on - strand, within CA265_RS14055 at 3536.880 kb on + strand, within CA265_RS14055 at 3536.945 kb on - strand at 3536.963 kb on + strand at 3536.964 kb on - strand at 3537.034 kb on - strand at 3537.034 kb on - strand at 3537.034 kb on - strand at 3537.044 kb on + strand at 3537.098 kb on + strand at 3537.121 kb on + strand at 3537.122 kb on - strand at 3537.198 kb on - strand, within CA265_RS14060 at 3537.220 kb on + strand, within CA265_RS14060 at 3537.221 kb on - strand, within CA265_RS14060 at 3537.221 kb on - strand, within CA265_RS14060 at 3537.264 kb on + strand, within CA265_RS14060 at 3537.314 kb on - strand, within CA265_RS14060 at 3537.314 kb on - strand, within CA265_RS14060 at 3537.333 kb on - strand, within CA265_RS14060 at 3537.354 kb on - strand, within CA265_RS14060 at 3537.354 kb on - strand, within CA265_RS14060 at 3537.379 kb on + strand, within CA265_RS14060 at 3537.379 kb on + strand, within CA265_RS14060 at 3537.389 kb on + strand, within CA265_RS14060 at 3537.389 kb on + strand, within CA265_RS14060 at 3537.402 kb on - strand, within CA265_RS14060 at 3537.483 kb on + strand, within CA265_RS14060 at 3537.515 kb on + strand, within CA265_RS14060 at 3537.581 kb on - strand, within CA265_RS14060 at 3537.581 kb on - strand, within CA265_RS14060 at 3537.585 kb on - strand, within CA265_RS14060 at 3537.604 kb on + strand, within CA265_RS14060 at 3537.633 kb on + strand, within CA265_RS14060 at 3537.634 kb on - strand, within CA265_RS14060 at 3537.677 kb on + strand, within CA265_RS14060 at 3537.677 kb on + strand, within CA265_RS14060 at 3537.692 kb on - strand, within CA265_RS14060 at 3537.718 kb on - strand, within CA265_RS14060 at 3537.908 kb on + strand, within CA265_RS14060 at 3537.908 kb on + strand, within CA265_RS14060 at 3537.917 kb on + strand, within CA265_RS14060 at 3537.917 kb on + strand, within CA265_RS14060 at 3537.918 kb on - strand, within CA265_RS14060 at 3537.918 kb on - strand, within CA265_RS14060 at 3537.918 kb on - strand, within CA265_RS14060 at 3537.919 kb on + strand, within CA265_RS14060 at 3537.919 kb on + strand, within CA265_RS14060 at 3537.919 kb on + strand, within CA265_RS14060 at 3537.920 kb on - strand, within CA265_RS14060 at 3537.920 kb on - strand, within CA265_RS14060 at 3537.920 kb on - strand, within CA265_RS14060 at 3537.923 kb on + strand, within CA265_RS14060 at 3538.037 kb on - strand, within CA265_RS14060 at 3538.080 kb on - strand, within CA265_RS14060 at 3538.171 kb on + strand at 3538.181 kb on + strand at 3538.232 kb on + strand at 3538.236 kb on - strand at 3538.302 kb on - strand at 3538.385 kb on - strand at 3538.428 kb on - strand at 3538.449 kb on + strand, within CA265_RS14065 at 3538.449 kb on + strand, within CA265_RS14065 at 3538.453 kb on + strand, within CA265_RS14065 at 3538.454 kb on - strand, within CA265_RS14065 at 3538.458 kb on + strand, within CA265_RS14065 at 3538.504 kb on + strand, within CA265_RS14065 at 3538.537 kb on + strand, within CA265_RS14065 at 3538.537 kb on + strand, within CA265_RS14065 at 3538.542 kb on - strand, within CA265_RS14065 at 3538.664 kb on - strand, within CA265_RS14065 at 3538.739 kb on + strand, within CA265_RS14065 at 3538.740 kb on - strand, within CA265_RS14065 at 3538.816 kb on + strand, within CA265_RS14065 at 3538.867 kb on - strand, within CA265_RS14065 at 3538.922 kb on - strand at 3538.962 kb on - strand at 3538.967 kb on - strand at 3539.038 kb on - strand at 3539.141 kb on - strand at 3539.145 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 3,536,092 + +0.8 3,536,094 + -1.2 3,536,095 - +0.5 3,536,095 - -0.9 3,536,095 - -0.8 3,536,099 - +0.9 3,536,099 - -0.1 3,536,102 + +0.2 3,536,103 - -0.6 3,536,180 + +0.8 3,536,180 + -0.5 3,536,267 + -0.8 3,536,342 + -0.7 3,536,343 - +0.3 3,536,370 + -0.8 3,536,506 + CA265_RS14055 0.14 -0.2 3,536,511 + CA265_RS14055 0.15 -0.1 3,536,511 + CA265_RS14055 0.15 +0.5 3,536,512 - CA265_RS14055 0.15 +0.1 3,536,652 + CA265_RS14055 0.42 -0.4 3,536,653 - CA265_RS14055 0.42 -1.9 3,536,653 - CA265_RS14055 0.42 -0.8 3,536,686 + CA265_RS14055 0.49 -0.4 3,536,709 + CA265_RS14055 0.53 -0.6 3,536,710 - CA265_RS14055 0.53 -1.5 3,536,765 + CA265_RS14055 0.64 -0.4 3,536,766 - CA265_RS14055 0.64 +0.2 3,536,766 - CA265_RS14055 0.64 +0.9 3,536,768 + CA265_RS14055 0.64 +1.0 3,536,769 - CA265_RS14055 0.65 +0.9 3,536,769 - +2.1 3,536,771 - CA265_RS14055 0.65 +0.0 3,536,880 + CA265_RS14055 0.86 -0.8 3,536,945 - -0.3 3,536,963 + -1.2 3,536,964 - +2.1 3,537,034 - -0.2 3,537,034 - -0.2 3,537,034 - -0.8 3,537,044 + -0.1 3,537,098 + -0.4 3,537,121 + -0.1 3,537,122 - -0.1 3,537,198 - CA265_RS14060 0.15 +0.8 3,537,220 + CA265_RS14060 0.17 +0.0 3,537,221 - CA265_RS14060 0.17 -0.3 3,537,221 - CA265_RS14060 0.17 +1.2 3,537,264 + CA265_RS14060 0.20 -1.5 3,537,314 - CA265_RS14060 0.25 -0.3 3,537,314 - CA265_RS14060 0.25 -1.2 3,537,333 - CA265_RS14060 0.26 +0.7 3,537,354 - CA265_RS14060 0.28 +0.5 3,537,354 - CA265_RS14060 0.28 -0.5 3,537,379 + CA265_RS14060 0.30 -0.4 3,537,379 + CA265_RS14060 0.30 -0.5 3,537,389 + CA265_RS14060 0.31 -0.9 3,537,389 + CA265_RS14060 0.31 -1.3 3,537,402 - CA265_RS14060 0.32 -1.4 3,537,483 + CA265_RS14060 0.39 -0.0 3,537,515 + CA265_RS14060 0.42 -0.8 3,537,581 - CA265_RS14060 0.47 +0.9 3,537,581 - CA265_RS14060 0.47 -1.4 3,537,585 - CA265_RS14060 0.47 -0.7 3,537,604 + CA265_RS14060 0.49 +0.6 3,537,633 + CA265_RS14060 0.52 -0.4 3,537,634 - CA265_RS14060 0.52 +0.7 3,537,677 + CA265_RS14060 0.55 -0.2 3,537,677 + CA265_RS14060 0.55 -0.1 3,537,692 - CA265_RS14060 0.57 -0.4 3,537,718 - CA265_RS14060 0.59 -0.1 3,537,908 + CA265_RS14060 0.75 -0.4 3,537,908 + CA265_RS14060 0.75 -0.1 3,537,917 + CA265_RS14060 0.76 -0.4 3,537,917 + CA265_RS14060 0.76 -0.8 3,537,918 - CA265_RS14060 0.76 -1.1 3,537,918 - CA265_RS14060 0.76 -0.1 3,537,918 - CA265_RS14060 0.76 -0.2 3,537,919 + CA265_RS14060 0.76 -1.2 3,537,919 + CA265_RS14060 0.76 -0.3 3,537,919 + CA265_RS14060 0.76 -0.9 3,537,920 - CA265_RS14060 0.76 -1.8 3,537,920 - CA265_RS14060 0.76 -0.7 3,537,920 - CA265_RS14060 0.76 +0.9 3,537,923 + CA265_RS14060 0.76 -0.6 3,538,037 - CA265_RS14060 0.86 -0.1 3,538,080 - CA265_RS14060 0.89 -0.1 3,538,171 + +0.4 3,538,181 + -0.7 3,538,232 + +0.0 3,538,236 - +0.5 3,538,302 - -0.2 3,538,385 - +0.7 3,538,428 - -0.8 3,538,449 + CA265_RS14065 0.13 -1.3 3,538,449 + CA265_RS14065 0.13 -0.9 3,538,453 + CA265_RS14065 0.14 +0.3 3,538,454 - CA265_RS14065 0.14 -1.0 3,538,458 + CA265_RS14065 0.15 -1.0 3,538,504 + CA265_RS14065 0.23 -1.0 3,538,537 + CA265_RS14065 0.29 -0.6 3,538,537 + CA265_RS14065 0.29 -1.0 3,538,542 - CA265_RS14065 0.29 -0.4 3,538,664 - CA265_RS14065 0.51 -0.7 3,538,739 + CA265_RS14065 0.64 -0.1 3,538,740 - CA265_RS14065 0.65 +0.1 3,538,816 + CA265_RS14065 0.78 -1.2 3,538,867 - CA265_RS14065 0.87 -0.1 3,538,922 - -3.3 3,538,962 - +0.1 3,538,967 - +0.6 3,539,038 - +0.8 3,539,141 - +0.7 3,539,145 - +0.5
Or see this region's nucleotide sequence