Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS14045 and CA265_RS14050 are separated by 237 nucleotides CA265_RS14050 and CA265_RS14055 are separated by 749 nucleotides
CA265_RS14045: CA265_RS14045 - xylose isomerase, at 3,533,031 to 3,533,957
_RS14045
CA265_RS14050: CA265_RS14050 - alpha-amylase, at 3,534,195 to 3,535,682
_RS14050
CA265_RS14055: CA265_RS14055 - acyl-CoA thioesterase, at 3,536,432 to 3,536,953
_RS14055
Position (kb)
3534
3535
3536 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3533.232 kb on + strand, within CA265_RS14045 at 3533.311 kb on + strand, within CA265_RS14045 at 3533.312 kb on - strand, within CA265_RS14045 at 3533.381 kb on - strand, within CA265_RS14045 at 3533.408 kb on + strand, within CA265_RS14045 at 3533.409 kb on - strand, within CA265_RS14045 at 3533.438 kb on - strand, within CA265_RS14045 at 3533.447 kb on + strand, within CA265_RS14045 at 3533.456 kb on + strand, within CA265_RS14045 at 3533.457 kb on - strand, within CA265_RS14045 at 3533.476 kb on + strand, within CA265_RS14045 at 3533.531 kb on - strand, within CA265_RS14045 at 3533.643 kb on + strand, within CA265_RS14045 at 3533.693 kb on + strand, within CA265_RS14045 at 3533.693 kb on + strand, within CA265_RS14045 at 3533.694 kb on - strand, within CA265_RS14045 at 3533.694 kb on - strand, within CA265_RS14045 at 3533.697 kb on + strand, within CA265_RS14045 at 3533.697 kb on + strand, within CA265_RS14045 at 3533.711 kb on + strand, within CA265_RS14045 at 3533.726 kb on - strand, within CA265_RS14045 at 3533.763 kb on + strand, within CA265_RS14045 at 3533.764 kb on - strand, within CA265_RS14045 at 3533.822 kb on + strand, within CA265_RS14045 at 3533.858 kb on + strand, within CA265_RS14045 at 3533.859 kb on - strand, within CA265_RS14045 at 3533.859 kb on - strand, within CA265_RS14045 at 3533.914 kb on + strand at 3533.915 kb on - strand at 3533.915 kb on - strand at 3533.915 kb on - strand at 3533.945 kb on + strand at 3534.049 kb on - strand at 3534.086 kb on + strand at 3534.124 kb on - strand at 3534.181 kb on - strand at 3534.223 kb on - strand at 3534.288 kb on - strand at 3534.434 kb on + strand, within CA265_RS14050 at 3534.435 kb on - strand, within CA265_RS14050 at 3534.435 kb on - strand, within CA265_RS14050 at 3534.488 kb on + strand, within CA265_RS14050 at 3534.609 kb on + strand, within CA265_RS14050 at 3534.610 kb on - strand, within CA265_RS14050 at 3534.626 kb on + strand, within CA265_RS14050 at 3534.626 kb on + strand, within CA265_RS14050 at 3534.631 kb on + strand, within CA265_RS14050 at 3534.699 kb on - strand, within CA265_RS14050 at 3534.800 kb on + strand, within CA265_RS14050 at 3534.903 kb on - strand, within CA265_RS14050 at 3534.948 kb on + strand, within CA265_RS14050 at 3534.975 kb on - strand, within CA265_RS14050 at 3535.073 kb on + strand, within CA265_RS14050 at 3535.090 kb on + strand, within CA265_RS14050 at 3535.145 kb on - strand, within CA265_RS14050 at 3535.234 kb on + strand, within CA265_RS14050 at 3535.331 kb on + strand, within CA265_RS14050 at 3535.332 kb on - strand, within CA265_RS14050 at 3535.435 kb on + strand, within CA265_RS14050 at 3535.548 kb on - strand at 3535.548 kb on - strand at 3535.550 kb on - strand at 3535.577 kb on + strand at 3535.577 kb on + strand at 3535.578 kb on - strand at 3535.612 kb on - strand at 3535.641 kb on - strand at 3535.646 kb on - strand at 3536.092 kb on + strand at 3536.094 kb on + strand at 3536.095 kb on - strand at 3536.095 kb on - strand at 3536.095 kb on - strand at 3536.099 kb on - strand at 3536.099 kb on - strand at 3536.102 kb on + strand at 3536.103 kb on - strand at 3536.180 kb on + strand at 3536.180 kb on + strand at 3536.267 kb on + strand at 3536.342 kb on + strand at 3536.343 kb on - strand at 3536.370 kb on + strand at 3536.506 kb on + strand, within CA265_RS14055 at 3536.511 kb on + strand, within CA265_RS14055 at 3536.511 kb on + strand, within CA265_RS14055 at 3536.512 kb on - strand, within CA265_RS14055 at 3536.652 kb on + strand, within CA265_RS14055 at 3536.653 kb on - strand, within CA265_RS14055 at 3536.653 kb on - strand, within CA265_RS14055
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 3,533,232 + CA265_RS14045 0.22 -0.2 3,533,311 + CA265_RS14045 0.30 -0.5 3,533,312 - CA265_RS14045 0.30 -0.5 3,533,381 - CA265_RS14045 0.38 +2.7 3,533,408 + CA265_RS14045 0.41 -0.6 3,533,409 - CA265_RS14045 0.41 +0.1 3,533,438 - CA265_RS14045 0.44 -0.9 3,533,447 + CA265_RS14045 0.45 -0.5 3,533,456 + CA265_RS14045 0.46 -1.1 3,533,457 - CA265_RS14045 0.46 +0.2 3,533,476 + CA265_RS14045 0.48 +1.1 3,533,531 - CA265_RS14045 0.54 -0.5 3,533,643 + CA265_RS14045 0.66 -0.8 3,533,693 + CA265_RS14045 0.71 +0.0 3,533,693 + CA265_RS14045 0.71 +0.6 3,533,694 - CA265_RS14045 0.72 -0.5 3,533,694 - CA265_RS14045 0.72 -0.4 3,533,697 + CA265_RS14045 0.72 -1.2 3,533,697 + CA265_RS14045 0.72 +0.3 3,533,711 + CA265_RS14045 0.73 +0.0 3,533,726 - CA265_RS14045 0.75 -0.2 3,533,763 + CA265_RS14045 0.79 +0.0 3,533,764 - CA265_RS14045 0.79 +0.6 3,533,822 + CA265_RS14045 0.85 +0.3 3,533,858 + CA265_RS14045 0.89 +0.7 3,533,859 - CA265_RS14045 0.89 -0.1 3,533,859 - CA265_RS14045 0.89 -1.2 3,533,914 + +0.7 3,533,915 - +0.7 3,533,915 - -0.2 3,533,915 - -0.6 3,533,945 + -0.6 3,534,049 - -0.6 3,534,086 + -0.4 3,534,124 - +2.1 3,534,181 - +0.1 3,534,223 - +0.8 3,534,288 - -0.4 3,534,434 + CA265_RS14050 0.16 +0.4 3,534,435 - CA265_RS14050 0.16 +0.4 3,534,435 - CA265_RS14050 0.16 +1.1 3,534,488 + CA265_RS14050 0.20 -0.8 3,534,609 + CA265_RS14050 0.28 -0.6 3,534,610 - CA265_RS14050 0.28 -0.5 3,534,626 + CA265_RS14050 0.29 -0.5 3,534,626 + CA265_RS14050 0.29 +0.2 3,534,631 + CA265_RS14050 0.29 +0.2 3,534,699 - CA265_RS14050 0.34 -0.5 3,534,800 + CA265_RS14050 0.41 +0.5 3,534,903 - CA265_RS14050 0.48 -0.1 3,534,948 + CA265_RS14050 0.51 -1.1 3,534,975 - CA265_RS14050 0.52 -0.2 3,535,073 + CA265_RS14050 0.59 -0.6 3,535,090 + CA265_RS14050 0.60 -0.7 3,535,145 - CA265_RS14050 0.64 +0.5 3,535,234 + CA265_RS14050 0.70 -0.4 3,535,331 + CA265_RS14050 0.76 +0.1 3,535,332 - CA265_RS14050 0.76 +0.8 3,535,435 + CA265_RS14050 0.83 -0.6 3,535,548 - -0.2 3,535,548 - +0.5 3,535,550 - -0.1 3,535,577 + -0.6 3,535,577 + -0.3 3,535,578 - -1.7 3,535,612 - -0.7 3,535,641 - +0.2 3,535,646 - +1.0 3,536,092 + +0.8 3,536,094 + -1.2 3,536,095 - +0.5 3,536,095 - -0.9 3,536,095 - -0.8 3,536,099 - +0.9 3,536,099 - -0.1 3,536,102 + +0.2 3,536,103 - -0.6 3,536,180 + +0.8 3,536,180 + -0.5 3,536,267 + -0.8 3,536,342 + -0.7 3,536,343 - +0.3 3,536,370 + -0.8 3,536,506 + CA265_RS14055 0.14 -0.2 3,536,511 + CA265_RS14055 0.15 -0.1 3,536,511 + CA265_RS14055 0.15 +0.5 3,536,512 - CA265_RS14055 0.15 +0.1 3,536,652 + CA265_RS14055 0.42 -0.4 3,536,653 - CA265_RS14055 0.42 -1.9 3,536,653 - CA265_RS14055 0.42 -0.8
Or see this region's nucleotide sequence