Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS11120 and CA265_RS11125 are separated by 373 nucleotides CA265_RS11125 and CA265_RS11130 are separated by 20 nucleotides CA265_RS11130 and CA265_RS11135 are separated by 76 nucleotides
CA265_RS11120: CA265_RS11120 - phosphohydrolase, at 2,736,459 to 2,737,673
_RS11120
CA265_RS11125: CA265_RS11125 - hypothetical protein, at 2,738,047 to 2,738,310
_RS11125
CA265_RS11130: CA265_RS11130 - alpha-galactosidase, at 2,738,331 to 2,740,517
_RS11130
CA265_RS11135: CA265_RS11135 - heparan N-sulfatase, at 2,740,594 to 2,742,216
_RS11135
Position (kb)
2738
2739
2740
2741 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2737.359 kb on + strand, within CA265_RS11120 at 2737.359 kb on + strand, within CA265_RS11120 at 2737.370 kb on + strand, within CA265_RS11120 at 2737.371 kb on - strand, within CA265_RS11120 at 2737.452 kb on + strand, within CA265_RS11120 at 2737.452 kb on + strand, within CA265_RS11120 at 2737.469 kb on - strand, within CA265_RS11120 at 2737.469 kb on - strand, within CA265_RS11120 at 2737.487 kb on - strand, within CA265_RS11120 at 2737.574 kb on + strand at 2737.575 kb on - strand at 2737.587 kb on - strand at 2737.587 kb on - strand at 2737.624 kb on + strand at 2737.667 kb on - strand at 2737.699 kb on + strand at 2737.699 kb on + strand at 2737.702 kb on + strand at 2737.832 kb on + strand at 2737.843 kb on - strand at 2737.877 kb on + strand at 2737.878 kb on - strand at 2737.902 kb on - strand at 2737.933 kb on + strand at 2737.990 kb on + strand at 2737.991 kb on - strand at 2737.993 kb on - strand at 2738.090 kb on + strand, within CA265_RS11125 at 2738.120 kb on - strand, within CA265_RS11125 at 2738.120 kb on - strand, within CA265_RS11125 at 2738.159 kb on - strand, within CA265_RS11125 at 2738.203 kb on + strand, within CA265_RS11125 at 2738.209 kb on + strand, within CA265_RS11125 at 2738.214 kb on - strand, within CA265_RS11125 at 2738.228 kb on + strand, within CA265_RS11125 at 2738.308 kb on + strand at 2738.309 kb on - strand at 2738.374 kb on + strand at 2738.374 kb on + strand at 2738.375 kb on - strand at 2738.375 kb on - strand at 2738.428 kb on + strand at 2738.429 kb on - strand at 2738.429 kb on - strand at 2738.472 kb on + strand at 2738.472 kb on + strand at 2738.516 kb on - strand at 2738.528 kb on + strand at 2738.529 kb on - strand at 2738.529 kb on - strand at 2738.529 kb on - strand at 2738.532 kb on + strand at 2738.557 kb on + strand, within CA265_RS11130 at 2738.574 kb on + strand, within CA265_RS11130 at 2738.574 kb on + strand, within CA265_RS11130 at 2738.574 kb on + strand, within CA265_RS11130 at 2738.725 kb on + strand, within CA265_RS11130 at 2738.730 kb on + strand, within CA265_RS11130 at 2738.745 kb on - strand, within CA265_RS11130 at 2738.808 kb on - strand, within CA265_RS11130 at 2738.894 kb on + strand, within CA265_RS11130 at 2738.895 kb on - strand, within CA265_RS11130 at 2738.939 kb on - strand, within CA265_RS11130 at 2738.945 kb on + strand, within CA265_RS11130 at 2738.948 kb on - strand, within CA265_RS11130 at 2739.016 kb on + strand, within CA265_RS11130 at 2739.207 kb on + strand, within CA265_RS11130 at 2739.208 kb on - strand, within CA265_RS11130 at 2739.208 kb on - strand, within CA265_RS11130 at 2739.208 kb on - strand, within CA265_RS11130 at 2739.265 kb on - strand, within CA265_RS11130 at 2739.275 kb on - strand, within CA265_RS11130 at 2739.369 kb on - strand, within CA265_RS11130 at 2739.427 kb on - strand, within CA265_RS11130 at 2739.427 kb on - strand, within CA265_RS11130 at 2739.469 kb on - strand, within CA265_RS11130 at 2739.470 kb on + strand, within CA265_RS11130 at 2739.471 kb on - strand, within CA265_RS11130 at 2739.500 kb on + strand, within CA265_RS11130 at 2739.609 kb on - strand, within CA265_RS11130 at 2739.634 kb on - strand, within CA265_RS11130 at 2739.636 kb on + strand, within CA265_RS11130 at 2739.637 kb on - strand, within CA265_RS11130 at 2739.639 kb on - strand, within CA265_RS11130 at 2739.639 kb on - strand, within CA265_RS11130 at 2739.740 kb on + strand, within CA265_RS11130 at 2739.768 kb on + strand, within CA265_RS11130 at 2739.769 kb on - strand, within CA265_RS11130 at 2739.769 kb on - strand, within CA265_RS11130 at 2739.769 kb on - strand, within CA265_RS11130 at 2739.786 kb on - strand, within CA265_RS11130 at 2739.810 kb on + strand, within CA265_RS11130 at 2739.819 kb on + strand, within CA265_RS11130 at 2739.820 kb on - strand, within CA265_RS11130 at 2739.874 kb on + strand, within CA265_RS11130 at 2739.911 kb on - strand, within CA265_RS11130 at 2739.915 kb on + strand, within CA265_RS11130 at 2739.916 kb on - strand, within CA265_RS11130 at 2739.916 kb on - strand, within CA265_RS11130 at 2739.996 kb on + strand, within CA265_RS11130 at 2740.003 kb on - strand, within CA265_RS11130 at 2740.049 kb on + strand, within CA265_RS11130 at 2740.061 kb on + strand, within CA265_RS11130 at 2740.102 kb on - strand, within CA265_RS11130 at 2740.115 kb on - strand, within CA265_RS11130 at 2740.168 kb on - strand, within CA265_RS11130 at 2740.173 kb on - strand, within CA265_RS11130 at 2740.173 kb on - strand, within CA265_RS11130 at 2740.173 kb on - strand, within CA265_RS11130 at 2740.184 kb on + strand, within CA265_RS11130 at 2740.188 kb on - strand, within CA265_RS11130 at 2740.225 kb on - strand, within CA265_RS11130 at 2740.287 kb on - strand, within CA265_RS11130 at 2740.311 kb on - strand at 2740.339 kb on + strand at 2740.349 kb on - strand at 2740.382 kb on + strand at 2740.416 kb on - strand at 2740.418 kb on + strand at 2740.419 kb on - strand at 2740.421 kb on + strand at 2740.453 kb on + strand at 2740.460 kb on + strand at 2740.504 kb on + strand at 2740.505 kb on - strand at 2740.545 kb on - strand at 2740.574 kb on - strand at 2740.576 kb on - strand at 2740.583 kb on + strand at 2740.585 kb on + strand at 2740.586 kb on - strand at 2740.586 kb on - strand at 2740.586 kb on - strand at 2740.719 kb on + strand at 2740.719 kb on + strand at 2740.742 kb on - strand at 2740.750 kb on - strand at 2740.816 kb on - strand, within CA265_RS11135 at 2740.816 kb on - strand, within CA265_RS11135 at 2740.851 kb on + strand, within CA265_RS11135 at 2740.851 kb on + strand, within CA265_RS11135 at 2740.852 kb on - strand, within CA265_RS11135 at 2740.970 kb on - strand, within CA265_RS11135 at 2740.972 kb on - strand, within CA265_RS11135 at 2741.016 kb on + strand, within CA265_RS11135 at 2741.017 kb on - strand, within CA265_RS11135 at 2741.091 kb on - strand, within CA265_RS11135 at 2741.096 kb on - strand, within CA265_RS11135 at 2741.201 kb on + strand, within CA265_RS11135 at 2741.296 kb on + strand, within CA265_RS11135 at 2741.314 kb on + strand, within CA265_RS11135 at 2741.315 kb on - strand, within CA265_RS11135 at 2741.381 kb on + strand, within CA265_RS11135 at 2741.381 kb on + strand at 2741.381 kb on + strand, within CA265_RS11135 at 2741.381 kb on + strand, within CA265_RS11135 at 2741.400 kb on + strand, within CA265_RS11135 at 2741.444 kb on - strand, within CA265_RS11135 at 2741.493 kb on + strand, within CA265_RS11135
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 2,737,359 + CA265_RS11120 0.74 +0.3 2,737,359 + CA265_RS11120 0.74 -0.5 2,737,370 + CA265_RS11120 0.75 -0.7 2,737,371 - CA265_RS11120 0.75 +1.3 2,737,452 + CA265_RS11120 0.82 -0.2 2,737,452 + CA265_RS11120 0.82 +0.8 2,737,469 - CA265_RS11120 0.83 +0.2 2,737,469 - CA265_RS11120 0.83 +1.8 2,737,487 - CA265_RS11120 0.85 +2.2 2,737,574 + -1.3 2,737,575 - +0.1 2,737,587 - +0.8 2,737,587 - +1.6 2,737,624 + -1.7 2,737,667 - -0.7 2,737,699 + +0.8 2,737,699 + -1.1 2,737,702 + -2.1 2,737,832 + -0.3 2,737,843 - -0.0 2,737,877 + -0.5 2,737,878 - -1.0 2,737,902 - -1.0 2,737,933 + -0.9 2,737,990 + -0.0 2,737,991 - +0.5 2,737,993 - -0.1 2,738,090 + CA265_RS11125 0.16 +0.1 2,738,120 - CA265_RS11125 0.28 +0.1 2,738,120 - CA265_RS11125 0.28 +0.5 2,738,159 - CA265_RS11125 0.42 +1.0 2,738,203 + CA265_RS11125 0.59 -0.8 2,738,209 + CA265_RS11125 0.61 -0.8 2,738,214 - CA265_RS11125 0.63 +0.6 2,738,228 + CA265_RS11125 0.69 -0.3 2,738,308 + -0.6 2,738,309 - -0.3 2,738,374 + -2.4 2,738,374 + +0.2 2,738,375 - -0.1 2,738,375 - +0.2 2,738,428 + +1.9 2,738,429 - +0.5 2,738,429 - +0.4 2,738,472 + +0.6 2,738,472 + +0.3 2,738,516 - +0.1 2,738,528 + -0.7 2,738,529 - +0.0 2,738,529 - +0.6 2,738,529 - -0.7 2,738,532 + -0.3 2,738,557 + CA265_RS11130 0.10 -0.7 2,738,574 + CA265_RS11130 0.11 -0.2 2,738,574 + CA265_RS11130 0.11 +0.0 2,738,574 + CA265_RS11130 0.11 +0.4 2,738,725 + CA265_RS11130 0.18 -0.1 2,738,730 + CA265_RS11130 0.18 -0.4 2,738,745 - CA265_RS11130 0.19 -0.4 2,738,808 - CA265_RS11130 0.22 -0.2 2,738,894 + CA265_RS11130 0.26 +0.2 2,738,895 - CA265_RS11130 0.26 +0.8 2,738,939 - CA265_RS11130 0.28 -0.9 2,738,945 + CA265_RS11130 0.28 -1.0 2,738,948 - CA265_RS11130 0.28 -0.3 2,739,016 + CA265_RS11130 0.31 +0.4 2,739,207 + CA265_RS11130 0.40 +0.9 2,739,208 - CA265_RS11130 0.40 -1.6 2,739,208 - CA265_RS11130 0.40 +3.9 2,739,208 - CA265_RS11130 0.40 -1.4 2,739,265 - CA265_RS11130 0.43 -1.1 2,739,275 - CA265_RS11130 0.43 -0.6 2,739,369 - CA265_RS11130 0.47 -0.0 2,739,427 - CA265_RS11130 0.50 -1.1 2,739,427 - CA265_RS11130 0.50 -1.2 2,739,469 - CA265_RS11130 0.52 -0.4 2,739,470 + CA265_RS11130 0.52 -0.6 2,739,471 - CA265_RS11130 0.52 -0.2 2,739,500 + CA265_RS11130 0.53 +0.6 2,739,609 - CA265_RS11130 0.58 -0.1 2,739,634 - CA265_RS11130 0.60 -0.1 2,739,636 + CA265_RS11130 0.60 -0.7 2,739,637 - CA265_RS11130 0.60 +0.4 2,739,639 - CA265_RS11130 0.60 -0.4 2,739,639 - CA265_RS11130 0.60 +0.1 2,739,740 + CA265_RS11130 0.64 -1.0 2,739,768 + CA265_RS11130 0.66 -1.5 2,739,769 - CA265_RS11130 0.66 -2.1 2,739,769 - CA265_RS11130 0.66 -0.1 2,739,769 - CA265_RS11130 0.66 -0.0 2,739,786 - CA265_RS11130 0.67 +0.8 2,739,810 + CA265_RS11130 0.68 -0.6 2,739,819 + CA265_RS11130 0.68 +0.1 2,739,820 - CA265_RS11130 0.68 -0.1 2,739,874 + CA265_RS11130 0.71 -0.5 2,739,911 - CA265_RS11130 0.72 -0.3 2,739,915 + CA265_RS11130 0.72 -0.0 2,739,916 - CA265_RS11130 0.72 -0.6 2,739,916 - CA265_RS11130 0.72 +0.4 2,739,996 + CA265_RS11130 0.76 +0.5 2,740,003 - CA265_RS11130 0.76 -0.4 2,740,049 + CA265_RS11130 0.79 -0.6 2,740,061 + CA265_RS11130 0.79 +0.8 2,740,102 - CA265_RS11130 0.81 -1.0 2,740,115 - CA265_RS11130 0.82 +0.1 2,740,168 - CA265_RS11130 0.84 +0.0 2,740,173 - CA265_RS11130 0.84 -0.1 2,740,173 - CA265_RS11130 0.84 -0.1 2,740,173 - CA265_RS11130 0.84 +0.2 2,740,184 + CA265_RS11130 0.85 -0.5 2,740,188 - CA265_RS11130 0.85 +0.5 2,740,225 - CA265_RS11130 0.87 +0.3 2,740,287 - CA265_RS11130 0.89 +0.7 2,740,311 - +0.2 2,740,339 + -0.8 2,740,349 - +0.1 2,740,382 + -0.3 2,740,416 - -0.3 2,740,418 + -0.8 2,740,419 - +1.0 2,740,421 + -0.3 2,740,453 + +0.1 2,740,460 + +0.6 2,740,504 + -1.0 2,740,505 - -0.8 2,740,545 - +0.2 2,740,574 - -0.8 2,740,576 - +0.6 2,740,583 + -0.1 2,740,585 + -0.0 2,740,586 - -0.3 2,740,586 - -1.3 2,740,586 - +0.9 2,740,719 + +1.2 2,740,719 + -0.5 2,740,742 - -0.2 2,740,750 - -0.8 2,740,816 - CA265_RS11135 0.14 -1.0 2,740,816 - CA265_RS11135 0.14 +0.1 2,740,851 + CA265_RS11135 0.16 -0.1 2,740,851 + CA265_RS11135 0.16 +0.8 2,740,852 - CA265_RS11135 0.16 -0.2 2,740,970 - CA265_RS11135 0.23 -0.3 2,740,972 - CA265_RS11135 0.23 +0.4 2,741,016 + CA265_RS11135 0.26 +0.4 2,741,017 - CA265_RS11135 0.26 -0.4 2,741,091 - CA265_RS11135 0.31 +0.1 2,741,096 - CA265_RS11135 0.31 +1.1 2,741,201 + CA265_RS11135 0.37 +0.9 2,741,296 + CA265_RS11135 0.43 +0.4 2,741,314 + CA265_RS11135 0.44 -0.6 2,741,315 - CA265_RS11135 0.44 +0.6 2,741,381 + CA265_RS11135 0.48 +1.4 2,741,381 + +0.4 2,741,381 + CA265_RS11135 0.48 +0.5 2,741,381 + CA265_RS11135 0.48 +0.6 2,741,400 + CA265_RS11135 0.50 +1.8 2,741,444 - CA265_RS11135 0.52 -1.4 2,741,493 + CA265_RS11135 0.55 -0.2
Or see this region's nucleotide sequence