Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS10675 and CA265_RS10680 are separated by 218 nucleotides CA265_RS10680 and CA265_RS10685 are separated by 143 nucleotides
CA265_RS10675: CA265_RS10675 - IMP dehydrogenase, at 2,638,807 to 2,640,276
_RS10675
CA265_RS10680: CA265_RS10680 - fatty acid hydroxylase, at 2,640,495 to 2,641,502
_RS10680
CA265_RS10685: CA265_RS10685 - transglutaminase, at 2,641,646 to 2,643,652
_RS10685
Position (kb)
2640
2641
2642 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2640.464 kb on + strand at 2640.467 kb on - strand at 2640.487 kb on - strand at 2640.489 kb on - strand at 2640.490 kb on + strand at 2640.582 kb on + strand at 2640.583 kb on - strand at 2640.584 kb on + strand at 2640.594 kb on - strand at 2640.659 kb on + strand, within CA265_RS10680 at 2640.695 kb on - strand, within CA265_RS10680 at 2640.795 kb on + strand, within CA265_RS10680 at 2640.837 kb on + strand, within CA265_RS10680 at 2640.837 kb on + strand, within CA265_RS10680 at 2640.837 kb on + strand, within CA265_RS10680 at 2640.838 kb on - strand, within CA265_RS10680 at 2640.847 kb on + strand, within CA265_RS10680 at 2640.847 kb on + strand, within CA265_RS10680 at 2640.847 kb on + strand, within CA265_RS10680 at 2640.847 kb on + strand, within CA265_RS10680 at 2640.847 kb on + strand, within CA265_RS10680 at 2640.847 kb on + strand, within CA265_RS10680 at 2640.847 kb on + strand, within CA265_RS10680 at 2640.848 kb on - strand, within CA265_RS10680 at 2640.874 kb on - strand, within CA265_RS10680 at 2640.949 kb on + strand, within CA265_RS10680 at 2641.030 kb on + strand, within CA265_RS10680 at 2641.031 kb on - strand, within CA265_RS10680 at 2641.031 kb on - strand, within CA265_RS10680 at 2641.061 kb on + strand, within CA265_RS10680 at 2641.137 kb on - strand, within CA265_RS10680 at 2641.191 kb on + strand, within CA265_RS10680 at 2641.191 kb on + strand, within CA265_RS10680 at 2641.199 kb on + strand, within CA265_RS10680 at 2641.242 kb on - strand, within CA265_RS10680 at 2641.248 kb on + strand, within CA265_RS10680 at 2641.319 kb on - strand, within CA265_RS10680 at 2641.326 kb on + strand, within CA265_RS10680 at 2641.326 kb on + strand, within CA265_RS10680 at 2641.326 kb on + strand, within CA265_RS10680 at 2641.327 kb on - strand, within CA265_RS10680 at 2641.543 kb on - strand at 2641.596 kb on - strand at 2641.642 kb on - strand at 2641.678 kb on - strand at 2641.696 kb on + strand at 2641.697 kb on - strand at 2641.697 kb on - strand at 2641.717 kb on + strand at 2641.767 kb on - strand at 2641.838 kb on + strand at 2641.839 kb on - strand at 2641.839 kb on - strand at 2641.871 kb on - strand, within CA265_RS10685 at 2641.960 kb on + strand, within CA265_RS10685 at 2642.007 kb on - strand, within CA265_RS10685 at 2642.085 kb on - strand, within CA265_RS10685 at 2642.085 kb on - strand, within CA265_RS10685 at 2642.110 kb on + strand, within CA265_RS10685 at 2642.128 kb on + strand, within CA265_RS10685 at 2642.165 kb on - strand, within CA265_RS10685 at 2642.229 kb on + strand, within CA265_RS10685 at 2642.230 kb on - strand, within CA265_RS10685 at 2642.244 kb on - strand, within CA265_RS10685 at 2642.276 kb on + strand, within CA265_RS10685 at 2642.276 kb on + strand, within CA265_RS10685 at 2642.307 kb on + strand, within CA265_RS10685 at 2642.318 kb on + strand, within CA265_RS10685 at 2642.319 kb on - strand, within CA265_RS10685 at 2642.354 kb on - strand, within CA265_RS10685 at 2642.357 kb on + strand, within CA265_RS10685 at 2642.358 kb on - strand, within CA265_RS10685 at 2642.420 kb on - strand, within CA265_RS10685 at 2642.420 kb on - strand, within CA265_RS10685
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 2,640,464 + +1.4 2,640,467 - -1.0 2,640,487 - -2.9 2,640,489 - -1.3 2,640,490 + -0.1 2,640,582 + -0.5 2,640,583 - -1.1 2,640,584 + +0.1 2,640,594 - -0.6 2,640,659 + CA265_RS10680 0.16 +0.8 2,640,695 - CA265_RS10680 0.20 +0.2 2,640,795 + CA265_RS10680 0.30 +0.3 2,640,837 + CA265_RS10680 0.34 -0.1 2,640,837 + CA265_RS10680 0.34 +0.8 2,640,837 + CA265_RS10680 0.34 -0.0 2,640,838 - CA265_RS10680 0.34 -1.0 2,640,847 + CA265_RS10680 0.35 +1.9 2,640,847 + CA265_RS10680 0.35 -0.6 2,640,847 + CA265_RS10680 0.35 +0.2 2,640,847 + CA265_RS10680 0.35 +1.1 2,640,847 + CA265_RS10680 0.35 +0.0 2,640,847 + CA265_RS10680 0.35 +0.5 2,640,847 + CA265_RS10680 0.35 +0.0 2,640,848 - CA265_RS10680 0.35 -1.6 2,640,874 - CA265_RS10680 0.38 +0.4 2,640,949 + CA265_RS10680 0.45 -1.3 2,641,030 + CA265_RS10680 0.53 +0.2 2,641,031 - CA265_RS10680 0.53 -0.1 2,641,031 - CA265_RS10680 0.53 +3.4 2,641,061 + CA265_RS10680 0.56 -0.2 2,641,137 - CA265_RS10680 0.64 +1.9 2,641,191 + CA265_RS10680 0.69 -0.1 2,641,191 + CA265_RS10680 0.69 -1.1 2,641,199 + CA265_RS10680 0.70 +0.4 2,641,242 - CA265_RS10680 0.74 +1.4 2,641,248 + CA265_RS10680 0.75 -0.3 2,641,319 - CA265_RS10680 0.82 -0.4 2,641,326 + CA265_RS10680 0.82 +0.0 2,641,326 + CA265_RS10680 0.82 -0.6 2,641,326 + CA265_RS10680 0.82 +0.2 2,641,327 - CA265_RS10680 0.83 -0.8 2,641,543 - -0.3 2,641,596 - -0.5 2,641,642 - +0.9 2,641,678 - +0.3 2,641,696 + -1.0 2,641,697 - -0.8 2,641,697 - -0.0 2,641,717 + -1.3 2,641,767 - +0.1 2,641,838 + -0.3 2,641,839 - +0.8 2,641,839 - +0.6 2,641,871 - CA265_RS10685 0.11 -0.5 2,641,960 + CA265_RS10685 0.16 -2.3 2,642,007 - CA265_RS10685 0.18 +0.1 2,642,085 - CA265_RS10685 0.22 -0.4 2,642,085 - CA265_RS10685 0.22 -0.5 2,642,110 + CA265_RS10685 0.23 -0.1 2,642,128 + CA265_RS10685 0.24 -0.4 2,642,165 - CA265_RS10685 0.26 +0.7 2,642,229 + CA265_RS10685 0.29 -1.1 2,642,230 - CA265_RS10685 0.29 -1.0 2,642,244 - CA265_RS10685 0.30 +0.4 2,642,276 + CA265_RS10685 0.31 -0.1 2,642,276 + CA265_RS10685 0.31 +1.7 2,642,307 + CA265_RS10685 0.33 +0.6 2,642,318 + CA265_RS10685 0.33 +1.4 2,642,319 - CA265_RS10685 0.34 +2.0 2,642,354 - CA265_RS10685 0.35 -0.5 2,642,357 + CA265_RS10685 0.35 -0.1 2,642,358 - CA265_RS10685 0.35 +0.2 2,642,420 - CA265_RS10685 0.39 -1.0 2,642,420 - CA265_RS10685 0.39 +1.1
Or see this region's nucleotide sequence