Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS10420 and CA265_RS10425 are separated by 56 nucleotides CA265_RS10425 and CA265_RS10430 are separated by 205 nucleotides CA265_RS10430 and CA265_RS10435 are separated by 17 nucleotides CA265_RS10435 and CA265_RS10440 are separated by 114 nucleotides
CA265_RS10420: CA265_RS10420 - hydroxyacid dehydrogenase, at 2,577,972 to 2,578,976
_RS10420
CA265_RS10425: CA265_RS10425 - hypothetical protein, at 2,579,033 to 2,581,792
_RS10425
CA265_RS10430: CA265_RS10430 - hypothetical protein, at 2,581,998 to 2,582,252
_RS10430
CA265_RS10435: CA265_RS10435 - RNA-binding protein, at 2,582,270 to 2,582,461
_RS10435
CA265_RS10440: CA265_RS10440 - hypothetical protein, at 2,582,576 to 2,585,344
_RS10440
Position (kb)
2579
2580
2581
2582 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2578.133 kb on + strand, within CA265_RS10420 at 2578.152 kb on - strand, within CA265_RS10420 at 2578.260 kb on - strand, within CA265_RS10420 at 2578.287 kb on + strand, within CA265_RS10420 at 2578.288 kb on - strand, within CA265_RS10420 at 2578.288 kb on - strand, within CA265_RS10420 at 2578.352 kb on + strand, within CA265_RS10420 at 2578.411 kb on + strand, within CA265_RS10420 at 2578.411 kb on + strand, within CA265_RS10420 at 2578.412 kb on - strand, within CA265_RS10420 at 2578.602 kb on - strand, within CA265_RS10420 at 2578.603 kb on + strand, within CA265_RS10420 at 2578.631 kb on + strand, within CA265_RS10420 at 2578.733 kb on + strand, within CA265_RS10420 at 2578.787 kb on + strand, within CA265_RS10420 at 2578.788 kb on - strand, within CA265_RS10420 at 2578.806 kb on - strand, within CA265_RS10420 at 2578.811 kb on + strand, within CA265_RS10420 at 2578.818 kb on + strand, within CA265_RS10420 at 2578.909 kb on + strand at 2578.966 kb on - strand at 2578.977 kb on - strand at 2578.995 kb on + strand at 2578.999 kb on + strand at 2579.060 kb on + strand at 2579.061 kb on - strand at 2579.153 kb on + strand at 2579.162 kb on + strand at 2579.163 kb on - strand at 2579.198 kb on + strand at 2579.198 kb on + strand at 2579.206 kb on + strand at 2579.213 kb on + strand at 2579.253 kb on + strand at 2579.254 kb on - strand at 2579.254 kb on - strand at 2579.274 kb on + strand at 2579.275 kb on - strand at 2579.332 kb on - strand, within CA265_RS10425 at 2579.382 kb on + strand, within CA265_RS10425 at 2579.382 kb on + strand, within CA265_RS10425 at 2579.418 kb on - strand, within CA265_RS10425 at 2579.491 kb on + strand, within CA265_RS10425 at 2579.623 kb on - strand, within CA265_RS10425 at 2579.664 kb on + strand, within CA265_RS10425 at 2579.669 kb on + strand, within CA265_RS10425 at 2579.669 kb on + strand, within CA265_RS10425 at 2579.705 kb on - strand, within CA265_RS10425 at 2579.705 kb on - strand, within CA265_RS10425 at 2579.710 kb on + strand, within CA265_RS10425 at 2579.760 kb on + strand, within CA265_RS10425 at 2579.767 kb on + strand, within CA265_RS10425 at 2579.828 kb on + strand, within CA265_RS10425 at 2579.845 kb on + strand, within CA265_RS10425 at 2579.855 kb on + strand, within CA265_RS10425 at 2579.871 kb on - strand, within CA265_RS10425 at 2579.891 kb on + strand, within CA265_RS10425 at 2579.891 kb on + strand, within CA265_RS10425 at 2579.892 kb on - strand, within CA265_RS10425 at 2579.921 kb on + strand, within CA265_RS10425 at 2579.927 kb on + strand, within CA265_RS10425 at 2579.928 kb on - strand, within CA265_RS10425 at 2579.928 kb on - strand, within CA265_RS10425 at 2579.998 kb on + strand, within CA265_RS10425 at 2580.064 kb on + strand, within CA265_RS10425 at 2580.069 kb on - strand, within CA265_RS10425 at 2580.195 kb on - strand, within CA265_RS10425 at 2580.195 kb on - strand, within CA265_RS10425 at 2580.195 kb on - strand, within CA265_RS10425 at 2580.271 kb on - strand, within CA265_RS10425 at 2580.271 kb on - strand, within CA265_RS10425 at 2580.413 kb on - strand, within CA265_RS10425 at 2580.464 kb on - strand, within CA265_RS10425 at 2580.465 kb on + strand, within CA265_RS10425 at 2580.465 kb on + strand, within CA265_RS10425 at 2580.465 kb on + strand, within CA265_RS10425 at 2580.465 kb on + strand, within CA265_RS10425 at 2580.465 kb on + strand, within CA265_RS10425 at 2580.465 kb on + strand, within CA265_RS10425 at 2580.466 kb on - strand, within CA265_RS10425 at 2580.466 kb on - strand, within CA265_RS10425 at 2580.529 kb on + strand, within CA265_RS10425 at 2580.546 kb on + strand, within CA265_RS10425 at 2580.547 kb on - strand, within CA265_RS10425 at 2580.556 kb on - strand, within CA265_RS10425 at 2580.577 kb on + strand, within CA265_RS10425 at 2580.609 kb on - strand, within CA265_RS10425 at 2580.610 kb on + strand, within CA265_RS10425 at 2580.614 kb on - strand, within CA265_RS10425 at 2580.627 kb on - strand, within CA265_RS10425 at 2580.627 kb on - strand, within CA265_RS10425 at 2580.714 kb on + strand, within CA265_RS10425 at 2580.715 kb on - strand, within CA265_RS10425 at 2580.716 kb on + strand, within CA265_RS10425 at 2580.717 kb on - strand at 2580.836 kb on + strand, within CA265_RS10425 at 2580.841 kb on + strand, within CA265_RS10425 at 2580.841 kb on + strand, within CA265_RS10425 at 2580.842 kb on - strand, within CA265_RS10425 at 2580.904 kb on + strand, within CA265_RS10425 at 2580.973 kb on + strand, within CA265_RS10425 at 2580.974 kb on - strand, within CA265_RS10425 at 2581.038 kb on - strand, within CA265_RS10425 at 2581.038 kb on - strand, within CA265_RS10425 at 2581.041 kb on - strand, within CA265_RS10425 at 2581.073 kb on + strand, within CA265_RS10425 at 2581.073 kb on + strand, within CA265_RS10425 at 2581.074 kb on - strand, within CA265_RS10425 at 2581.330 kb on + strand, within CA265_RS10425 at 2581.331 kb on - strand, within CA265_RS10425 at 2581.331 kb on - strand, within CA265_RS10425 at 2581.345 kb on + strand, within CA265_RS10425 at 2581.356 kb on - strand, within CA265_RS10425 at 2581.397 kb on - strand, within CA265_RS10425 at 2581.463 kb on + strand, within CA265_RS10425 at 2581.463 kb on + strand, within CA265_RS10425 at 2581.522 kb on + strand at 2581.522 kb on + strand at 2581.546 kb on - strand at 2581.573 kb on + strand at 2581.607 kb on + strand at 2581.608 kb on - strand at 2581.632 kb on - strand at 2581.644 kb on - strand at 2581.666 kb on + strand at 2581.672 kb on - strand at 2581.770 kb on - strand at 2581.775 kb on + strand at 2581.798 kb on - strand at 2581.897 kb on + strand at 2581.997 kb on + strand at 2582.071 kb on + strand, within CA265_RS10430 at 2582.071 kb on + strand, within CA265_RS10430 at 2582.102 kb on + strand, within CA265_RS10430 at 2582.116 kb on + strand, within CA265_RS10430 at 2582.129 kb on + strand, within CA265_RS10430 at 2582.171 kb on + strand, within CA265_RS10430 at 2582.199 kb on - strand, within CA265_RS10430 at 2582.251 kb on - strand at 2582.355 kb on + strand, within CA265_RS10435 at 2582.355 kb on + strand, within CA265_RS10435 at 2582.356 kb on - strand, within CA265_RS10435 at 2582.366 kb on - strand, within CA265_RS10435 at 2582.380 kb on + strand, within CA265_RS10435 at 2582.381 kb on - strand, within CA265_RS10435 at 2582.387 kb on - strand, within CA265_RS10435 at 2582.410 kb on + strand, within CA265_RS10435 at 2582.440 kb on - strand, within CA265_RS10435 at 2582.442 kb on + strand at 2582.442 kb on + strand at 2582.460 kb on - strand at 2582.547 kb on + strand at 2582.564 kb on - strand at 2582.674 kb on + strand at 2582.675 kb on - strand at 2582.696 kb on - strand at 2582.716 kb on + strand at 2582.717 kb on - strand at 2582.779 kb on + strand at 2582.779 kb on + strand at 2582.792 kb on - strand at 2582.792 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 2,578,133 + CA265_RS10420 0.16 +0.5 2,578,152 - CA265_RS10420 0.18 +0.5 2,578,260 - CA265_RS10420 0.29 +0.3 2,578,287 + CA265_RS10420 0.31 -1.1 2,578,288 - CA265_RS10420 0.31 +0.1 2,578,288 - CA265_RS10420 0.31 -0.5 2,578,352 + CA265_RS10420 0.38 -1.5 2,578,411 + CA265_RS10420 0.44 -0.1 2,578,411 + CA265_RS10420 0.44 -0.1 2,578,412 - CA265_RS10420 0.44 -1.6 2,578,602 - CA265_RS10420 0.63 -0.4 2,578,603 + CA265_RS10420 0.63 -3.7 2,578,631 + CA265_RS10420 0.66 -0.6 2,578,733 + CA265_RS10420 0.76 -0.7 2,578,787 + CA265_RS10420 0.81 -0.7 2,578,788 - CA265_RS10420 0.81 +0.9 2,578,806 - CA265_RS10420 0.83 -0.0 2,578,811 + CA265_RS10420 0.83 -0.5 2,578,818 + CA265_RS10420 0.84 +0.6 2,578,909 + -0.5 2,578,966 - -0.8 2,578,977 - +0.8 2,578,995 + -0.3 2,578,999 + -0.9 2,579,060 + -0.3 2,579,061 - -0.0 2,579,153 + -0.9 2,579,162 + -1.2 2,579,163 - +1.2 2,579,198 + -0.2 2,579,198 + -0.6 2,579,206 + +0.2 2,579,213 + +0.9 2,579,253 + +0.7 2,579,254 - +0.4 2,579,254 - -0.3 2,579,274 + -0.4 2,579,275 - +0.6 2,579,332 - CA265_RS10425 0.11 +0.6 2,579,382 + CA265_RS10425 0.13 +0.5 2,579,382 + CA265_RS10425 0.13 -0.1 2,579,418 - CA265_RS10425 0.14 +0.5 2,579,491 + CA265_RS10425 0.17 -0.4 2,579,623 - CA265_RS10425 0.21 +0.2 2,579,664 + CA265_RS10425 0.23 -1.9 2,579,669 + CA265_RS10425 0.23 -0.4 2,579,669 + CA265_RS10425 0.23 -1.3 2,579,705 - CA265_RS10425 0.24 +1.3 2,579,705 - CA265_RS10425 0.24 +0.5 2,579,710 + CA265_RS10425 0.25 +0.3 2,579,760 + CA265_RS10425 0.26 +1.3 2,579,767 + CA265_RS10425 0.27 -0.0 2,579,828 + CA265_RS10425 0.29 -0.4 2,579,845 + CA265_RS10425 0.29 -2.8 2,579,855 + CA265_RS10425 0.30 +0.9 2,579,871 - CA265_RS10425 0.30 -0.6 2,579,891 + CA265_RS10425 0.31 -0.2 2,579,891 + CA265_RS10425 0.31 +0.0 2,579,892 - CA265_RS10425 0.31 +0.4 2,579,921 + CA265_RS10425 0.32 +1.1 2,579,927 + CA265_RS10425 0.32 -0.4 2,579,928 - CA265_RS10425 0.32 +1.1 2,579,928 - CA265_RS10425 0.32 +1.0 2,579,998 + CA265_RS10425 0.35 +0.5 2,580,064 + CA265_RS10425 0.37 +0.3 2,580,069 - CA265_RS10425 0.38 +0.6 2,580,195 - CA265_RS10425 0.42 -0.7 2,580,195 - CA265_RS10425 0.42 +0.3 2,580,195 - CA265_RS10425 0.42 -0.2 2,580,271 - CA265_RS10425 0.45 +0.7 2,580,271 - CA265_RS10425 0.45 +0.9 2,580,413 - CA265_RS10425 0.50 -2.7 2,580,464 - CA265_RS10425 0.52 +0.5 2,580,465 + CA265_RS10425 0.52 +0.3 2,580,465 + CA265_RS10425 0.52 +0.5 2,580,465 + CA265_RS10425 0.52 -0.9 2,580,465 + CA265_RS10425 0.52 +1.2 2,580,465 + CA265_RS10425 0.52 -2.8 2,580,465 + CA265_RS10425 0.52 -0.4 2,580,466 - CA265_RS10425 0.52 +1.9 2,580,466 - CA265_RS10425 0.52 -0.5 2,580,529 + CA265_RS10425 0.54 -1.0 2,580,546 + CA265_RS10425 0.55 -0.3 2,580,547 - CA265_RS10425 0.55 -0.1 2,580,556 - CA265_RS10425 0.55 +0.3 2,580,577 + CA265_RS10425 0.56 -1.0 2,580,609 - CA265_RS10425 0.57 +0.7 2,580,610 + CA265_RS10425 0.57 +0.8 2,580,614 - CA265_RS10425 0.57 +0.3 2,580,627 - CA265_RS10425 0.58 -0.9 2,580,627 - CA265_RS10425 0.58 +0.4 2,580,714 + CA265_RS10425 0.61 -0.4 2,580,715 - CA265_RS10425 0.61 -0.0 2,580,716 + CA265_RS10425 0.61 -1.4 2,580,717 - +0.3 2,580,836 + CA265_RS10425 0.65 +0.5 2,580,841 + CA265_RS10425 0.66 -0.5 2,580,841 + CA265_RS10425 0.66 +0.1 2,580,842 - CA265_RS10425 0.66 +1.0 2,580,904 + CA265_RS10425 0.68 -0.0 2,580,973 + CA265_RS10425 0.70 -0.2 2,580,974 - CA265_RS10425 0.70 +1.1 2,581,038 - CA265_RS10425 0.73 +0.2 2,581,038 - CA265_RS10425 0.73 +3.3 2,581,041 - CA265_RS10425 0.73 +1.0 2,581,073 + CA265_RS10425 0.74 -0.8 2,581,073 + CA265_RS10425 0.74 -0.2 2,581,074 - CA265_RS10425 0.74 +0.0 2,581,330 + CA265_RS10425 0.83 -0.1 2,581,331 - CA265_RS10425 0.83 +0.6 2,581,331 - CA265_RS10425 0.83 -0.2 2,581,345 + CA265_RS10425 0.84 +0.8 2,581,356 - CA265_RS10425 0.84 +2.1 2,581,397 - CA265_RS10425 0.86 +0.3 2,581,463 + CA265_RS10425 0.88 -0.8 2,581,463 + CA265_RS10425 0.88 -1.0 2,581,522 + +0.5 2,581,522 + +1.0 2,581,546 - +0.8 2,581,573 + -0.5 2,581,607 + +0.2 2,581,608 - +0.2 2,581,632 - -0.6 2,581,644 - -1.0 2,581,666 + -0.1 2,581,672 - +0.6 2,581,770 - +1.2 2,581,775 + -0.6 2,581,798 - -0.3 2,581,897 + -0.8 2,581,997 + +0.2 2,582,071 + CA265_RS10430 0.29 -0.8 2,582,071 + CA265_RS10430 0.29 +0.4 2,582,102 + CA265_RS10430 0.41 -2.0 2,582,116 + CA265_RS10430 0.46 -0.3 2,582,129 + CA265_RS10430 0.51 -0.2 2,582,171 + CA265_RS10430 0.68 -0.8 2,582,199 - CA265_RS10430 0.79 +0.2 2,582,251 - -0.2 2,582,355 + CA265_RS10435 0.44 -0.6 2,582,355 + CA265_RS10435 0.44 -0.5 2,582,356 - CA265_RS10435 0.45 -0.8 2,582,366 - CA265_RS10435 0.50 +0.7 2,582,380 + CA265_RS10435 0.57 -0.8 2,582,381 - CA265_RS10435 0.58 +0.8 2,582,387 - CA265_RS10435 0.61 +0.3 2,582,410 + CA265_RS10435 0.73 -1.9 2,582,440 - CA265_RS10435 0.89 +1.3 2,582,442 + -0.2 2,582,442 + -0.0 2,582,460 - +1.2 2,582,547 + +4.3 2,582,564 - -0.9 2,582,674 + -1.6 2,582,675 - -0.2 2,582,696 - +1.6 2,582,716 + +0.0 2,582,717 - -1.7 2,582,779 + -0.1 2,582,779 + -0.3 2,582,792 - +0.5 2,582,792 - -1.5
Or see this region's nucleotide sequence