Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS08530 and CA265_RS08535 overlap by 4 nucleotides CA265_RS08535 and CA265_RS08540 are separated by 166 nucleotides
CA265_RS08530: CA265_RS08530 - alpha-L-rhamnosidase, at 2,134,177 to 2,136,936
_RS08530
CA265_RS08535: CA265_RS08535 - glycosylase, at 2,136,933 to 2,138,045
_RS08535
CA265_RS08540: CA265_RS08540 - glycoside hydrolase family 2, at 2,138,212 to 2,140,620
_RS08540
Position (kb)
2136
2137
2138
2139 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2135.937 kb on - strand, within CA265_RS08530 at 2135.948 kb on - strand, within CA265_RS08530 at 2135.955 kb on + strand, within CA265_RS08530 at 2136.005 kb on - strand, within CA265_RS08530 at 2136.005 kb on - strand, within CA265_RS08530 at 2136.134 kb on - strand, within CA265_RS08530 at 2136.140 kb on + strand, within CA265_RS08530 at 2136.145 kb on - strand, within CA265_RS08530 at 2136.196 kb on + strand, within CA265_RS08530 at 2136.304 kb on + strand, within CA265_RS08530 at 2136.305 kb on - strand, within CA265_RS08530 at 2136.364 kb on - strand, within CA265_RS08530 at 2136.364 kb on - strand, within CA265_RS08530 at 2136.571 kb on - strand, within CA265_RS08530 at 2136.654 kb on + strand, within CA265_RS08530 at 2136.683 kb on + strand at 2136.699 kb on - strand at 2136.823 kb on + strand at 2136.823 kb on + strand at 2136.824 kb on - strand at 2136.841 kb on + strand at 2136.844 kb on - strand at 2136.910 kb on + strand at 2136.918 kb on + strand at 2136.929 kb on - strand at 2136.929 kb on - strand at 2137.055 kb on + strand, within CA265_RS08535 at 2137.055 kb on + strand, within CA265_RS08535 at 2137.056 kb on - strand, within CA265_RS08535 at 2137.056 kb on - strand, within CA265_RS08535 at 2137.060 kb on - strand, within CA265_RS08535 at 2137.085 kb on + strand, within CA265_RS08535 at 2137.112 kb on + strand, within CA265_RS08535 at 2137.119 kb on - strand, within CA265_RS08535 at 2137.138 kb on - strand, within CA265_RS08535 at 2137.223 kb on + strand, within CA265_RS08535 at 2137.223 kb on + strand, within CA265_RS08535 at 2137.278 kb on + strand, within CA265_RS08535 at 2137.278 kb on + strand, within CA265_RS08535 at 2137.279 kb on - strand, within CA265_RS08535 at 2137.281 kb on - strand, within CA265_RS08535 at 2137.321 kb on + strand, within CA265_RS08535 at 2137.322 kb on - strand, within CA265_RS08535 at 2137.322 kb on - strand, within CA265_RS08535 at 2137.337 kb on + strand, within CA265_RS08535 at 2137.338 kb on - strand, within CA265_RS08535 at 2137.354 kb on - strand, within CA265_RS08535 at 2137.385 kb on - strand, within CA265_RS08535 at 2137.410 kb on + strand, within CA265_RS08535 at 2137.413 kb on - strand, within CA265_RS08535 at 2137.413 kb on - strand, within CA265_RS08535 at 2137.433 kb on - strand, within CA265_RS08535 at 2137.469 kb on - strand, within CA265_RS08535 at 2137.489 kb on + strand, within CA265_RS08535 at 2137.490 kb on - strand, within CA265_RS08535 at 2137.490 kb on - strand at 2137.532 kb on - strand, within CA265_RS08535 at 2137.575 kb on + strand, within CA265_RS08535 at 2137.578 kb on + strand, within CA265_RS08535 at 2137.579 kb on - strand, within CA265_RS08535 at 2137.580 kb on + strand, within CA265_RS08535 at 2137.627 kb on - strand, within CA265_RS08535 at 2137.658 kb on + strand, within CA265_RS08535 at 2137.658 kb on + strand, within CA265_RS08535 at 2137.757 kb on - strand, within CA265_RS08535 at 2137.813 kb on + strand, within CA265_RS08535 at 2137.891 kb on + strand, within CA265_RS08535 at 2137.902 kb on - strand, within CA265_RS08535 at 2137.921 kb on - strand, within CA265_RS08535 at 2137.922 kb on + strand, within CA265_RS08535 at 2137.958 kb on + strand at 2138.018 kb on - strand at 2138.043 kb on + strand at 2138.054 kb on + strand at 2138.189 kb on + strand at 2138.244 kb on + strand at 2138.305 kb on + strand at 2138.305 kb on + strand at 2138.305 kb on + strand at 2138.314 kb on - strand at 2138.314 kb on - strand at 2138.316 kb on + strand at 2138.418 kb on - strand at 2138.463 kb on + strand, within CA265_RS08540 at 2138.469 kb on - strand, within CA265_RS08540 at 2138.492 kb on + strand, within CA265_RS08540 at 2138.497 kb on - strand, within CA265_RS08540 at 2138.527 kb on + strand, within CA265_RS08540 at 2138.551 kb on + strand, within CA265_RS08540 at 2138.605 kb on - strand, within CA265_RS08540 at 2138.605 kb on - strand, within CA265_RS08540 at 2138.651 kb on - strand, within CA265_RS08540 at 2138.679 kb on - strand, within CA265_RS08540 at 2138.737 kb on - strand, within CA265_RS08540 at 2138.739 kb on + strand, within CA265_RS08540 at 2138.741 kb on + strand, within CA265_RS08540 at 2138.799 kb on + strand, within CA265_RS08540 at 2138.800 kb on - strand, within CA265_RS08540 at 2138.854 kb on - strand, within CA265_RS08540 at 2138.857 kb on + strand, within CA265_RS08540
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 2,135,937 - CA265_RS08530 0.64 -0.6 2,135,948 - CA265_RS08530 0.64 -0.3 2,135,955 + CA265_RS08530 0.64 -0.2 2,136,005 - CA265_RS08530 0.66 -0.8 2,136,005 - CA265_RS08530 0.66 +0.1 2,136,134 - CA265_RS08530 0.71 -0.4 2,136,140 + CA265_RS08530 0.71 +0.2 2,136,145 - CA265_RS08530 0.71 +0.0 2,136,196 + CA265_RS08530 0.73 +0.2 2,136,304 + CA265_RS08530 0.77 +1.0 2,136,305 - CA265_RS08530 0.77 +0.2 2,136,364 - CA265_RS08530 0.79 +0.2 2,136,364 - CA265_RS08530 0.79 -0.4 2,136,571 - CA265_RS08530 0.87 -0.2 2,136,654 + CA265_RS08530 0.90 +0.3 2,136,683 + +0.4 2,136,699 - +0.5 2,136,823 + -1.3 2,136,823 + -0.4 2,136,824 - +0.2 2,136,841 + +0.1 2,136,844 - +0.0 2,136,910 + +0.5 2,136,918 + -0.5 2,136,929 - +0.2 2,136,929 - +0.8 2,137,055 + CA265_RS08535 0.11 -0.5 2,137,055 + CA265_RS08535 0.11 +0.5 2,137,056 - CA265_RS08535 0.11 +1.3 2,137,056 - CA265_RS08535 0.11 -0.3 2,137,060 - CA265_RS08535 0.11 -1.6 2,137,085 + CA265_RS08535 0.14 -0.3 2,137,112 + CA265_RS08535 0.16 +0.3 2,137,119 - CA265_RS08535 0.17 +0.3 2,137,138 - CA265_RS08535 0.18 -0.6 2,137,223 + CA265_RS08535 0.26 -1.3 2,137,223 + CA265_RS08535 0.26 +0.2 2,137,278 + CA265_RS08535 0.31 +0.6 2,137,278 + CA265_RS08535 0.31 -1.5 2,137,279 - CA265_RS08535 0.31 -0.2 2,137,281 - CA265_RS08535 0.31 +0.4 2,137,321 + CA265_RS08535 0.35 -0.2 2,137,322 - CA265_RS08535 0.35 +0.4 2,137,322 - CA265_RS08535 0.35 -0.8 2,137,337 + CA265_RS08535 0.36 -0.6 2,137,338 - CA265_RS08535 0.36 +0.4 2,137,354 - CA265_RS08535 0.38 -0.2 2,137,385 - CA265_RS08535 0.41 -0.9 2,137,410 + CA265_RS08535 0.43 -0.7 2,137,413 - CA265_RS08535 0.43 +0.2 2,137,413 - CA265_RS08535 0.43 +0.4 2,137,433 - CA265_RS08535 0.45 -1.3 2,137,469 - CA265_RS08535 0.48 -0.6 2,137,489 + CA265_RS08535 0.50 +0.2 2,137,490 - CA265_RS08535 0.50 +0.1 2,137,490 - +1.4 2,137,532 - CA265_RS08535 0.54 -0.8 2,137,575 + CA265_RS08535 0.58 +1.1 2,137,578 + CA265_RS08535 0.58 +0.4 2,137,579 - CA265_RS08535 0.58 +0.8 2,137,580 + CA265_RS08535 0.58 -0.2 2,137,627 - CA265_RS08535 0.62 -2.5 2,137,658 + CA265_RS08535 0.65 +0.3 2,137,658 + CA265_RS08535 0.65 +0.2 2,137,757 - CA265_RS08535 0.74 -0.4 2,137,813 + CA265_RS08535 0.79 -0.9 2,137,891 + CA265_RS08535 0.86 +0.6 2,137,902 - CA265_RS08535 0.87 +0.1 2,137,921 - CA265_RS08535 0.89 -1.3 2,137,922 + CA265_RS08535 0.89 -0.1 2,137,958 + +0.0 2,138,018 - +0.9 2,138,043 + -3.3 2,138,054 + +0.3 2,138,189 + +0.4 2,138,244 + +0.3 2,138,305 + +0.0 2,138,305 + +0.7 2,138,305 + -0.8 2,138,314 - -1.0 2,138,314 - +0.6 2,138,316 + +1.0 2,138,418 - +1.0 2,138,463 + CA265_RS08540 0.10 +0.3 2,138,469 - CA265_RS08540 0.11 +0.5 2,138,492 + CA265_RS08540 0.12 +0.6 2,138,497 - CA265_RS08540 0.12 +0.9 2,138,527 + CA265_RS08540 0.13 -0.1 2,138,551 + CA265_RS08540 0.14 +0.1 2,138,605 - CA265_RS08540 0.16 +0.1 2,138,605 - CA265_RS08540 0.16 +0.8 2,138,651 - CA265_RS08540 0.18 +0.2 2,138,679 - CA265_RS08540 0.19 -1.2 2,138,737 - CA265_RS08540 0.22 -0.0 2,138,739 + CA265_RS08540 0.22 +1.4 2,138,741 + CA265_RS08540 0.22 -0.1 2,138,799 + CA265_RS08540 0.24 -0.0 2,138,800 - CA265_RS08540 0.24 -0.6 2,138,854 - CA265_RS08540 0.27 -1.4 2,138,857 + CA265_RS08540 0.27 -0.9
Or see this region's nucleotide sequence