Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS08320 and CA265_RS08325 are separated by 1 nucleotides CA265_RS08325 and CA265_RS08330 are separated by 291 nucleotides CA265_RS08330 and CA265_RS08335 are separated by 102 nucleotides
CA265_RS08320: CA265_RS08320 - DNA mismatch repair protein MutT, at 2,062,464 to 2,063,162
_RS08320
CA265_RS08325: CA265_RS08325 - haloacid dehalogenase, at 2,063,164 to 2,063,835
_RS08325
CA265_RS08330: CA265_RS08330 - galactose-1-epimerase, at 2,064,127 to 2,065,278
_RS08330
CA265_RS08335: CA265_RS08335 - alpha-hydroxy-acid oxidizing enzyme, at 2,065,381 to 2,066,550
_RS08335
Position (kb)
2064
2065
2066 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2063.165 kb on + strand at 2063.170 kb on + strand at 2063.184 kb on + strand at 2063.185 kb on - strand at 2063.207 kb on + strand at 2063.213 kb on - strand at 2063.213 kb on - strand at 2063.263 kb on + strand, within CA265_RS08325 at 2063.299 kb on + strand, within CA265_RS08325 at 2063.347 kb on - strand, within CA265_RS08325 at 2063.442 kb on + strand, within CA265_RS08325 at 2063.473 kb on - strand, within CA265_RS08325 at 2063.604 kb on + strand, within CA265_RS08325 at 2063.604 kb on + strand, within CA265_RS08325 at 2063.618 kb on - strand, within CA265_RS08325 at 2063.637 kb on + strand, within CA265_RS08325 at 2063.681 kb on + strand, within CA265_RS08325 at 2063.742 kb on + strand, within CA265_RS08325 at 2063.742 kb on + strand, within CA265_RS08325 at 2063.744 kb on + strand, within CA265_RS08325 at 2063.757 kb on + strand, within CA265_RS08325 at 2063.762 kb on + strand, within CA265_RS08325 at 2063.830 kb on - strand at 2063.850 kb on - strand at 2063.904 kb on + strand at 2063.968 kb on - strand at 2063.990 kb on + strand at 2064.162 kb on + strand at 2064.187 kb on - strand at 2064.251 kb on - strand, within CA265_RS08330 at 2064.288 kb on + strand, within CA265_RS08330 at 2064.289 kb on - strand, within CA265_RS08330 at 2064.405 kb on + strand, within CA265_RS08330 at 2064.514 kb on - strand, within CA265_RS08330 at 2064.518 kb on + strand, within CA265_RS08330 at 2064.526 kb on - strand, within CA265_RS08330 at 2064.597 kb on - strand, within CA265_RS08330 at 2064.622 kb on + strand, within CA265_RS08330 at 2064.674 kb on + strand, within CA265_RS08330 at 2064.674 kb on + strand, within CA265_RS08330 at 2064.675 kb on - strand, within CA265_RS08330 at 2064.716 kb on - strand, within CA265_RS08330 at 2064.726 kb on + strand, within CA265_RS08330 at 2064.727 kb on - strand, within CA265_RS08330 at 2064.766 kb on + strand, within CA265_RS08330 at 2064.837 kb on + strand, within CA265_RS08330 at 2064.837 kb on + strand, within CA265_RS08330 at 2064.852 kb on + strand, within CA265_RS08330 at 2064.852 kb on + strand, within CA265_RS08330 at 2064.859 kb on - strand, within CA265_RS08330 at 2064.871 kb on - strand, within CA265_RS08330 at 2064.871 kb on - strand, within CA265_RS08330 at 2065.144 kb on + strand, within CA265_RS08330 at 2065.228 kb on - strand at 2065.261 kb on + strand at 2065.282 kb on + strand at 2065.282 kb on + strand at 2065.283 kb on - strand at 2065.382 kb on + strand at 2065.390 kb on + strand at 2065.430 kb on - strand at 2065.430 kb on - strand at 2065.451 kb on - strand at 2065.467 kb on + strand at 2065.474 kb on - strand at 2065.514 kb on + strand, within CA265_RS08335 at 2065.591 kb on - strand, within CA265_RS08335 at 2065.591 kb on - strand, within CA265_RS08335 at 2065.625 kb on + strand, within CA265_RS08335 at 2065.625 kb on + strand, within CA265_RS08335 at 2065.625 kb on + strand, within CA265_RS08335 at 2065.626 kb on - strand, within CA265_RS08335 at 2065.626 kb on - strand, within CA265_RS08335 at 2065.626 kb on - strand, within CA265_RS08335 at 2065.626 kb on - strand, within CA265_RS08335 at 2065.628 kb on - strand, within CA265_RS08335 at 2065.711 kb on + strand, within CA265_RS08335 at 2065.746 kb on + strand, within CA265_RS08335 at 2065.746 kb on + strand, within CA265_RS08335 at 2065.891 kb on - strand, within CA265_RS08335 at 2065.892 kb on + strand, within CA265_RS08335 at 2066.002 kb on - strand, within CA265_RS08335 at 2066.027 kb on + strand, within CA265_RS08335 at 2066.098 kb on + strand, within CA265_RS08335 at 2066.099 kb on - strand, within CA265_RS08335 at 2066.103 kb on + strand, within CA265_RS08335 at 2066.103 kb on + strand, within CA265_RS08335 at 2066.104 kb on - strand, within CA265_RS08335 at 2066.135 kb on + strand, within CA265_RS08335 at 2066.164 kb on + strand, within CA265_RS08335 at 2066.165 kb on - strand, within CA265_RS08335 at 2066.174 kb on - strand, within CA265_RS08335 at 2066.187 kb on + strand, within CA265_RS08335 at 2066.188 kb on - strand, within CA265_RS08335 at 2066.202 kb on + strand, within CA265_RS08335 at 2066.206 kb on + strand, within CA265_RS08335 at 2066.211 kb on + strand at 2066.230 kb on - strand, within CA265_RS08335
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 2,063,165 + +0.4 2,063,170 + +0.2 2,063,184 + -1.2 2,063,185 - +0.5 2,063,207 + +0.0 2,063,213 - +0.1 2,063,213 - +1.6 2,063,263 + CA265_RS08325 0.15 +0.4 2,063,299 + CA265_RS08325 0.20 +0.1 2,063,347 - CA265_RS08325 0.27 -0.8 2,063,442 + CA265_RS08325 0.41 -0.2 2,063,473 - CA265_RS08325 0.46 +2.2 2,063,604 + CA265_RS08325 0.65 -0.4 2,063,604 + CA265_RS08325 0.65 +0.6 2,063,618 - CA265_RS08325 0.68 +0.8 2,063,637 + CA265_RS08325 0.70 -0.4 2,063,681 + CA265_RS08325 0.77 +0.1 2,063,742 + CA265_RS08325 0.86 -0.8 2,063,742 + CA265_RS08325 0.86 +0.3 2,063,744 + CA265_RS08325 0.86 +1.2 2,063,757 + CA265_RS08325 0.88 +1.4 2,063,762 + CA265_RS08325 0.89 -0.3 2,063,830 - +0.5 2,063,850 - +0.3 2,063,904 + +0.3 2,063,968 - +0.6 2,063,990 + +1.1 2,064,162 + +0.6 2,064,187 - -0.3 2,064,251 - CA265_RS08330 0.11 +2.1 2,064,288 + CA265_RS08330 0.14 +0.0 2,064,289 - CA265_RS08330 0.14 -0.0 2,064,405 + CA265_RS08330 0.24 -0.1 2,064,514 - CA265_RS08330 0.34 -0.6 2,064,518 + CA265_RS08330 0.34 +1.1 2,064,526 - CA265_RS08330 0.35 +0.8 2,064,597 - CA265_RS08330 0.41 +0.5 2,064,622 + CA265_RS08330 0.43 +1.1 2,064,674 + CA265_RS08330 0.47 +1.2 2,064,674 + CA265_RS08330 0.47 -0.1 2,064,675 - CA265_RS08330 0.48 -0.4 2,064,716 - CA265_RS08330 0.51 +1.7 2,064,726 + CA265_RS08330 0.52 +0.3 2,064,727 - CA265_RS08330 0.52 -0.1 2,064,766 + CA265_RS08330 0.55 -0.1 2,064,837 + CA265_RS08330 0.62 +0.7 2,064,837 + CA265_RS08330 0.62 -0.2 2,064,852 + CA265_RS08330 0.63 +0.1 2,064,852 + CA265_RS08330 0.63 +1.4 2,064,859 - CA265_RS08330 0.64 -1.0 2,064,871 - CA265_RS08330 0.65 -0.1 2,064,871 - CA265_RS08330 0.65 -0.2 2,065,144 + CA265_RS08330 0.88 +1.6 2,065,228 - -1.2 2,065,261 + +0.5 2,065,282 + +3.1 2,065,282 + -0.6 2,065,283 - +1.2 2,065,382 + +0.0 2,065,390 + -0.5 2,065,430 - -1.1 2,065,430 - +0.7 2,065,451 - +0.7 2,065,467 + -0.5 2,065,474 - -0.6 2,065,514 + CA265_RS08335 0.11 -0.1 2,065,591 - CA265_RS08335 0.18 +0.8 2,065,591 - CA265_RS08335 0.18 +0.2 2,065,625 + CA265_RS08335 0.21 +1.4 2,065,625 + CA265_RS08335 0.21 +0.3 2,065,625 + CA265_RS08335 0.21 -0.5 2,065,626 - CA265_RS08335 0.21 +0.6 2,065,626 - CA265_RS08335 0.21 -0.3 2,065,626 - CA265_RS08335 0.21 +1.2 2,065,626 - CA265_RS08335 0.21 +1.5 2,065,628 - CA265_RS08335 0.21 +0.9 2,065,711 + CA265_RS08335 0.28 +0.2 2,065,746 + CA265_RS08335 0.31 +0.7 2,065,746 + CA265_RS08335 0.31 +0.7 2,065,891 - CA265_RS08335 0.44 +0.5 2,065,892 + CA265_RS08335 0.44 -0.1 2,066,002 - CA265_RS08335 0.53 +1.4 2,066,027 + CA265_RS08335 0.55 -0.2 2,066,098 + CA265_RS08335 0.61 -1.1 2,066,099 - CA265_RS08335 0.61 +1.1 2,066,103 + CA265_RS08335 0.62 -0.0 2,066,103 + CA265_RS08335 0.62 +0.1 2,066,104 - CA265_RS08335 0.62 +0.6 2,066,135 + CA265_RS08335 0.64 +0.5 2,066,164 + CA265_RS08335 0.67 +1.3 2,066,165 - CA265_RS08335 0.67 -0.5 2,066,174 - CA265_RS08335 0.68 +0.7 2,066,187 + CA265_RS08335 0.69 +0.2 2,066,188 - CA265_RS08335 0.69 +0.0 2,066,202 + CA265_RS08335 0.70 -0.2 2,066,206 + CA265_RS08335 0.71 +0.3 2,066,211 + +0.1 2,066,230 - CA265_RS08335 0.73 -1.1
Or see this region's nucleotide sequence