Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS08315 and CA265_RS08320 are separated by 180 nucleotides CA265_RS08320 and CA265_RS08325 are separated by 1 nucleotides CA265_RS08325 and CA265_RS08330 are separated by 291 nucleotides
CA265_RS08315: CA265_RS08315 - tRNA-Lys, at 2,062,211 to 2,062,283
_RS08315
CA265_RS08320: CA265_RS08320 - DNA mismatch repair protein MutT, at 2,062,464 to 2,063,162
_RS08320
CA265_RS08325: CA265_RS08325 - haloacid dehalogenase, at 2,063,164 to 2,063,835
_RS08325
CA265_RS08330: CA265_RS08330 - galactose-1-epimerase, at 2,064,127 to 2,065,278
_RS08330
Position (kb)
2063
2064 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2062.167 kb on + strand at 2062.242 kb on + strand, within CA265_RS08315 at 2062.298 kb on - strand at 2062.465 kb on + strand at 2062.484 kb on + strand at 2062.521 kb on - strand at 2062.593 kb on + strand, within CA265_RS08320 at 2062.663 kb on - strand, within CA265_RS08320 at 2062.686 kb on - strand, within CA265_RS08320 at 2062.844 kb on + strand, within CA265_RS08320 at 2062.850 kb on - strand at 2062.850 kb on - strand, within CA265_RS08320 at 2062.853 kb on + strand, within CA265_RS08320 at 2062.854 kb on - strand, within CA265_RS08320 at 2062.856 kb on - strand, within CA265_RS08320 at 2062.877 kb on - strand, within CA265_RS08320 at 2062.919 kb on + strand, within CA265_RS08320 at 2062.974 kb on + strand, within CA265_RS08320 at 2063.019 kb on - strand, within CA265_RS08320 at 2063.165 kb on + strand at 2063.170 kb on + strand at 2063.184 kb on + strand at 2063.185 kb on - strand at 2063.207 kb on + strand at 2063.213 kb on - strand at 2063.213 kb on - strand at 2063.263 kb on + strand, within CA265_RS08325 at 2063.299 kb on + strand, within CA265_RS08325 at 2063.347 kb on - strand, within CA265_RS08325 at 2063.442 kb on + strand, within CA265_RS08325 at 2063.473 kb on - strand, within CA265_RS08325 at 2063.604 kb on + strand, within CA265_RS08325 at 2063.604 kb on + strand, within CA265_RS08325 at 2063.618 kb on - strand, within CA265_RS08325 at 2063.637 kb on + strand, within CA265_RS08325 at 2063.681 kb on + strand, within CA265_RS08325 at 2063.742 kb on + strand, within CA265_RS08325 at 2063.742 kb on + strand, within CA265_RS08325 at 2063.744 kb on + strand, within CA265_RS08325 at 2063.757 kb on + strand, within CA265_RS08325 at 2063.762 kb on + strand, within CA265_RS08325 at 2063.830 kb on - strand at 2063.850 kb on - strand at 2063.904 kb on + strand at 2063.968 kb on - strand at 2063.990 kb on + strand at 2064.162 kb on + strand at 2064.187 kb on - strand at 2064.251 kb on - strand, within CA265_RS08330 at 2064.288 kb on + strand, within CA265_RS08330 at 2064.289 kb on - strand, within CA265_RS08330 at 2064.405 kb on + strand, within CA265_RS08330 at 2064.514 kb on - strand, within CA265_RS08330 at 2064.518 kb on + strand, within CA265_RS08330 at 2064.526 kb on - strand, within CA265_RS08330 at 2064.597 kb on - strand, within CA265_RS08330 at 2064.622 kb on + strand, within CA265_RS08330 at 2064.674 kb on + strand, within CA265_RS08330 at 2064.674 kb on + strand, within CA265_RS08330 at 2064.675 kb on - strand, within CA265_RS08330 at 2064.716 kb on - strand, within CA265_RS08330 at 2064.726 kb on + strand, within CA265_RS08330 at 2064.727 kb on - strand, within CA265_RS08330 at 2064.766 kb on + strand, within CA265_RS08330
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 2,062,167 + -0.9 2,062,242 + CA265_RS08315 0.42 +0.1 2,062,298 - -1.9 2,062,465 + +0.3 2,062,484 + -0.4 2,062,521 - +0.2 2,062,593 + CA265_RS08320 0.18 -0.1 2,062,663 - CA265_RS08320 0.28 +0.4 2,062,686 - CA265_RS08320 0.32 +0.2 2,062,844 + CA265_RS08320 0.54 +0.4 2,062,850 - +2.4 2,062,850 - CA265_RS08320 0.55 -0.2 2,062,853 + CA265_RS08320 0.56 +0.8 2,062,854 - CA265_RS08320 0.56 +0.5 2,062,856 - CA265_RS08320 0.56 +0.3 2,062,877 - CA265_RS08320 0.59 +0.1 2,062,919 + CA265_RS08320 0.65 -0.0 2,062,974 + CA265_RS08320 0.73 +0.0 2,063,019 - CA265_RS08320 0.79 -0.9 2,063,165 + +0.4 2,063,170 + +0.2 2,063,184 + -1.2 2,063,185 - +0.5 2,063,207 + +0.0 2,063,213 - +0.1 2,063,213 - +1.6 2,063,263 + CA265_RS08325 0.15 +0.4 2,063,299 + CA265_RS08325 0.20 +0.1 2,063,347 - CA265_RS08325 0.27 -0.8 2,063,442 + CA265_RS08325 0.41 -0.2 2,063,473 - CA265_RS08325 0.46 +2.2 2,063,604 + CA265_RS08325 0.65 -0.4 2,063,604 + CA265_RS08325 0.65 +0.6 2,063,618 - CA265_RS08325 0.68 +0.8 2,063,637 + CA265_RS08325 0.70 -0.4 2,063,681 + CA265_RS08325 0.77 +0.1 2,063,742 + CA265_RS08325 0.86 -0.8 2,063,742 + CA265_RS08325 0.86 +0.3 2,063,744 + CA265_RS08325 0.86 +1.2 2,063,757 + CA265_RS08325 0.88 +1.4 2,063,762 + CA265_RS08325 0.89 -0.3 2,063,830 - +0.5 2,063,850 - +0.3 2,063,904 + +0.3 2,063,968 - +0.6 2,063,990 + +1.1 2,064,162 + +0.6 2,064,187 - -0.3 2,064,251 - CA265_RS08330 0.11 +2.1 2,064,288 + CA265_RS08330 0.14 +0.0 2,064,289 - CA265_RS08330 0.14 -0.0 2,064,405 + CA265_RS08330 0.24 -0.1 2,064,514 - CA265_RS08330 0.34 -0.6 2,064,518 + CA265_RS08330 0.34 +1.1 2,064,526 - CA265_RS08330 0.35 +0.8 2,064,597 - CA265_RS08330 0.41 +0.5 2,064,622 + CA265_RS08330 0.43 +1.1 2,064,674 + CA265_RS08330 0.47 +1.2 2,064,674 + CA265_RS08330 0.47 -0.1 2,064,675 - CA265_RS08330 0.48 -0.4 2,064,716 - CA265_RS08330 0.51 +1.7 2,064,726 + CA265_RS08330 0.52 +0.3 2,064,727 - CA265_RS08330 0.52 -0.1 2,064,766 + CA265_RS08330 0.55 -0.1
Or see this region's nucleotide sequence