Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS08105 and CA265_RS08110 are separated by 54 nucleotides CA265_RS08110 and CA265_RS08115 are separated by 101 nucleotides CA265_RS08115 and CA265_RS08120 are separated by 403 nucleotides
CA265_RS08105: CA265_RS08105 - DUF983 domain-containing protein, at 2,013,283 to 2,013,675
_RS08105
CA265_RS08110: CA265_RS08110 - hypothetical protein, at 2,013,730 to 2,014,254
_RS08110
CA265_RS08115: CA265_RS08115 - hypothetical protein, at 2,014,356 to 2,014,817
_RS08115
CA265_RS08120: CA265_RS08120 - copper homeostasis protein CutC, at 2,015,221 to 2,015,970
_RS08120
Position (kb)
2014
2015 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2013.365 kb on + strand, within CA265_RS08105 at 2013.397 kb on - strand, within CA265_RS08105 at 2013.467 kb on - strand, within CA265_RS08105 at 2013.551 kb on - strand, within CA265_RS08105 at 2013.551 kb on - strand, within CA265_RS08105 at 2013.554 kb on - strand, within CA265_RS08105 at 2013.554 kb on - strand, within CA265_RS08105 at 2013.581 kb on + strand, within CA265_RS08105 at 2013.587 kb on + strand, within CA265_RS08105 at 2013.631 kb on + strand, within CA265_RS08105 at 2013.631 kb on + strand, within CA265_RS08105 at 2013.653 kb on - strand at 2013.681 kb on + strand at 2013.683 kb on + strand at 2013.684 kb on - strand at 2013.687 kb on + strand at 2013.731 kb on + strand at 2013.732 kb on - strand at 2013.753 kb on + strand at 2013.753 kb on + strand at 2013.755 kb on + strand at 2013.757 kb on - strand at 2013.802 kb on - strand, within CA265_RS08110 at 2013.988 kb on - strand, within CA265_RS08110 at 2014.143 kb on + strand, within CA265_RS08110 at 2014.145 kb on + strand, within CA265_RS08110 at 2014.176 kb on + strand, within CA265_RS08110 at 2014.225 kb on - strand at 2014.299 kb on + strand at 2014.392 kb on + strand at 2014.401 kb on + strand at 2014.404 kb on - strand, within CA265_RS08115 at 2014.507 kb on - strand, within CA265_RS08115 at 2014.552 kb on + strand, within CA265_RS08115 at 2014.604 kb on + strand, within CA265_RS08115 at 2014.639 kb on + strand, within CA265_RS08115 at 2014.640 kb on - strand, within CA265_RS08115 at 2014.641 kb on + strand, within CA265_RS08115 at 2014.663 kb on - strand, within CA265_RS08115 at 2014.702 kb on + strand, within CA265_RS08115 at 2014.706 kb on - strand, within CA265_RS08115 at 2014.779 kb on + strand at 2014.794 kb on - strand at 2014.795 kb on + strand at 2014.850 kb on - strand at 2014.884 kb on - strand at 2015.064 kb on + strand at 2015.084 kb on - strand at 2015.091 kb on + strand at 2015.241 kb on + strand at 2015.291 kb on + strand at 2015.292 kb on - strand at 2015.303 kb on - strand, within CA265_RS08120 at 2015.303 kb on - strand, within CA265_RS08120 at 2015.303 kb on - strand, within CA265_RS08120 at 2015.305 kb on - strand, within CA265_RS08120 at 2015.443 kb on - strand, within CA265_RS08120 at 2015.448 kb on + strand, within CA265_RS08120 at 2015.531 kb on - strand, within CA265_RS08120 at 2015.531 kb on - strand, within CA265_RS08120 at 2015.554 kb on + strand, within CA265_RS08120 at 2015.555 kb on - strand, within CA265_RS08120 at 2015.589 kb on + strand, within CA265_RS08120 at 2015.611 kb on + strand, within CA265_RS08120 at 2015.611 kb on + strand, within CA265_RS08120 at 2015.612 kb on - strand, within CA265_RS08120 at 2015.691 kb on + strand, within CA265_RS08120 at 2015.710 kb on + strand, within CA265_RS08120 at 2015.791 kb on + strand, within CA265_RS08120 at 2015.791 kb on + strand, within CA265_RS08120 at 2015.791 kb on + strand, within CA265_RS08120 at 2015.791 kb on + strand, within CA265_RS08120 at 2015.793 kb on + strand, within CA265_RS08120 at 2015.793 kb on + strand, within CA265_RS08120 at 2015.812 kb on - strand, within CA265_RS08120
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 2,013,365 + CA265_RS08105 0.21 +0.4 2,013,397 - CA265_RS08105 0.29 -2.2 2,013,467 - CA265_RS08105 0.47 +0.6 2,013,551 - CA265_RS08105 0.68 -0.1 2,013,551 - CA265_RS08105 0.68 -2.1 2,013,554 - CA265_RS08105 0.69 -1.0 2,013,554 - CA265_RS08105 0.69 -1.4 2,013,581 + CA265_RS08105 0.76 +0.7 2,013,587 + CA265_RS08105 0.77 -0.2 2,013,631 + CA265_RS08105 0.89 +0.5 2,013,631 + CA265_RS08105 0.89 +1.0 2,013,653 - -0.0 2,013,681 + -0.6 2,013,683 + -0.3 2,013,684 - -0.2 2,013,687 + +0.4 2,013,731 + +0.2 2,013,732 - +0.6 2,013,753 + -0.3 2,013,753 + -0.9 2,013,755 + -0.1 2,013,757 - -0.6 2,013,802 - CA265_RS08110 0.14 +0.5 2,013,988 - CA265_RS08110 0.49 +0.9 2,014,143 + CA265_RS08110 0.79 +1.0 2,014,145 + CA265_RS08110 0.79 +0.4 2,014,176 + CA265_RS08110 0.85 -0.4 2,014,225 - -0.3 2,014,299 + +0.8 2,014,392 + -0.5 2,014,401 + +0.8 2,014,404 - CA265_RS08115 0.10 +0.6 2,014,507 - CA265_RS08115 0.33 +0.7 2,014,552 + CA265_RS08115 0.42 +2.3 2,014,604 + CA265_RS08115 0.54 -0.1 2,014,639 + CA265_RS08115 0.61 -0.8 2,014,640 - CA265_RS08115 0.61 +1.1 2,014,641 + CA265_RS08115 0.62 +1.3 2,014,663 - CA265_RS08115 0.66 -0.1 2,014,702 + CA265_RS08115 0.75 +0.6 2,014,706 - CA265_RS08115 0.76 +0.1 2,014,779 + -0.3 2,014,794 - -1.0 2,014,795 + +1.2 2,014,850 - -0.7 2,014,884 - -0.7 2,015,064 + -0.2 2,015,084 - -0.2 2,015,091 + +0.2 2,015,241 + -1.6 2,015,291 + -1.1 2,015,292 - -0.2 2,015,303 - CA265_RS08120 0.11 -0.5 2,015,303 - CA265_RS08120 0.11 -0.9 2,015,303 - CA265_RS08120 0.11 -0.9 2,015,305 - CA265_RS08120 0.11 -1.1 2,015,443 - CA265_RS08120 0.30 -0.5 2,015,448 + CA265_RS08120 0.30 +0.0 2,015,531 - CA265_RS08120 0.41 -1.9 2,015,531 - CA265_RS08120 0.41 -0.8 2,015,554 + CA265_RS08120 0.44 -1.7 2,015,555 - CA265_RS08120 0.45 -0.6 2,015,589 + CA265_RS08120 0.49 -0.6 2,015,611 + CA265_RS08120 0.52 -1.3 2,015,611 + CA265_RS08120 0.52 -1.2 2,015,612 - CA265_RS08120 0.52 -0.3 2,015,691 + CA265_RS08120 0.63 -0.5 2,015,710 + CA265_RS08120 0.65 -1.1 2,015,791 + CA265_RS08120 0.76 -1.0 2,015,791 + CA265_RS08120 0.76 -1.0 2,015,791 + CA265_RS08120 0.76 -0.7 2,015,791 + CA265_RS08120 0.76 -0.2 2,015,793 + CA265_RS08120 0.76 -0.5 2,015,793 + CA265_RS08120 0.76 -1.6 2,015,812 - CA265_RS08120 0.79 -0.6
Or see this region's nucleotide sequence