Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS08050 and CA265_RS08055 are separated by 267 nucleotides CA265_RS08055 and CA265_RS08060 are separated by 350 nucleotides CA265_RS08060 and CA265_RS08065 are separated by 68 nucleotides
CA265_RS08050: CA265_RS08050 - hypothetical protein, at 2,004,633 to 2,005,178
_RS08050
CA265_RS08055: CA265_RS08055 - hypothetical protein, at 2,005,446 to 2,006,282
_RS08055
CA265_RS08060: CA265_RS08060 - hypothetical protein, at 2,006,633 to 2,006,947
_RS08060
CA265_RS08065: CA265_RS08065 - hypothetical protein, at 2,007,016 to 2,007,663
_RS08065
Position (kb)
2005
2006
2007 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2004.452 kb on + strand at 2004.524 kb on - strand at 2004.526 kb on + strand at 2004.526 kb on + strand at 2004.527 kb on - strand at 2004.527 kb on - strand at 2004.605 kb on + strand at 2004.689 kb on - strand, within CA265_RS08050 at 2004.776 kb on + strand, within CA265_RS08050 at 2004.832 kb on + strand, within CA265_RS08050 at 2004.960 kb on + strand, within CA265_RS08050 at 2004.968 kb on - strand, within CA265_RS08050 at 2004.994 kb on + strand, within CA265_RS08050 at 2005.063 kb on + strand, within CA265_RS08050 at 2005.188 kb on - strand at 2005.195 kb on - strand at 2005.217 kb on + strand at 2005.350 kb on - strand at 2005.418 kb on - strand at 2005.419 kb on + strand at 2005.419 kb on + strand at 2005.419 kb on + strand at 2005.420 kb on - strand at 2005.420 kb on - strand at 2005.420 kb on - strand at 2005.423 kb on + strand at 2005.425 kb on + strand at 2005.481 kb on - strand at 2005.575 kb on - strand, within CA265_RS08055 at 2005.575 kb on - strand, within CA265_RS08055 at 2005.580 kb on - strand, within CA265_RS08055 at 2005.650 kb on + strand, within CA265_RS08055 at 2005.650 kb on + strand, within CA265_RS08055 at 2005.742 kb on + strand, within CA265_RS08055 at 2005.742 kb on + strand, within CA265_RS08055 at 2005.747 kb on - strand, within CA265_RS08055 at 2005.748 kb on + strand, within CA265_RS08055 at 2005.748 kb on + strand, within CA265_RS08055 at 2005.760 kb on + strand, within CA265_RS08055 at 2005.879 kb on + strand, within CA265_RS08055 at 2005.979 kb on - strand, within CA265_RS08055 at 2006.007 kb on - strand, within CA265_RS08055 at 2006.007 kb on - strand, within CA265_RS08055 at 2006.075 kb on + strand, within CA265_RS08055 at 2006.140 kb on - strand, within CA265_RS08055 at 2006.141 kb on + strand, within CA265_RS08055 at 2006.294 kb on - strand at 2006.318 kb on + strand at 2006.351 kb on - strand at 2006.351 kb on - strand at 2006.354 kb on - strand at 2006.443 kb on + strand at 2006.444 kb on - strand at 2006.444 kb on - strand at 2006.450 kb on - strand at 2006.451 kb on + strand at 2006.455 kb on + strand at 2006.467 kb on - strand at 2006.512 kb on - strand at 2006.512 kb on - strand at 2006.630 kb on - strand at 2006.630 kb on - strand at 2006.633 kb on - strand at 2006.633 kb on - strand at 2006.642 kb on + strand at 2006.763 kb on + strand, within CA265_RS08060 at 2006.840 kb on - strand, within CA265_RS08060 at 2006.897 kb on + strand, within CA265_RS08060 at 2006.952 kb on + strand at 2006.990 kb on + strand at 2006.999 kb on - strand at 2006.999 kb on - strand at 2007.005 kb on + strand at 2007.006 kb on - strand at 2007.033 kb on + strand at 2007.034 kb on - strand at 2007.062 kb on + strand at 2007.063 kb on - strand at 2007.072 kb on + strand at 2007.073 kb on - strand at 2007.102 kb on - strand, within CA265_RS08065 at 2007.102 kb on - strand, within CA265_RS08065 at 2007.130 kb on - strand, within CA265_RS08065 at 2007.230 kb on - strand, within CA265_RS08065 at 2007.239 kb on - strand, within CA265_RS08065 at 2007.239 kb on - strand, within CA265_RS08065 at 2007.242 kb on - strand, within CA265_RS08065 at 2007.253 kb on - strand, within CA265_RS08065
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 2,004,452 + +0.2 2,004,524 - -0.1 2,004,526 + +0.5 2,004,526 + -0.1 2,004,527 - -0.4 2,004,527 - +0.7 2,004,605 + -1.0 2,004,689 - CA265_RS08050 0.10 -0.8 2,004,776 + CA265_RS08050 0.26 +0.5 2,004,832 + CA265_RS08050 0.36 +0.5 2,004,960 + CA265_RS08050 0.60 +0.2 2,004,968 - CA265_RS08050 0.61 -0.4 2,004,994 + CA265_RS08050 0.66 +0.3 2,005,063 + CA265_RS08050 0.79 +0.6 2,005,188 - -0.5 2,005,195 - -1.3 2,005,217 + -0.2 2,005,350 - +0.9 2,005,418 - -0.4 2,005,419 + -0.0 2,005,419 + -0.8 2,005,419 + -1.7 2,005,420 - +0.3 2,005,420 - -0.2 2,005,420 - -0.5 2,005,423 + +0.3 2,005,425 + -0.1 2,005,481 - -1.2 2,005,575 - CA265_RS08055 0.15 -0.2 2,005,575 - CA265_RS08055 0.15 +0.6 2,005,580 - CA265_RS08055 0.16 +0.8 2,005,650 + CA265_RS08055 0.24 +0.5 2,005,650 + CA265_RS08055 0.24 -0.5 2,005,742 + CA265_RS08055 0.35 +0.4 2,005,742 + CA265_RS08055 0.35 +1.0 2,005,747 - CA265_RS08055 0.36 +1.4 2,005,748 + CA265_RS08055 0.36 -0.5 2,005,748 + CA265_RS08055 0.36 +2.4 2,005,760 + CA265_RS08055 0.38 -0.5 2,005,879 + CA265_RS08055 0.52 +2.1 2,005,979 - CA265_RS08055 0.64 -0.3 2,006,007 - CA265_RS08055 0.67 -2.1 2,006,007 - CA265_RS08055 0.67 -0.2 2,006,075 + CA265_RS08055 0.75 -1.7 2,006,140 - CA265_RS08055 0.83 -0.5 2,006,141 + CA265_RS08055 0.83 +1.5 2,006,294 - +0.7 2,006,318 + -0.2 2,006,351 - +1.6 2,006,351 - +1.6 2,006,354 - +0.0 2,006,443 + +0.8 2,006,444 - -0.2 2,006,444 - -0.5 2,006,450 - +0.1 2,006,451 + -1.0 2,006,455 + +0.1 2,006,467 - +0.3 2,006,512 - -0.1 2,006,512 - -1.2 2,006,630 - -0.8 2,006,630 - -0.6 2,006,633 - -0.3 2,006,633 - +1.6 2,006,642 + -0.5 2,006,763 + CA265_RS08060 0.41 +0.3 2,006,840 - CA265_RS08060 0.66 +0.2 2,006,897 + CA265_RS08060 0.84 +0.8 2,006,952 + +0.8 2,006,990 + +0.6 2,006,999 - -0.2 2,006,999 - +0.6 2,007,005 + +0.3 2,007,006 - +0.1 2,007,033 + +0.4 2,007,034 - +0.6 2,007,062 + +0.6 2,007,063 - +0.2 2,007,072 + -1.2 2,007,073 - +1.1 2,007,102 - CA265_RS08065 0.13 -0.3 2,007,102 - CA265_RS08065 0.13 -0.7 2,007,130 - CA265_RS08065 0.18 -0.6 2,007,230 - CA265_RS08065 0.33 -0.7 2,007,239 - CA265_RS08065 0.34 -0.3 2,007,239 - CA265_RS08065 0.34 -0.3 2,007,242 - CA265_RS08065 0.35 -1.0 2,007,253 - CA265_RS08065 0.37 +0.2
Or see this region's nucleotide sequence