Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS07520 and CA265_RS07525 overlap by 14 nucleotides CA265_RS07525 and CA265_RS07530 are separated by 8 nucleotides CA265_RS07530 and CA265_RS07535 are separated by 87 nucleotides CA265_RS07535 and CA265_RS07540 are separated by 2 nucleotides CA265_RS07540 and CA265_RS07545 are separated by 87 nucleotides
CA265_RS07520: CA265_RS07520 - cation diffusion facilitator family transporter, at 1,895,023 to 1,896,009
_RS07520
CA265_RS07525: CA265_RS07525 - NUDIX hydrolase, at 1,895,996 to 1,896,448
_RS07525
CA265_RS07530: CA265_RS07530 - adenosylmethionine--8-amino-7-oxononanoate transaminase, at 1,896,457 to 1,897,758
_RS07530
CA265_RS07535: CA265_RS07535 - ATPase, at 1,897,846 to 1,898,280
_RS07535
CA265_RS07540: CA265_RS07540 - transcriptional regulator, at 1,898,283 to 1,898,600
_RS07540
CA265_RS07545: CA265_RS07545 - extradiol dioxygenase, at 1,898,688 to 1,899,104
_RS07545
Position (kb)
1896
1897
1898 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1895.482 kb on + strand, within CA265_RS07520 at 1895.513 kb on + strand, within CA265_RS07520 at 1895.532 kb on + strand, within CA265_RS07520 at 1895.533 kb on - strand, within CA265_RS07520 at 1895.552 kb on + strand, within CA265_RS07520 at 1895.553 kb on - strand, within CA265_RS07520 at 1895.575 kb on + strand, within CA265_RS07520 at 1895.596 kb on - strand, within CA265_RS07520 at 1895.729 kb on - strand, within CA265_RS07520 at 1895.748 kb on - strand, within CA265_RS07520 at 1895.855 kb on + strand, within CA265_RS07520 at 1895.904 kb on + strand, within CA265_RS07520 at 1895.967 kb on - strand at 1895.970 kb on - strand at 1896.007 kb on + strand at 1896.007 kb on + strand at 1896.030 kb on + strand at 1896.030 kb on + strand at 1896.043 kb on + strand, within CA265_RS07525 at 1896.075 kb on - strand, within CA265_RS07525 at 1896.075 kb on - strand, within CA265_RS07525 at 1896.187 kb on - strand, within CA265_RS07525 at 1896.187 kb on - strand, within CA265_RS07525 at 1896.187 kb on - strand, within CA265_RS07525 at 1896.198 kb on - strand, within CA265_RS07525 at 1896.198 kb on - strand, within CA265_RS07525 at 1896.199 kb on + strand, within CA265_RS07525 at 1896.202 kb on + strand, within CA265_RS07525 at 1896.254 kb on + strand, within CA265_RS07525 at 1896.254 kb on + strand, within CA265_RS07525 at 1896.254 kb on + strand, within CA265_RS07525 at 1896.255 kb on - strand, within CA265_RS07525 at 1896.282 kb on + strand, within CA265_RS07525 at 1896.302 kb on - strand, within CA265_RS07525 at 1896.369 kb on + strand, within CA265_RS07525 at 1896.373 kb on + strand, within CA265_RS07525 at 1896.446 kb on + strand at 1896.504 kb on - strand at 1896.524 kb on + strand at 1896.525 kb on - strand at 1896.596 kb on - strand, within CA265_RS07530 at 1896.624 kb on + strand, within CA265_RS07530 at 1896.625 kb on - strand, within CA265_RS07530 at 1896.655 kb on - strand, within CA265_RS07530 at 1896.655 kb on - strand, within CA265_RS07530 at 1896.679 kb on - strand, within CA265_RS07530 at 1896.758 kb on + strand, within CA265_RS07530 at 1896.759 kb on - strand, within CA265_RS07530 at 1896.919 kb on + strand, within CA265_RS07530 at 1897.033 kb on - strand, within CA265_RS07530 at 1897.138 kb on + strand, within CA265_RS07530 at 1897.182 kb on - strand, within CA265_RS07530 at 1897.185 kb on + strand, within CA265_RS07530 at 1897.200 kb on - strand, within CA265_RS07530 at 1897.228 kb on + strand, within CA265_RS07530 at 1897.228 kb on + strand, within CA265_RS07530 at 1897.252 kb on - strand, within CA265_RS07530 at 1897.252 kb on - strand, within CA265_RS07530 at 1897.437 kb on + strand, within CA265_RS07530 at 1897.513 kb on - strand, within CA265_RS07530 at 1897.599 kb on - strand, within CA265_RS07530 at 1897.602 kb on + strand, within CA265_RS07530 at 1897.649 kb on + strand at 1897.650 kb on - strand at 1897.717 kb on + strand at 1897.723 kb on + strand at 1897.724 kb on - strand at 1897.756 kb on + strand at 1897.756 kb on + strand at 1897.757 kb on - strand at 1897.757 kb on - strand at 1897.757 kb on - strand at 1897.761 kb on - strand at 1897.761 kb on - strand at 1897.761 kb on - strand at 1897.777 kb on + strand at 1897.816 kb on - strand at 1897.892 kb on + strand, within CA265_RS07535 at 1898.013 kb on + strand at 1898.042 kb on + strand, within CA265_RS07535 at 1898.105 kb on + strand, within CA265_RS07535 at 1898.106 kb on - strand, within CA265_RS07535 at 1898.262 kb on + strand at 1898.262 kb on + strand at 1898.329 kb on - strand, within CA265_RS07540 at 1898.402 kb on + strand, within CA265_RS07540 at 1898.403 kb on - strand, within CA265_RS07540 at 1898.403 kb on - strand, within CA265_RS07540 at 1898.403 kb on - strand, within CA265_RS07540 at 1898.528 kb on + strand, within CA265_RS07540 at 1898.564 kb on + strand, within CA265_RS07540 at 1898.640 kb on - strand at 1898.683 kb on - strand at 1898.694 kb on + strand at 1898.695 kb on - strand at 1898.717 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 1,895,482 + CA265_RS07520 0.47 -0.0 1,895,513 + CA265_RS07520 0.50 +0.6 1,895,532 + CA265_RS07520 0.52 +0.5 1,895,533 - CA265_RS07520 0.52 +0.4 1,895,552 + CA265_RS07520 0.54 -0.4 1,895,553 - CA265_RS07520 0.54 -0.3 1,895,575 + CA265_RS07520 0.56 -0.6 1,895,596 - CA265_RS07520 0.58 -0.8 1,895,729 - CA265_RS07520 0.72 -0.5 1,895,748 - CA265_RS07520 0.73 -0.4 1,895,855 + CA265_RS07520 0.84 -0.5 1,895,904 + CA265_RS07520 0.89 +1.1 1,895,967 - +1.0 1,895,970 - -0.2 1,896,007 + +1.0 1,896,007 + -0.3 1,896,030 + -1.2 1,896,030 + -0.9 1,896,043 + CA265_RS07525 0.10 +0.5 1,896,075 - CA265_RS07525 0.17 +0.5 1,896,075 - CA265_RS07525 0.17 +0.2 1,896,187 - CA265_RS07525 0.42 +0.4 1,896,187 - CA265_RS07525 0.42 -0.3 1,896,187 - CA265_RS07525 0.42 +0.1 1,896,198 - CA265_RS07525 0.45 -0.0 1,896,198 - CA265_RS07525 0.45 +0.5 1,896,199 + CA265_RS07525 0.45 +0.7 1,896,202 + CA265_RS07525 0.45 -0.2 1,896,254 + CA265_RS07525 0.57 +0.2 1,896,254 + CA265_RS07525 0.57 +0.3 1,896,254 + CA265_RS07525 0.57 +0.4 1,896,255 - CA265_RS07525 0.57 -0.4 1,896,282 + CA265_RS07525 0.63 -0.6 1,896,302 - CA265_RS07525 0.68 -1.0 1,896,369 + CA265_RS07525 0.82 +0.2 1,896,373 + CA265_RS07525 0.83 -0.5 1,896,446 + -1.2 1,896,504 - +0.2 1,896,524 + +0.0 1,896,525 - -0.9 1,896,596 - CA265_RS07530 0.11 -0.9 1,896,624 + CA265_RS07530 0.13 -0.3 1,896,625 - CA265_RS07530 0.13 +0.6 1,896,655 - CA265_RS07530 0.15 -0.3 1,896,655 - CA265_RS07530 0.15 -0.6 1,896,679 - CA265_RS07530 0.17 -0.1 1,896,758 + CA265_RS07530 0.23 -1.7 1,896,759 - CA265_RS07530 0.23 -0.2 1,896,919 + CA265_RS07530 0.35 +0.1 1,897,033 - CA265_RS07530 0.44 +0.2 1,897,138 + CA265_RS07530 0.52 -0.1 1,897,182 - CA265_RS07530 0.56 +1.4 1,897,185 + CA265_RS07530 0.56 +0.3 1,897,200 - CA265_RS07530 0.57 +0.7 1,897,228 + CA265_RS07530 0.59 +0.1 1,897,228 + CA265_RS07530 0.59 -0.9 1,897,252 - CA265_RS07530 0.61 +0.0 1,897,252 - CA265_RS07530 0.61 +0.9 1,897,437 + CA265_RS07530 0.75 -0.5 1,897,513 - CA265_RS07530 0.81 +0.4 1,897,599 - CA265_RS07530 0.88 -0.1 1,897,602 + CA265_RS07530 0.88 +1.3 1,897,649 + +1.0 1,897,650 - -0.4 1,897,717 + +0.7 1,897,723 + -0.0 1,897,724 - -0.8 1,897,756 + -0.7 1,897,756 + +0.9 1,897,757 - -0.9 1,897,757 - +1.4 1,897,757 - -0.1 1,897,761 - -0.1 1,897,761 - -0.7 1,897,761 - +0.7 1,897,777 + +0.5 1,897,816 - +0.8 1,897,892 + CA265_RS07535 0.11 -0.7 1,898,013 + +3.4 1,898,042 + CA265_RS07535 0.45 -0.7 1,898,105 + CA265_RS07535 0.60 -0.5 1,898,106 - CA265_RS07535 0.60 +0.6 1,898,262 + +0.6 1,898,262 + -0.4 1,898,329 - CA265_RS07540 0.14 +0.3 1,898,402 + CA265_RS07540 0.37 -0.5 1,898,403 - CA265_RS07540 0.38 +0.4 1,898,403 - CA265_RS07540 0.38 -0.2 1,898,403 - CA265_RS07540 0.38 -0.8 1,898,528 + CA265_RS07540 0.77 -0.0 1,898,564 + CA265_RS07540 0.88 -1.2 1,898,640 - -0.1 1,898,683 - -0.1 1,898,694 + +0.4 1,898,695 - +0.5 1,898,717 + -0.8
Or see this region's nucleotide sequence