Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS04810 and CA265_RS04815 are separated by 318 nucleotides CA265_RS04815 and CA265_RS04820 are separated by 402 nucleotides CA265_RS04820 and CA265_RS04825 are separated by 177 nucleotides
CA265_RS04810: CA265_RS04810 - response regulator, at 1,145,557 to 1,145,919
_RS04810
CA265_RS04815: CA265_RS04815 - hypothetical protein, at 1,146,238 to 1,146,492
_RS04815
CA265_RS04820: CA265_RS04820 - GntR family transcriptional regulator, at 1,146,895 to 1,147,599
_RS04820
CA265_RS04825: CA265_RS04825 - hypothetical protein, at 1,147,777 to 1,151,112
_RS04825
Position (kb)
1146
1147
1148 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1146.003 kb on - strand at 1146.062 kb on + strand at 1146.087 kb on - strand at 1146.120 kb on - strand at 1146.149 kb on - strand at 1146.157 kb on + strand at 1146.158 kb on - strand at 1146.177 kb on + strand at 1146.177 kb on + strand at 1146.248 kb on + strand at 1146.408 kb on + strand, within CA265_RS04815 at 1146.409 kb on - strand, within CA265_RS04815 at 1146.428 kb on + strand, within CA265_RS04815 at 1146.461 kb on + strand, within CA265_RS04815 at 1146.462 kb on - strand, within CA265_RS04815 at 1146.522 kb on + strand at 1146.523 kb on - strand at 1146.566 kb on + strand at 1146.666 kb on + strand at 1146.667 kb on - strand at 1146.671 kb on - strand at 1146.671 kb on - strand at 1146.754 kb on - strand at 1146.754 kb on - strand at 1146.757 kb on + strand at 1146.758 kb on - strand at 1146.789 kb on - strand at 1146.794 kb on + strand at 1146.797 kb on + strand at 1146.798 kb on - strand at 1146.829 kb on - strand at 1146.843 kb on + strand at 1146.844 kb on - strand at 1146.859 kb on - strand at 1146.882 kb on - strand at 1147.000 kb on - strand, within CA265_RS04820 at 1147.073 kb on - strand, within CA265_RS04820 at 1147.075 kb on + strand, within CA265_RS04820 at 1147.117 kb on - strand, within CA265_RS04820 at 1147.117 kb on - strand, within CA265_RS04820 at 1147.317 kb on + strand, within CA265_RS04820 at 1147.400 kb on - strand, within CA265_RS04820 at 1147.438 kb on + strand, within CA265_RS04820 at 1147.438 kb on + strand, within CA265_RS04820 at 1147.439 kb on - strand, within CA265_RS04820 at 1147.450 kb on + strand, within CA265_RS04820 at 1147.491 kb on + strand, within CA265_RS04820 at 1147.523 kb on - strand, within CA265_RS04820 at 1147.537 kb on - strand at 1147.537 kb on - strand at 1147.579 kb on - strand at 1147.601 kb on - strand at 1147.608 kb on - strand at 1147.660 kb on - strand at 1147.665 kb on + strand at 1147.678 kb on - strand at 1147.818 kb on + strand at 1147.835 kb on + strand at 1147.884 kb on + strand at 1147.998 kb on + strand at 1147.998 kb on + strand at 1147.999 kb on - strand at 1148.050 kb on - strand at 1148.124 kb on + strand, within CA265_RS04825 at 1148.124 kb on + strand, within CA265_RS04825 at 1148.125 kb on - strand, within CA265_RS04825 at 1148.127 kb on + strand, within CA265_RS04825 at 1148.192 kb on + strand, within CA265_RS04825 at 1148.223 kb on + strand, within CA265_RS04825 at 1148.277 kb on + strand, within CA265_RS04825 at 1148.278 kb on - strand, within CA265_RS04825 at 1148.278 kb on - strand, within CA265_RS04825 at 1148.280 kb on + strand, within CA265_RS04825 at 1148.281 kb on - strand, within CA265_RS04825 at 1148.281 kb on - strand, within CA265_RS04825 at 1148.305 kb on + strand, within CA265_RS04825 at 1148.331 kb on - strand, within CA265_RS04825 at 1148.497 kb on - strand, within CA265_RS04825 at 1148.520 kb on + strand, within CA265_RS04825 at 1148.521 kb on - strand, within CA265_RS04825 at 1148.526 kb on + strand, within CA265_RS04825 at 1148.527 kb on - strand, within CA265_RS04825 at 1148.566 kb on + strand, within CA265_RS04825 at 1148.572 kb on + strand, within CA265_RS04825
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 1,146,003 - -0.4 1,146,062 + -1.4 1,146,087 - -0.0 1,146,120 - +0.0 1,146,149 - -0.1 1,146,157 + -0.3 1,146,158 - +0.1 1,146,177 + -0.8 1,146,177 + -0.6 1,146,248 + +1.6 1,146,408 + CA265_RS04815 0.67 +0.1 1,146,409 - CA265_RS04815 0.67 -0.4 1,146,428 + CA265_RS04815 0.75 -0.4 1,146,461 + CA265_RS04815 0.87 +1.0 1,146,462 - CA265_RS04815 0.88 +1.2 1,146,522 + +0.2 1,146,523 - -0.6 1,146,566 + +0.1 1,146,666 + -0.3 1,146,667 - +0.7 1,146,671 - +0.1 1,146,671 - -0.4 1,146,754 - -1.5 1,146,754 - +1.0 1,146,757 + -0.7 1,146,758 - +0.1 1,146,789 - +0.3 1,146,794 + +0.2 1,146,797 + +0.9 1,146,798 - +0.8 1,146,829 - +0.2 1,146,843 + -1.0 1,146,844 - +0.3 1,146,859 - +0.4 1,146,882 - +0.0 1,147,000 - CA265_RS04820 0.15 -2.1 1,147,073 - CA265_RS04820 0.25 -2.7 1,147,075 + CA265_RS04820 0.26 -0.5 1,147,117 - CA265_RS04820 0.31 +2.3 1,147,117 - CA265_RS04820 0.31 -3.1 1,147,317 + CA265_RS04820 0.60 -2.5 1,147,400 - CA265_RS04820 0.72 -0.5 1,147,438 + CA265_RS04820 0.77 -3.1 1,147,438 + CA265_RS04820 0.77 +1.4 1,147,439 - CA265_RS04820 0.77 -4.3 1,147,450 + CA265_RS04820 0.79 -2.2 1,147,491 + CA265_RS04820 0.85 -1.9 1,147,523 - CA265_RS04820 0.89 -0.5 1,147,537 - -2.0 1,147,537 - -2.6 1,147,579 - -1.8 1,147,601 - -1.3 1,147,608 - -1.6 1,147,660 - -0.0 1,147,665 + -2.2 1,147,678 - +1.0 1,147,818 + +0.8 1,147,835 + +0.3 1,147,884 + +0.8 1,147,998 + -0.3 1,147,998 + +2.0 1,147,999 - +1.1 1,148,050 - +1.1 1,148,124 + CA265_RS04825 0.10 +0.9 1,148,124 + CA265_RS04825 0.10 +0.8 1,148,125 - CA265_RS04825 0.10 +0.6 1,148,127 + CA265_RS04825 0.10 +0.3 1,148,192 + CA265_RS04825 0.12 +0.7 1,148,223 + CA265_RS04825 0.13 -1.9 1,148,277 + CA265_RS04825 0.15 +1.2 1,148,278 - CA265_RS04825 0.15 +0.9 1,148,278 - CA265_RS04825 0.15 +0.8 1,148,280 + CA265_RS04825 0.15 +0.2 1,148,281 - CA265_RS04825 0.15 +0.9 1,148,281 - CA265_RS04825 0.15 +1.3 1,148,305 + CA265_RS04825 0.16 +0.4 1,148,331 - CA265_RS04825 0.17 +0.0 1,148,497 - CA265_RS04825 0.22 +0.2 1,148,520 + CA265_RS04825 0.22 +1.3 1,148,521 - CA265_RS04825 0.22 +2.3 1,148,526 + CA265_RS04825 0.22 +0.7 1,148,527 - CA265_RS04825 0.22 +0.0 1,148,566 + CA265_RS04825 0.24 -0.6 1,148,572 + CA265_RS04825 0.24 +0.2
Or see this region's nucleotide sequence