Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS04145 and CA265_RS04150 are separated by 510 nucleotides CA265_RS04150 and CA265_RS04155 are separated by 271 nucleotides
CA265_RS04145: CA265_RS04145 - glucose-1-phosphate adenylyltransferase, at 997,942 to 999,210
_RS04145
CA265_RS04150: CA265_RS04150 - glycosyl hydrolase, at 999,721 to 1,001,505
_RS04150
CA265_RS04155: CA265_RS04155 - bifunctional alpha,alpha-trehalose-phosphate synthase (UDP-forming)/trehalose-phosphatase, at 1,001,777 to 1,003,966
_RS04155
Position (kb)
999
1000
1001
1002 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 998.739 kb on - strand, within CA265_RS04145 at 998.760 kb on + strand, within CA265_RS04145 at 998.769 kb on + strand, within CA265_RS04145 at 998.871 kb on + strand, within CA265_RS04145 at 998.872 kb on - strand, within CA265_RS04145 at 998.875 kb on - strand, within CA265_RS04145 at 998.891 kb on + strand, within CA265_RS04145 at 998.891 kb on + strand, within CA265_RS04145 at 998.939 kb on + strand, within CA265_RS04145 at 998.940 kb on - strand, within CA265_RS04145 at 998.957 kb on + strand, within CA265_RS04145 at 999.052 kb on - strand, within CA265_RS04145 at 999.052 kb on - strand, within CA265_RS04145 at 999.120 kb on - strand at 999.142 kb on + strand at 999.143 kb on - strand at 999.143 kb on - strand at 999.143 kb on - strand at 999.185 kb on + strand at 999.214 kb on - strand at 999.220 kb on + strand at 999.296 kb on - strand at 999.381 kb on + strand at 999.449 kb on + strand at 999.465 kb on - strand at 999.489 kb on - strand at 999.490 kb on + strand at 999.559 kb on + strand at 999.602 kb on + strand at 999.610 kb on + strand at 999.696 kb on - strand at 999.713 kb on + strand at 999.715 kb on + strand at 999.721 kb on - strand at 999.721 kb on - strand at 999.782 kb on + strand at 999.798 kb on + strand at 999.801 kb on - strand at 999.801 kb on - strand at 999.851 kb on + strand at 999.917 kb on - strand, within CA265_RS04150 at 999.917 kb on - strand, within CA265_RS04150 at 999.919 kb on + strand, within CA265_RS04150 at 999.922 kb on - strand, within CA265_RS04150 at 1000.007 kb on + strand, within CA265_RS04150 at 1000.008 kb on - strand, within CA265_RS04150 at 1000.009 kb on + strand, within CA265_RS04150 at 1000.009 kb on + strand, within CA265_RS04150 at 1000.023 kb on + strand, within CA265_RS04150 at 1000.024 kb on - strand, within CA265_RS04150 at 1000.186 kb on - strand, within CA265_RS04150 at 1000.201 kb on - strand, within CA265_RS04150 at 1000.201 kb on - strand, within CA265_RS04150 at 1000.205 kb on - strand, within CA265_RS04150 at 1000.246 kb on + strand, within CA265_RS04150 at 1000.247 kb on - strand, within CA265_RS04150 at 1000.293 kb on + strand, within CA265_RS04150 at 1000.323 kb on - strand, within CA265_RS04150 at 1000.449 kb on + strand, within CA265_RS04150 at 1000.526 kb on - strand, within CA265_RS04150 at 1000.526 kb on - strand, within CA265_RS04150 at 1000.529 kb on + strand, within CA265_RS04150 at 1000.530 kb on - strand, within CA265_RS04150 at 1000.616 kb on + strand, within CA265_RS04150 at 1000.616 kb on + strand, within CA265_RS04150 at 1000.633 kb on + strand, within CA265_RS04150 at 1000.634 kb on - strand, within CA265_RS04150 at 1000.690 kb on + strand, within CA265_RS04150 at 1000.690 kb on + strand, within CA265_RS04150 at 1000.710 kb on + strand, within CA265_RS04150 at 1000.730 kb on - strand, within CA265_RS04150 at 1000.750 kb on - strand, within CA265_RS04150 at 1000.754 kb on - strand, within CA265_RS04150 at 1000.754 kb on - strand, within CA265_RS04150 at 1000.754 kb on - strand, within CA265_RS04150 at 1000.778 kb on + strand, within CA265_RS04150 at 1000.787 kb on - strand, within CA265_RS04150 at 1000.920 kb on - strand, within CA265_RS04150 at 1000.920 kb on - strand, within CA265_RS04150 at 1000.920 kb on - strand, within CA265_RS04150 at 1001.045 kb on + strand, within CA265_RS04150 at 1001.046 kb on - strand, within CA265_RS04150 at 1001.046 kb on - strand, within CA265_RS04150 at 1001.178 kb on - strand, within CA265_RS04150 at 1001.232 kb on - strand, within CA265_RS04150 at 1001.350 kb on - strand at 1001.352 kb on + strand at 1001.353 kb on - strand at 1001.353 kb on - strand at 1001.356 kb on + strand at 1001.434 kb on + strand at 1001.478 kb on + strand at 1001.495 kb on + strand at 1001.511 kb on + strand at 1001.512 kb on - strand at 1001.561 kb on + strand at 1001.612 kb on + strand at 1001.614 kb on + strand at 1001.617 kb on - strand at 1001.620 kb on + strand at 1001.621 kb on - strand at 1001.621 kb on - strand at 1001.685 kb on + strand at 1001.685 kb on + strand at 1001.733 kb on - strand at 1001.851 kb on - strand at 1001.853 kb on + strand at 1001.890 kb on + strand at 1001.922 kb on + strand at 1001.976 kb on + strand at 1001.977 kb on - strand at 1001.977 kb on - strand at 1002.064 kb on + strand, within CA265_RS04155 at 1002.064 kb on + strand, within CA265_RS04155 at 1002.159 kb on + strand, within CA265_RS04155 at 1002.159 kb on + strand, within CA265_RS04155 at 1002.320 kb on + strand, within CA265_RS04155 at 1002.321 kb on - strand, within CA265_RS04155 at 1002.368 kb on - strand, within CA265_RS04155 at 1002.368 kb on - strand, within CA265_RS04155 at 1002.398 kb on + strand, within CA265_RS04155 at 1002.410 kb on + strand, within CA265_RS04155 at 1002.411 kb on - strand, within CA265_RS04155 at 1002.419 kb on + strand, within CA265_RS04155 at 1002.420 kb on - strand, within CA265_RS04155 at 1002.481 kb on - strand, within CA265_RS04155 at 1002.491 kb on + strand, within CA265_RS04155 at 1002.492 kb on - strand, within CA265_RS04155
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 998,739 - CA265_RS04145 0.63 -0.8 998,760 + CA265_RS04145 0.64 -0.2 998,769 + CA265_RS04145 0.65 +0.6 998,871 + CA265_RS04145 0.73 +0.1 998,872 - CA265_RS04145 0.73 +0.7 998,875 - CA265_RS04145 0.74 +0.1 998,891 + CA265_RS04145 0.75 +1.9 998,891 + CA265_RS04145 0.75 +1.2 998,939 + CA265_RS04145 0.79 -0.5 998,940 - CA265_RS04145 0.79 +0.5 998,957 + CA265_RS04145 0.80 -0.3 999,052 - CA265_RS04145 0.87 -0.8 999,052 - CA265_RS04145 0.87 +0.3 999,120 - -0.3 999,142 + -0.4 999,143 - -0.5 999,143 - -2.2 999,143 - -0.7 999,185 + +0.5 999,214 - -0.1 999,220 + -0.3 999,296 - +1.2 999,381 + +0.4 999,449 + +0.2 999,465 - -0.4 999,489 - -0.3 999,490 + -0.1 999,559 + -0.6 999,602 + +1.4 999,610 + +0.2 999,696 - +0.2 999,713 + +1.1 999,715 + -0.4 999,721 - +0.4 999,721 - -0.3 999,782 + +0.9 999,798 + +0.1 999,801 - +0.4 999,801 - +0.2 999,851 + -0.3 999,917 - CA265_RS04150 0.11 +0.5 999,917 - CA265_RS04150 0.11 -0.5 999,919 + CA265_RS04150 0.11 +1.0 999,922 - CA265_RS04150 0.11 -0.1 1,000,007 + CA265_RS04150 0.16 +0.5 1,000,008 - CA265_RS04150 0.16 +0.3 1,000,009 + CA265_RS04150 0.16 +0.6 1,000,009 + CA265_RS04150 0.16 +2.3 1,000,023 + CA265_RS04150 0.17 -1.4 1,000,024 - CA265_RS04150 0.17 +0.1 1,000,186 - CA265_RS04150 0.26 +0.1 1,000,201 - CA265_RS04150 0.27 +1.0 1,000,201 - CA265_RS04150 0.27 +0.9 1,000,205 - CA265_RS04150 0.27 +1.3 1,000,246 + CA265_RS04150 0.29 -0.4 1,000,247 - CA265_RS04150 0.29 +0.0 1,000,293 + CA265_RS04150 0.32 -0.4 1,000,323 - CA265_RS04150 0.34 -1.3 1,000,449 + CA265_RS04150 0.41 +1.1 1,000,526 - CA265_RS04150 0.45 +0.1 1,000,526 - CA265_RS04150 0.45 -0.0 1,000,529 + CA265_RS04150 0.45 +1.0 1,000,530 - CA265_RS04150 0.45 -0.3 1,000,616 + CA265_RS04150 0.50 -0.0 1,000,616 + CA265_RS04150 0.50 +0.3 1,000,633 + CA265_RS04150 0.51 +0.4 1,000,634 - CA265_RS04150 0.51 -0.0 1,000,690 + CA265_RS04150 0.54 +1.9 1,000,690 + CA265_RS04150 0.54 -0.1 1,000,710 + CA265_RS04150 0.55 +0.4 1,000,730 - CA265_RS04150 0.57 -0.1 1,000,750 - CA265_RS04150 0.58 +0.1 1,000,754 - CA265_RS04150 0.58 +0.4 1,000,754 - CA265_RS04150 0.58 +1.3 1,000,754 - CA265_RS04150 0.58 +1.3 1,000,778 + CA265_RS04150 0.59 +0.8 1,000,787 - CA265_RS04150 0.60 +0.3 1,000,920 - CA265_RS04150 0.67 +0.2 1,000,920 - CA265_RS04150 0.67 +1.1 1,000,920 - CA265_RS04150 0.67 -0.9 1,001,045 + CA265_RS04150 0.74 +0.3 1,001,046 - CA265_RS04150 0.74 +1.2 1,001,046 - CA265_RS04150 0.74 -1.2 1,001,178 - CA265_RS04150 0.82 +0.1 1,001,232 - CA265_RS04150 0.85 -2.8 1,001,350 - -0.2 1,001,352 + -0.6 1,001,353 - -3.0 1,001,353 - +0.5 1,001,356 + +1.2 1,001,434 + -0.6 1,001,478 + +0.5 1,001,495 + -1.3 1,001,511 + -0.3 1,001,512 - +0.0 1,001,561 + +0.3 1,001,612 + +0.7 1,001,614 + +0.3 1,001,617 - +0.1 1,001,620 + +1.2 1,001,621 - +0.8 1,001,621 - +0.4 1,001,685 + -0.5 1,001,685 + +0.1 1,001,733 - -0.6 1,001,851 - +0.3 1,001,853 + +0.4 1,001,890 + -0.1 1,001,922 + +0.3 1,001,976 + -0.0 1,001,977 - +0.9 1,001,977 - -0.2 1,002,064 + CA265_RS04155 0.13 -0.7 1,002,064 + CA265_RS04155 0.13 -0.1 1,002,159 + CA265_RS04155 0.17 -1.2 1,002,159 + CA265_RS04155 0.17 -0.4 1,002,320 + CA265_RS04155 0.25 -1.8 1,002,321 - CA265_RS04155 0.25 +0.0 1,002,368 - CA265_RS04155 0.27 +0.8 1,002,368 - CA265_RS04155 0.27 +0.8 1,002,398 + CA265_RS04155 0.28 -0.4 1,002,410 + CA265_RS04155 0.29 -0.2 1,002,411 - CA265_RS04155 0.29 -0.8 1,002,419 + CA265_RS04155 0.29 +0.8 1,002,420 - CA265_RS04155 0.29 -0.0 1,002,481 - CA265_RS04155 0.32 +0.6 1,002,491 + CA265_RS04155 0.33 -0.6 1,002,492 - CA265_RS04155 0.33 +0.2
Or see this region's nucleotide sequence