Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS01515 and CA265_RS01520 are separated by 364 nucleotides CA265_RS01520 and CA265_RS01525 are separated by 152 nucleotides
CA265_RS01515: CA265_RS01515 - 1,4-beta-xylanase, at 387,402 to 388,448
_RS01515
CA265_RS01520: CA265_RS01520 - beta-galactosidase, at 388,813 to 390,663
_RS01520
CA265_RS01525: CA265_RS01525 - hypothetical protein, at 390,816 to 391,262
_RS01525
Position (kb) 388
389
390
391 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 387.895 kb on - strand, within CA265_RS01515 at 387.895 kb on - strand, within CA265_RS01515 at 387.907 kb on + strand, within CA265_RS01515 at 387.907 kb on + strand, within CA265_RS01515 at 387.982 kb on + strand, within CA265_RS01515 at 388.116 kb on - strand, within CA265_RS01515 at 388.126 kb on - strand, within CA265_RS01515 at 388.131 kb on - strand, within CA265_RS01515 at 388.134 kb on + strand, within CA265_RS01515 at 388.135 kb on - strand, within CA265_RS01515 at 388.209 kb on + strand, within CA265_RS01515 at 388.314 kb on - strand, within CA265_RS01515 at 388.320 kb on + strand, within CA265_RS01515 at 388.320 kb on + strand, within CA265_RS01515 at 388.321 kb on - strand, within CA265_RS01515 at 388.540 kb on - strand at 388.675 kb on + strand at 388.675 kb on + strand at 388.676 kb on - strand at 388.697 kb on + strand at 388.793 kb on + strand at 388.837 kb on - strand at 388.935 kb on + strand at 388.936 kb on - strand at 388.989 kb on + strand at 389.087 kb on + strand, within CA265_RS01520 at 389.124 kb on + strand, within CA265_RS01520 at 389.157 kb on + strand, within CA265_RS01520 at 389.241 kb on + strand, within CA265_RS01520 at 389.241 kb on + strand, within CA265_RS01520 at 389.254 kb on - strand, within CA265_RS01520 at 389.254 kb on - strand, within CA265_RS01520 at 389.290 kb on - strand, within CA265_RS01520 at 389.334 kb on - strand, within CA265_RS01520 at 389.673 kb on - strand, within CA265_RS01520 at 389.706 kb on + strand, within CA265_RS01520 at 389.714 kb on + strand, within CA265_RS01520 at 389.775 kb on + strand, within CA265_RS01520 at 389.785 kb on - strand, within CA265_RS01520 at 389.815 kb on + strand, within CA265_RS01520 at 389.917 kb on - strand, within CA265_RS01520 at 389.917 kb on - strand, within CA265_RS01520 at 389.919 kb on + strand, within CA265_RS01520 at 389.937 kb on + strand, within CA265_RS01520 at 389.942 kb on + strand, within CA265_RS01520 at 390.097 kb on + strand, within CA265_RS01520 at 390.242 kb on + strand, within CA265_RS01520 at 390.301 kb on + strand, within CA265_RS01520 at 390.302 kb on - strand, within CA265_RS01520 at 390.331 kb on + strand, within CA265_RS01520 at 390.332 kb on - strand, within CA265_RS01520 at 390.466 kb on + strand, within CA265_RS01520 at 390.467 kb on - strand, within CA265_RS01520 at 390.574 kb on + strand at 390.574 kb on + strand at 390.574 kb on + strand at 390.575 kb on - strand at 390.621 kb on - strand at 390.639 kb on + strand at 390.642 kb on + strand at 390.672 kb on - strand at 390.730 kb on + strand at 390.730 kb on + strand at 390.732 kb on + strand at 390.740 kb on - strand at 390.740 kb on - strand at 390.803 kb on - strand at 391.010 kb on - strand, within CA265_RS01525 at 391.023 kb on - strand, within CA265_RS01525 at 391.078 kb on - strand, within CA265_RS01525 at 391.085 kb on + strand, within CA265_RS01525 at 391.186 kb on + strand, within CA265_RS01525 at 391.187 kb on - strand, within CA265_RS01525 at 391.212 kb on + strand, within CA265_RS01525 at 391.236 kb on - strand at 391.236 kb on - strand at 391.238 kb on + strand at 391.243 kb on - strand at 391.267 kb on + strand at 391.283 kb on - strand at 391.329 kb on - strand at 391.329 kb on - strand at 391.367 kb on - strand at 391.408 kb on - strand at 391.408 kb on - strand at 391.446 kb on + strand at 391.526 kb on - strand at 391.531 kb on + strand at 391.531 kb on + strand at 391.531 kb on + strand at 391.531 kb on + strand at 391.532 kb on - strand at 391.532 kb on - strand at 391.547 kb on - strand at 391.557 kb on - strand at 391.564 kb on + strand at 391.598 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 387,895 - CA265_RS01515 0.47 -0.2 387,895 - CA265_RS01515 0.47 +0.4 387,907 + CA265_RS01515 0.48 +0.1 387,907 + CA265_RS01515 0.48 -0.1 387,982 + CA265_RS01515 0.55 -1.1 388,116 - CA265_RS01515 0.68 +1.2 388,126 - CA265_RS01515 0.69 +2.1 388,131 - CA265_RS01515 0.70 -0.3 388,134 + CA265_RS01515 0.70 -0.2 388,135 - CA265_RS01515 0.70 +0.2 388,209 + CA265_RS01515 0.77 +0.1 388,314 - CA265_RS01515 0.87 -0.5 388,320 + CA265_RS01515 0.88 +0.9 388,320 + CA265_RS01515 0.88 +0.2 388,321 - CA265_RS01515 0.88 +0.1 388,540 - -0.3 388,675 + -0.7 388,675 + +1.1 388,676 - +1.8 388,697 + +0.7 388,793 + -1.0 388,837 - +0.1 388,935 + +0.6 388,936 - +0.1 388,989 + +0.7 389,087 + CA265_RS01520 0.15 +0.1 389,124 + CA265_RS01520 0.17 -1.3 389,157 + CA265_RS01520 0.19 +0.2 389,241 + CA265_RS01520 0.23 -1.6 389,241 + CA265_RS01520 0.23 +0.2 389,254 - CA265_RS01520 0.24 +1.1 389,254 - CA265_RS01520 0.24 +1.2 389,290 - CA265_RS01520 0.26 -0.9 389,334 - CA265_RS01520 0.28 -0.1 389,673 - CA265_RS01520 0.46 +0.5 389,706 + CA265_RS01520 0.48 +0.2 389,714 + CA265_RS01520 0.49 -0.3 389,775 + CA265_RS01520 0.52 -0.3 389,785 - CA265_RS01520 0.53 +0.2 389,815 + CA265_RS01520 0.54 -0.3 389,917 - CA265_RS01520 0.60 +1.8 389,917 - CA265_RS01520 0.60 -0.8 389,919 + CA265_RS01520 0.60 -1.1 389,937 + CA265_RS01520 0.61 -0.1 389,942 + CA265_RS01520 0.61 +1.2 390,097 + CA265_RS01520 0.69 +0.1 390,242 + CA265_RS01520 0.77 -1.3 390,301 + CA265_RS01520 0.80 -0.8 390,302 - CA265_RS01520 0.80 -0.5 390,331 + CA265_RS01520 0.82 +0.6 390,332 - CA265_RS01520 0.82 -2.8 390,466 + CA265_RS01520 0.89 -0.4 390,467 - CA265_RS01520 0.89 +0.1 390,574 + +0.4 390,574 + +0.2 390,574 + -1.7 390,575 - +0.2 390,621 - +1.6 390,639 + +0.5 390,642 + +0.4 390,672 - +0.1 390,730 + +0.1 390,730 + -1.1 390,732 + -0.3 390,740 - +1.0 390,740 - +2.1 390,803 - +0.6 391,010 - CA265_RS01525 0.43 +0.1 391,023 - CA265_RS01525 0.46 +0.1 391,078 - CA265_RS01525 0.59 +1.6 391,085 + CA265_RS01525 0.60 +0.4 391,186 + CA265_RS01525 0.83 -1.0 391,187 - CA265_RS01525 0.83 -0.0 391,212 + CA265_RS01525 0.89 -0.8 391,236 - +0.3 391,236 - +0.4 391,238 + -0.1 391,243 - -0.1 391,267 + -0.5 391,283 - +0.2 391,329 - -0.3 391,329 - +0.5 391,367 - +0.5 391,408 - +0.1 391,408 - +0.8 391,446 + -1.9 391,526 - -0.4 391,531 + +0.2 391,531 + -0.6 391,531 + -0.1 391,531 + -0.8 391,532 - +0.4 391,532 - -0.1 391,547 - +0.8 391,557 - +0.9 391,564 + +0.2 391,598 - -0.2
Or see this region's nucleotide sequence