Strain Fitness in Acidovorax sp. GW101-3H11 around Ac3H11_3019

Experiment: nitrite 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAc3H11_3017 and Ac3H11_3018 are separated by 29 nucleotidesAc3H11_3018 and Ac3H11_3019 are separated by 27 nucleotidesAc3H11_3019 and Ac3H11_3020 are separated by 29 nucleotidesAc3H11_3020 and Ac3H11_3021 are separated by 80 nucleotides Ac3H11_3017 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 39,837 to 40,766 _3017 Ac3H11_3018 - Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4), at 40,796 to 41,710 _3018 Ac3H11_3019 - Uncharacterized protein SCO3165, at 41,738 to 42,247 _3019 Ac3H11_3020 - protein of unknown function DUF1289, at 42,277 to 42,501 _3020 Ac3H11_3021 - 2-hydroxychromene-2-carboxylate isomerase, at 42,582 to 43,235 _3021 Position (kb) 41 42 43Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 40.762 kb on - strandat 42.263 kb on + strandat 43.153 kb on - strand, within Ac3H11_3021at 43.219 kb on + strandat 43.219 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrite 10 mM
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40,762 - +2.2
42,263 + -1.1
43,153 - Ac3H11_3021 0.87 -3.1
43,219 + -1.1
43,219 + +1.5

Or see this region's nucleotide sequence