Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS03130 and AZOBR_RS03135 are separated by 260 nucleotides AZOBR_RS03135 and AZOBR_RS03140 are separated by 12 nucleotides AZOBR_RS03140 and AZOBR_RS03145 are separated by 184 nucleotides
AZOBR_RS03130: AZOBR_RS03130 - hypothetical protein, at 667,704 to 668,327
_RS03130
AZOBR_RS03135: AZOBR_RS03135 - orotidine 5'-phosphate decarboxylase, at 668,588 to 669,301
_RS03135
AZOBR_RS03140: AZOBR_RS03140 - N-(5'-phosphoribosyl)anthranilate isomerase, at 669,314 to 669,958
_RS03140
AZOBR_RS03145: AZOBR_RS03145 - diguanylate cyclase, at 670,143 to 671,018
_RS03145
Position (kb) 668
669
670 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 667.731 kb on + strand at 667.731 kb on + strand at 667.732 kb on - strand at 668.128 kb on - strand, within AZOBR_RS03130 at 668.214 kb on - strand, within AZOBR_RS03130 at 668.539 kb on + strand at 668.868 kb on + strand, within AZOBR_RS03135 at 668.869 kb on - strand at 668.869 kb on - strand, within AZOBR_RS03135 at 668.869 kb on - strand at 669.299 kb on + strand at 669.299 kb on + strand at 669.299 kb on + strand at 669.299 kb on + strand at 669.300 kb on - strand at 669.313 kb on + strand at 669.390 kb on - strand at 670.036 kb on + strand at 670.037 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 667,731 + +0.6 667,731 + -0.2 667,732 - -0.7 668,128 - AZOBR_RS03130 0.68 -0.1 668,214 - AZOBR_RS03130 0.82 +0.5 668,539 + +0.5 668,868 + AZOBR_RS03135 0.39 -2.0 668,869 - +0.0 668,869 - AZOBR_RS03135 0.39 -0.5 668,869 - -0.5 669,299 + +1.0 669,299 + -0.7 669,299 + +0.2 669,299 + -0.9 669,300 - -1.3 669,313 + -3.2 669,390 - +1.0 670,036 + -0.2 670,037 - -0.3
Or see this region's nucleotide sequence